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. 1992 Sep;174(17):5727–5731. doi: 10.1128/jb.174.17.5727-5731.1992

Isolation and characterization of Pseudomonas aeruginosa mutants blocked in the synthesis of pyoverdin.

P Visca 1, L Serino 1, N Orsi 1
PMCID: PMC206521  PMID: 1512205

Abstract

We have isolated and characterized by chemical and enzymatic analyses three distinct types of pyoverdin-defective (pvd) mutants of Pseudomonas aeruginosa PAO1. The pvd-1 mutant is an L-N5-hydroxyornithine (L-N5-OH-Orn) auxotroph unable to hydroxylate L-ornithine (L-Orn) in a cell-free system and requiring L-N5-OH-Orn for pyoverdin production. The other two types of mutants appear to be blocked in further steps of the biosynthetic pathway leading to pyoverdin, namely, the acylation of L-N5-OH-Orn (pvd-2) and chromophore synthesis (pvd-3). The different pvd mutations were all found to be located in the catA1 region at 47 min of the genetic map of P. aeruginosa PAO1.

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Selected References

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