Abstract
Methanococcus vannielii is capable of degrading purines to the extent that each of these purines may serve as the sole nitrogen source for growth. Results presented here demonstrate that purine degradation by M. vannielii is accomplished by a route similar to that described for clostridia. Various characteristics of the purine-degrading pathway of M. vannielii are described. Additionally, it is shown that M. vannielii does not extensively degrade exogenously supplied guanine if that compound is present at levels near or lower than those required to supply the cellular guanine requirement. Under those conditions, M. vannielii incorporates the intact guanine molecule into its guanine nucleotide pool. The benefits of a purine-degrading pathway to methanogens are discussed.
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Selected References
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- Bowen T. L., Whitman W. B. Incorporation of Exogenous Purines and Pyrimidines by Methanococcus voltae and Isolation of Analog-Resistant Mutants. Appl Environ Microbiol. 1987 Aug;53(8):1822–1826. doi: 10.1128/aem.53.8.1822-1826.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DeMoll E., Tsai L. Conversion of purines to xanthine by Methanococcus vannielii. Arch Biochem Biophys. 1986 Nov 1;250(2):440–445. doi: 10.1016/0003-9861(86)90747-2. [DOI] [PubMed] [Google Scholar]
- DeMoll E., Tsai L. Utilization of purines or pyrimidines as the sole nitrogen source by Methanococcus vannielii. J Bacteriol. 1986 Aug;167(2):681–684. doi: 10.1128/jb.167.2.681-684.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eirich L. D., Vogels G. D., Wolfe R. S. Proposed structure for coenzyme F420 from Methanobacterium. Biochemistry. 1978 Oct 31;17(22):4583–4593. doi: 10.1021/bi00615a002. [DOI] [PubMed] [Google Scholar]
- Jaenchen R., Schönheit P., Thauer R. K. Studies on the biosynthesis of coenzyme F420 in methanogenic bacteria. Arch Microbiol. 1984 Apr;137(4):362–365. doi: 10.1007/BF00410735. [DOI] [PubMed] [Google Scholar]
- Jones W. J., Nagle D. P., Jr, Whitman W. B. Methanogens and the diversity of archaebacteria. Microbiol Rev. 1987 Mar;51(1):135–177. doi: 10.1128/mr.51.1.135-177.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mavrovouniotis M. L. Estimation of standard Gibbs energy changes of biotransformations. J Biol Chem. 1991 Aug 5;266(22):14440–14445. [PubMed] [Google Scholar]
- PRICER W. E., Jr, RABINOWITZ J. C. Purine fermentation by Clostridium cylindrosporum. V. Formiminoglycine. J Biol Chem. 1956 Oct;222(2):537–554. [PubMed] [Google Scholar]
- RABINOWITZ J. C., BARKER H. A. Purine fermentation by Clostridium cylindrosporum. I. Tracer experiments on the fermentation of guanine. J Biol Chem. 1956 Jan;218(1):147–160. [PubMed] [Google Scholar]
- RABINOWITZ J. C., PRICER W. E., Jr Purine fermentation by Clostridium cylindrosporum. IV. 4-Ureido-5-imidazolecarboxylic acid. J Biol Chem. 1956 Jan;218(1):189–199. [PubMed] [Google Scholar]
- RABINOWITZ J. C. Purine fermentation by Clostridium cylindrosporum. III. 4-Amino-5-imidazolecarboxylic acid and 4-aminoimidazole. J Biol Chem. 1956 Jan;218(1):175–187. [PubMed] [Google Scholar]
- Thauer R. K., Jungermann K., Decker K. Energy conservation in chemotrophic anaerobic bacteria. Bacteriol Rev. 1977 Mar;41(1):100–180. doi: 10.1128/br.41.1.100-180.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vogels G. D., Van der Drift C. Degradation of purines and pyrimidines by microorganisms. Bacteriol Rev. 1976 Jun;40(2):403–468. doi: 10.1128/br.40.2.403-468.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Worrell V. E., Nagle D. P., Jr Genetic and physiological characterization of the purine salvage pathway in the archaebacterium Methanobacterium thermoautotrophicum Marburg. J Bacteriol. 1990 Jun;172(6):3328–3334. doi: 10.1128/jb.172.6.3328-3334.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]