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. 1993 Sep;175(18):6046–6048. doi: 10.1128/jb.175.18.6046-6048.1993

The pSC101 par locus alters protein-DNA interactions in vivo at the plasmid replication origin.

H Ingmer 1, S N Cohen 1
PMCID: PMC206688  PMID: 8376350

Abstract

We report here direct evidence that mutations in the par locus affect protein-DNA interactions in vivo at the replication origin of plasmid pSC101. Concomitant with par-mediated plasmid stabilization, two sites in the origin region show an altered methylation pattern as detected by in vivo footprinting with dimethyl sulfate. One site is located near an integration host factor-binding sequence adjacent to the first of three direct repeats known to be involved in the initiation of pSC101 replication; the second site is within the third direct repeat.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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