Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Aug;174(16):5471–5474. doi: 10.1128/jb.174.16.5471-5474.1992

Genetic evidence for an activator required for induction of pectin lyase in Erwinia carotovora subsp. carotovora by DNA-damaging agents.

J L McEvoy 1, H Murata 1, A K Chatterjee 1
PMCID: PMC206390  PMID: 1644776

Abstract

In Erwinia carotovora subsp. carotovora 71, the induction of pectin lyase (Pnl), the bacteriocin carotovoricin (Ctv), and cellular lysis (Lss) requires a RecA function. We obtained mutants wherein a pleiotropic defect, i.e., the lack of induction with mitomycin C, is not restored by the recA+ DNA. From a genomic library of strain 71, a cosmid (pAKC280) that restored induction of Pnl, Ctv, and Lss by mitomycin C was isolated. The activator function, designated Rdg for regulator of damage-inducible genes, was localized by subcloning and insertional mutagenesis to a 2.6-kb region within a 6.7-kb EcoRI fragment. An rdg-lacZ operon fusion was inducible by mitomycin C in RecA+ but not RecA- derivatives of E. carotovora subsp. carotovora 71 and Escherichia coli. A RecA+ E. coli strain carrying only a PnlA+ plasmid was not inducible for Pnl production; however, when both a PnlA+ plasmid and a Rdg+ plasmid were present, the transcription of pnlA and the production of the enzyme were activated by mitomycin C. The size of the pnlA transcript produced in E. coli was identical to that of the transcript produced by E. carotovora subsp. carotovora 71, suggesting that the same promoter and termination sequences were being utilized in these bacteria.

Full text

PDF
5471

Images in this article

5473Image
on p.5473

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aiba H., Adhya S., de Crombrugghe B. Evidence for two functional gal promoters in intact Escherichia coli cells. J Biol Chem. 1981 Nov 25;256(22):11905–11910. [PubMed] [Google Scholar]
  2. Barbé J., Vericat J. A., Llagostera M., Guerrero R. Expression of the SOS genes of Escherichia coli in Salmonella typhimurium. Microbiologia. 1985 Sep;1(1-2):77–87. [PubMed] [Google Scholar]
  3. Calero S., Garriga X., Barbé J. One-step cloning system for isolation of bacterial lexA-like genes. J Bacteriol. 1991 Nov;173(22):7345–7350. doi: 10.1128/jb.173.22.7345-7350.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Calsou P., Villaverde A., Defais M. Activated RecA protein may induce expression of a gene that is not controlled by the LexA repressor and whose function is required for mutagenesis and repair of UV-irradiated bacteriophage lambda. J Bacteriol. 1987 Oct;169(10):4816–4821. doi: 10.1128/jb.169.10.4816-4821.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Castilho B. A., Olfson P., Casadaban M. J. Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons. J Bacteriol. 1984 May;158(2):488–495. doi: 10.1128/jb.158.2.488-495.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chatterjee A., McEvoy J. L., Chambost J. P., Blasco F., Chatterjee A. K. Nucleotide sequence and molecular characterization of pnlA, the structural gene for damage-inducible pectin lyase of Erwinia carotovora subsp. carotovora 71. J Bacteriol. 1991 Mar;173(5):1765–1769. doi: 10.1128/jb.173.5.1765-1769.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Keen N. T., Tamaki S., Kobayashi D., Trollinger D. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene. 1988 Oct 15;70(1):191–197. doi: 10.1016/0378-1119(88)90117-5. [DOI] [PubMed] [Google Scholar]
  8. Little J. W., Edmiston S. H., Pacelli L. Z., Mount D. W. Cleavage of the Escherichia coli lexA protein by the recA protease. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3225–3229. doi: 10.1073/pnas.77.6.3225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Little J. W. Mechanism of specific LexA cleavage: autodigestion and the role of RecA coprotease. Biochimie. 1991 Apr;73(4):411–421. doi: 10.1016/0300-9084(91)90108-d. [DOI] [PubMed] [Google Scholar]
  10. Little J. W. The SOS regulatory system: control of its state by the level of RecA protease. J Mol Biol. 1983 Jul 15;167(4):791–808. doi: 10.1016/s0022-2836(83)80111-9. [DOI] [PubMed] [Google Scholar]
  11. McEvoy J. L., Murata H., Chatterjee A. K. Molecular cloning and characterization of an Erwinia carotovora subsp. carotovora pectin lyase gene that responds to DNA-damaging agents. J Bacteriol. 1990 Jun;172(6):3284–3289. doi: 10.1128/jb.172.6.3284-3289.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murata H., Fons M., Chatterjee A., Collmer A., Chatterjee A. K. Characterization of transposon insertion out- mutants of Erwinia carotovora subsp. carotovora defective in enzyme export and of a DNA segment that complements out mutations in E. carotovora subsp. carotovora, E. carotovora subsp. atroseptica, and Erwinia chrysanthemi. J Bacteriol. 1990 Jun;172(6):2970–2978. doi: 10.1128/jb.172.6.2970-2978.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nohmi T., Battista J. R., Dodson L. A., Walker G. C. RecA-mediated cleavage activates UmuD for mutagenesis: mechanistic relationship between transcriptional derepression and posttranslational activation. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1816–1820. doi: 10.1073/pnas.85.6.1816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Raymond-Denise A., Guillen N. Identification of dinR, a DNA damage-inducible regulator gene of Bacillus subtilis. J Bacteriol. 1991 Nov;173(22):7084–7091. doi: 10.1128/jb.173.22.7084-7091.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sedgwick S. G., Goodwin P. A. Interspecies regulation of the SOS response by the E. coli lexA+ gene. Mutat Res. 1985 May;145(3):103–106. doi: 10.1016/0167-8817(85)90015-x. [DOI] [PubMed] [Google Scholar]
  16. Selvaraj G., Fong Y. C., Iyer V. N. A portable DNA sequence carrying the cohesive site (cos) of bacteriophage lambda and the mob (mobilization) region of the broad-host-range plasmid RK2: a module for the construction of new cosmids. Gene. 1984 Dec;32(1-2):235–241. doi: 10.1016/0378-1119(84)90051-9. [DOI] [PubMed] [Google Scholar]
  17. Shinagawa H., Iwasaki H., Kato T., Nakata A. RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1806–1810. doi: 10.1073/pnas.85.6.1806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Smith C. M., Arany Z., Orrego C., Eisenstadt E. DNA damage-inducible loci in Salmonella typhimurium. J Bacteriol. 1991 Jun;173(11):3587–3590. doi: 10.1128/jb.173.11.3587-3590.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wojciechowski M. F., Peterson K. R., Love P. E. Regulation of the SOS response in Bacillus subtilis: evidence for a LexA repressor homolog. J Bacteriol. 1991 Oct;173(20):6489–6498. doi: 10.1128/jb.173.20.6489-6498.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zink R. T., Engwall J. K., McEvoy J. L., Chatterjee A. K. recA is required in the induction of pectin lyase and carotovoricin in Erwinia carotovora subsp. carotovora. J Bacteriol. 1985 Oct;164(1):390–396. doi: 10.1128/jb.164.1.390-396.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Zink R. T., Kemble R. J., Chatterjee A. K. Transposon Tn5 mutagenesis in Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica. J Bacteriol. 1984 Mar;157(3):809–814. doi: 10.1128/jb.157.3.809-814.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES