Abstract
The desA gene of the cyanobacterium Synechocystis sp. strain PCC6803 was expressed in Escherichia coli, which does not contain any fatty acid desaturase. The product of the desA gene catalyzed the desaturation of fatty acids at the delta 12 position. This result demonstrates that desA is the structural gene for a delta 12 desaturase.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Esfahani M., Barnes E. M., Jr, Wakil S. J. Control of fatty acid composition in phospholipids of Escherichia coli: response to fatty acid supplements in a fatty acid auxotroph. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1057–1064. doi: 10.1073/pnas.64.3.1057. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoey T., Warrior R., Manak J., Levine M. DNA-binding activities of the Drosophila melanogaster even-skipped protein are mediated by its homeo domain and influenced by protein context. Mol Cell Biol. 1988 Nov;8(11):4598–4607. doi: 10.1128/mcb.8.11.4598. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Studier F. W., Rosenberg A. H., Dunn J. J., Dubendorff J. W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 1990;185:60–89. doi: 10.1016/0076-6879(90)85008-c. [DOI] [PubMed] [Google Scholar]
- Wada H., Gombos Z., Murata N. Enhancement of chilling tolerance of a cyanobacterium by genetic manipulation of fatty acid desaturation. Nature. 1990 Sep 13;347(6289):200–203. doi: 10.1038/347200a0. [DOI] [PubMed] [Google Scholar]
- Wada H., Schmidt H., Heinz E., Murata N. In vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes. J Bacteriol. 1993 Jan;175(2):544–547. doi: 10.1128/jb.175.2.544-547.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]