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. 1992 Aug;174(15):5136–5140. doi: 10.1128/jb.174.15.5136-5140.1992

Production of an extracellular milk-clotting activity during development in Myxococcus xanthus.

F Petit 1, J F Guespin-Michel 1
PMCID: PMC206333  PMID: 1629171

Abstract

We describe here an extracellular proteolytic activity secreted during both growth and submerged development by Myxococcus xanthus DK1622. This activity yields the clotting of kappa-casein at pH 6 and is inhibited by specific inhibitors of aspartic proteases. Secretion of this milk-clotting proteolytic activity (of Mcp) is time regulated during the developmental cycle, with a large increase near 9 h poststarvation, but its production does not require cell-cell contact. The lack of secretion of this activity by several developmental mutants in submerged development conditions shows that Mcp production is developmentally regulated.

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Selected References

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