Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Sep;174(17):5633–5638. doi: 10.1128/jb.174.17.5633-5638.1992

New thermosensitive plasmid for gram-positive bacteria.

E Maguin 1, P Duwat 1, T Hege 1, D Ehrlich 1, A Gruss 1
PMCID: PMC206509  PMID: 1324906

Abstract

We isolated a replication-thermosensitive mutant of the broad-host-range replicon pWV01. The mutant pVE6002 is fully thermosensitive above 35 degrees C in both gram-negative and gram-positive bacteria. Four clustered mutations were identified in the gene encoding the replication protein of pVE6002. The thermosensitive derivative of the related plasmid pE194 carries a mutation in the analogous region but not in the same position. Derivatives of the thermosensitive plasmid convenient for cloning purposes have been constructed. The low shut-off temperature of pVE6002 makes it a useful suicide vector for bacteria which are limited in their own temperature growth range. Using pVE6002 as the delivery vector for a transposon Tn10 derivative in Bacillus subtilis, we observed transposition frequencies of about 1%.

Full text

PDF
5633

Images in this article

5635Image
on p.5635
5636Image
on p.5636

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bates E. E., Gilbert H. J. Characterization of a cryptic plasmid from Lactobacillus plantarum. Gene. 1989 Dec 21;85(1):253–258. doi: 10.1016/0378-1119(89)90491-5. [DOI] [PubMed] [Google Scholar]
  2. Bergemann A. D., Whitley J. C., Finch L. R. Homology of mycoplasma plasmid pADB201 and staphylococcal plasmid pE194. J Bacteriol. 1989 Jan;171(1):593–595. doi: 10.1128/jb.171.1.593-595.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bravo A., Alonso J. C. The generation of concatemeric plasmid DNA in Bacillus subtilis as a consequence of bacteriophage SPP1 infection. Nucleic Acids Res. 1990 Aug 25;18(16):4651–4657. doi: 10.1093/nar/18.16.4651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Byeon W. H., Weisblum B. Replication genes of plasmid pE194-cop and repF: transcripts and encoded proteins. J Bacteriol. 1990 Oct;172(10):5892–5900. doi: 10.1128/jb.172.10.5892-5900.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chopin A., Chopin M. C., Moillo-Batt A., Langella P. Two plasmid-determined restriction and modification systems in Streptococcus lactis. Plasmid. 1984 May;11(3):260–263. doi: 10.1016/0147-619x(84)90033-7. [DOI] [PubMed] [Google Scholar]
  6. Chopin M. C., Chopin A., Rouault A., Galleron N. Insertion and amplification of foreign genes in the Lactococcus lactis subsp. lactis chromosome. Appl Environ Microbiol. 1989 Jul;55(7):1769–1774. doi: 10.1128/aem.55.7.1769-1774.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Corpet F. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. 1988 Nov 25;16(22):10881–10890. doi: 10.1093/nar/16.22.10881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gasson M. J. In vivo genetic systems in lactic acid bacteria. FEMS Microbiol Rev. 1990 Sep;7(1-2):43–60. doi: 10.1111/j.1574-6968.1990.tb04878.x. [DOI] [PubMed] [Google Scholar]
  9. Gasson M. J. Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J Bacteriol. 1983 Apr;154(1):1–9. doi: 10.1128/jb.154.1.1-9.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gruss A., Ehrlich S. D. The family of highly interrelated single-stranded deoxyribonucleic acid plasmids. Microbiol Rev. 1989 Jun;53(2):231–241. doi: 10.1128/mr.53.2.231-241.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hamilton C. M., Aldea M., Washburn B. K., Babitzke P., Kushner S. R. New method for generating deletions and gene replacements in Escherichia coli. J Bacteriol. 1989 Sep;171(9):4617–4622. doi: 10.1128/jb.171.9.4617-4622.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Holo H., Nes I. F. High-Frequency Transformation, by Electroporation, of Lactococcus lactis subsp. cremoris Grown with Glycine in Osmotically Stabilized Media. Appl Environ Microbiol. 