Abstract
RNAs of Rickettsia prowazekii, an obligate intracytoplasmic bacterium, have been identified and analyzed by an RNase protection assay. Total RNA, a mixture of host cell RNA and rickettsial RNA, was isolated from rickettsia-infected mouse L929 cells by the hot-phenol method. After hybridization with specific antisense RNA probes and digestion with RNase, the protected products were analyzed by electrophoresis and autoradiography. The results show that there is only one mRNA species for the ATP/ADP translocase gene (tlc) but two mRNA species for the citrate synthase gene (gltA). RNA half-lives were determined by measuring the RNA remaining after addition of rifampin. The half-lives of tlc mRNA, gltA mRNA I, and gltA mRNA II in R. prowazekii are 8.4 +/- 0.6, 12.3 +/- 1.3, and 20.5 +/- 1.8 min, respectively. However, the half-lives of tlc mRNA and gltA mRNA I in recombinant Escherichia coli strains are 2.9 +/- 0.1 and 1.4 +/- 0.1 min, respectively. The 16S rRNA in R. prowazekii was also examined and shown to be stable.
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- Emory S. A., Belasco J. G. The ompA 5' untranslated RNA segment functions in Escherichia coli as a growth-rate-regulated mRNA stabilizer whose activity is unrelated to translational efficiency. J Bacteriol. 1990 Aug;172(8):4472–4481. doi: 10.1128/jb.172.8.4472-4481.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pang H., Winkler H. H. Copy number of the 16S rRNA gene in Rickettsia prowazekii. J Bacteriol. 1993 Jun;175(12):3893–3896. doi: 10.1128/jb.175.12.3893-3896.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pato M. L., Bennett P. M., von Meyenburg K. Messenger ribonucleic acid synthesis and degradation in Escherichia coli during inhibition of translation. J Bacteriol. 1973 Nov;116(2):710–718. doi: 10.1128/jb.116.2.710-718.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Turco J., Winkler H. H. Isolation of Rickettsia prowazekii with reduced sensitivity to gamma interferon. Infect Immun. 1989 Jun;57(6):1765–1772. doi: 10.1128/iai.57.6.1765-1772.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiss E. Growth and physiology of rickettsiae. Bacteriol Rev. 1973 Sep;37(3):259–283. doi: 10.1128/br.37.3.259-283.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williamson L. R., Plano G. V., Winkler H. H., Krause D. C., Wood D. O. Nucleotide sequence of the Rickettsia prowazekii ATP/ADP translocase-encoding gene. Gene. 1989 Aug 15;80(2):269–278. doi: 10.1016/0378-1119(89)90291-6. [DOI] [PubMed] [Google Scholar]
- Winkler H. H. Protein and RNA synthesis by isolated Rickettsia prowazekii. Infect Immun. 1987 Sep;55(9):2032–2036. doi: 10.1128/iai.55.9.2032-2036.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Winkler H. H. Rickettsia species (as organisms). Annu Rev Microbiol. 1990;44:131–153. doi: 10.1146/annurev.mi.44.100190.001023. [DOI] [PubMed] [Google Scholar]
- Wisseman C. L., Jr, Edlinger E. A., Waddell A. D., Jones M. R. Infection cycle of Rickettsia rickettsii in chicken embryo and L-929 cells in culture. Infect Immun. 1976 Oct;14(4):1052–1064. doi: 10.1128/iai.14.4.1052-1064.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wisseman C. L., Jr, Waddell A. D. In vitro studies on rickettsia-host cell interactions: intracellular growth cycle of virulent and attenuated Rickettsia prowazeki in chicken embryo cells in slide chamber cultures. Infect Immun. 1975 Jun;11(6):1391–1404. doi: 10.1128/iai.11.6.1391-1401.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wood D. O., Williamson L. R., Winkler H. H., Krause D. C. Nucleotide sequence of the Rickettsia prowazekii citrate synthase gene. J Bacteriol. 1987 Aug;169(8):3564–3572. doi: 10.1128/jb.169.8.3564-3572.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]