1989 Dec;55(12):3119–3123. doi: 10.1128/aem.55.12.3119-3123.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Horinouchi S., Weisblum B. Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibodies. J Bacteriol. 1982 May;150(2):804–814. doi: 10.1128/jb.150.2.804-814.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kleanthous H., Clayton C. L., Tabaqchali S. Characterization of a plasmid from Helicobacter pylori encoding a replication protein common to plasmids in gram-positive bacteria. Mol Microbiol. 1991 Oct;5(10):2377–2389. doi: 10.1111/j.1365-2958.1991.tb02084.x. [DOI] [PubMed] [Google Scholar]
  15. Kok J., van der Vossen J. M., Venema G. Construction of plasmid cloning vectors for lactic streptococci which also replicate in Bacillus subtilis and Escherichia coli. Appl Environ Microbiol. 1984 Oct;48(4):726–731. doi: 10.1128/aem.48.4.726-731.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Krah E. R., 3rd, Macrina F. L. Identification of a region that influences host range of the streptococcal conjugative plasmid pIP501. Plasmid. 1991 Jan;25(1):64–69. doi: 10.1016/0147-619x(91)90007-j. [DOI] [PubMed] [Google Scholar]
  17. Lacks S. A., Lopez P., Greenberg B., Espinosa M. Identification and analysis of genes for tetracycline resistance and replication functions in the broad-host-range plasmid pLS1. J Mol Biol. 1986 Dec 20;192(4):753–765. doi: 10.1016/0022-2836(86)90026-4. [DOI] [PubMed] [Google Scholar]
  18. Langella P., Chopin A. Conjugal transfer of plasmid pIP501 from Lactococcus lactis to Lactobacillus delbrückii subsp. bulgaricus and Lactobacillus helveticus. FEMS Microbiol Lett. 1989 Jul 15;51(1):149–152. doi: 10.1016/0378-1097(89)90498-9. [DOI] [PubMed] [Google Scholar]
  19. Le Bourgeois P., Mata M., Ritzenthaler P. Genome comparison of Lactococcus strains by pulsed-field gel electrophoresis. FEMS Microbiol Lett. 1989 May;50(1-2):65–69. doi: 10.1016/0378-1097(89)90460-6. [DOI] [PubMed] [Google Scholar]
  20. Leenhouts K. J., Kok J., Venema G. Campbell-like integration of heterologous plasmid DNA into the chromosome of Lactococcus lactis subsp. lactis. Appl Environ Microbiol. 1989 Feb;55(2):394–400. doi: 10.1128/aem.55.2.394-400.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Leenhouts K. J., Tolner B., Bron S., Kok J., Venema G., Seegers J. F. Nucleotide sequence and characterization of the broad-host-range lactococcal plasmid pWVO1. Plasmid. 1991 Jul;26(1):55–66. doi: 10.1016/0147-619x(91)90036-v. [DOI] [PubMed] [Google Scholar]
  22. Luchansky J. B., Muriana P. M., Klaenhammer T. R. Application of electroporation for transfer of plasmid DNA to Lactobacillus, Lactococcus, Leuconostoc, Listeria, Pediococcus, Bacillus, Staphylococcus, Enterococcus and Propionibacterium. Mol Microbiol. 1988 Sep;2(5):637–646. doi: 10.1111/j.1365-2958.1988.tb00072.x. [DOI] [PubMed] [Google Scholar]
  23. McKay L. L., Baldwin K. A. Applications for biotechnology: present and future improvements in lactic acid bacteria. FEMS Microbiol Rev. 1990 Sep;7(1-2):3–14. doi: 10.1111/j.1574-6968.1990.tb04876.x. [DOI] [PubMed] [Google Scholar]
  24. Mercenier A., Chassy B. M. Strategies for the development of bacterial transformation systems. Biochimie. 1988 Apr;70(4):503–517. doi: 10.1016/0300-9084(88)90086-7. [DOI] [PubMed] [Google Scholar]
  25. Minton N. P., Oultram J. D., Brehm J. K., Atkinson T. The replication proteins of plasmids pE194 and pLS1 have N-terminal homology. Nucleic Acids Res. 1988 Apr 11;16(7):3101–3101. doi: 10.1093/nar/16.7.3101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Niaudet B., Ehrlich S. D. In vitro genetic labeling of Bacillus subtilis cryptic plasmid pHV400. Plasmid. 1979 Jan;2(1):48–58. doi: 10.1016/0147-619x(79)90005-2. [DOI] [PubMed] [Google Scholar]
  27. Noirot P., Petit M. A., Ehrlich S. D. Plasmid replication stimulates DNA recombination in Bacillus subtilis. J Mol Biol. 1987 Jul 5;196(1):39–48. doi: 10.1016/0022-2836(87)90509-2. [DOI] [PubMed] [Google Scholar]
  28. Novick R. P., Edelman I., Lofdahl S. Small Staphylococcus aureus plasmids are transduced as linear multimers that are formed and resolved by replicative processes. J Mol Biol. 1986 Nov 20;192(2):209–220. doi: 10.1016/0022-2836(86)90360-8. [DOI] [PubMed] [Google Scholar]
  29. Novick R. P. Penicillinase plasmids of Staphylococcus aureus. Fed Proc. 1967 Jan-Feb;26(1):29–38. [PubMed] [Google Scholar]
  30. Otto R., de Vos W. M., Gavrieli J. Plasmid DNA in Streptococcus cremoris Wg2: Influence of pH on Selection in Chemostats of a Variant Lacking a Protease Plasmid. Appl Environ Microbiol. 1982 Jun;43(6):1272–1277. doi: 10.1128/aem.43.6.1272-1277.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Petit M. A., Bruand C., Jannière L., Ehrlich S. D. Tn10-derived transposons active in Bacillus subtilis. J Bacteriol. 1990 Dec;172(12):6736–6740. doi: 10.1128/jb.172.12.6736-6740.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Petit M. A., Mesas J. M., Noirot P., Morel-Deville F., Ehrlich S. D. Induction of DNA amplification in the Bacillus subtilis chromosome. EMBO J. 1992 Apr;11(4):1317–1326. doi: 10.1002/j.1460-2075.1992.tb05176.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Projan S. J., Carleton S., Novick R. P. Determination of plasmid copy number by fluorescence densitometry. Plasmid. 1983 Mar;9(2):182–190. doi: 10.1016/0147-619x(83)90019-7. [DOI] [PubMed] [Google Scholar]
  34. Puyet A., del Solar G. H., Espinosa M. Identification of the origin and direction of replication of the broad-host-range plasmid pLS1. Nucleic Acids Res. 1988 Jan 11;16(1):115–133. doi: 10.1093/nar/16.1.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Raya R. R., Klaenhammer T. R. High-Frequency Plasmid Transduction by Lactobacillus gasseri Bacteriophage phiadh. Appl Environ Microbiol. 1992 Jan;58(1):187–193. doi: 10.1128/aem.58.1.187-193.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Romero D. A., Slos P., Robert C., Castellino I., Mercenier A. Conjugative mobilization as an alternative vector delivery system for lactic streptococci. Appl Environ Microbiol. 1987 Oct;53(10):2405–2413. doi: 10.1128/aem.53.10.2405-2413.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Scheer-Abramowitz J., Gryczan T. J., Dubnau D. Origin and mode of replication of plasmids pE194 and pUB110. Plasmid. 1981 Jul;6(1):67–77. doi: 10.1016/0147-619x(81)90054-8. [DOI] [PubMed] [Google Scholar]
  38. Terzaghi B. E., Sandine W. E. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. doi: 10.1128/am.29.6.807-813.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Villafane R., Bechhofer D. H., Narayanan C. S., Dubnau D. Replication control genes of plasmid pE194. J Bacteriol. 1987 Oct;169(10):4822–4829. doi: 10.1128/jb.169.10.4822-4829.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Xu F. F., Pearce L. E., Yu P. L. Genetic analysis of a lactococcal plasmid replicon. Mol Gen Genet. 1991 May;227(1):33–39. doi: 10.1007/BF00260703. [DOI] [PubMed] [Google Scholar]
  41. te Riele H., Michel B., Ehrlich S. D. Single-stranded plasmid DNA in Bacillus subtilis and Staphylococcus aureus. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2541–2545. doi: 10.1073/pnas.83.8.2541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. van der Vossen J. M., van der Lelie D., Venema G. Isolation and characterization of Streptococcus cremoris Wg2-specific promoters. Appl Environ Microbiol. 1987 Oct;53(10):2452–2457. doi: 10.1128/aem.53.10.2452-2457.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES