Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1993 Oct;175(19):6345–6347. doi: 10.1128/jb.175.19.6345-6347.1993

Evidence that the Rad1 and Rad10 proteins of Saccharomyces cerevisiae participate as a complex in nucleotide excision repair of UV radiation damage.

W Siede 1, A S Friedberg 1, E C Friedberg 1
PMCID: PMC206733  PMID: 8407807

Abstract

A newly characterized rad1 missense mutation (rad1-20) in the yeast Saccharomyces cerevisiae maps to a region of the Rad1 polypeptide known to be required for Rad1-Rad10 complex formation. The UV sensitivity of the rad1-20 mutant can be partially and specifically corrected by overexpression of wild-type Rad10 protein. These results suggest that complex formation between the Rad1 and Rad10 proteins is required for nucleotide excision repair.

Full text

PDF
6345

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailly V., Sommers C. H., Sung P., Prakash L., Prakash S. Specific complex formation between proteins encoded by the yeast DNA repair and recombination genes RAD1 and RAD10. Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8273–8277. doi: 10.1073/pnas.89.17.8273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bankmann M., Prakash L., Prakash S. Yeast RAD14 and human xeroderma pigmentosum group A DNA-repair genes encode homologous proteins. Nature. 1992 Feb 6;355(6360):555–558. doi: 10.1038/355555a0. [DOI] [PubMed] [Google Scholar]
  3. Bardwell A. J., Bardwell L., Johnson D. K., Friedberg E. C. Yeast DNA recombination and repair proteins Rad1 and Rad10 constitute a complex in vivo mediated by localized hydrophobic domains. Mol Microbiol. 1993 Jun;8(6):1177–1188. doi: 10.1111/j.1365-2958.1993.tb01662.x. [DOI] [PubMed] [Google Scholar]
  4. Bardwell L., Burtscher H., Weiss W. A., Nicolet C. M., Friedberg E. C. Characterization of the RAD10 gene of Saccharomyces cerevisiae and purification of Rad10 protein. Biochemistry. 1990 Mar 27;29(12):3119–3126. doi: 10.1021/bi00464a031. [DOI] [PubMed] [Google Scholar]
  5. Bardwell L., Cooper A. J., Friedberg E. C. Stable and specific association between the yeast recombination and DNA repair proteins RAD1 and RAD10 in vitro. Mol Cell Biol. 1992 Jul;12(7):3041–3049. doi: 10.1128/mcb.12.7.3041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fishman-Lobell J., Haber J. E. Removal of nonhomologous DNA ends in double-strand break recombination: the role of the yeast ultraviolet repair gene RAD1. Science. 1992 Oct 16;258(5081):480–484. doi: 10.1126/science.1411547. [DOI] [PubMed] [Google Scholar]
  7. Hoeijmakers J. H., Bootsma D. Molecular genetics of eukaryotic DNA excision repair. Cancer Cells. 1990 Oct;2(10):311–320. [PubMed] [Google Scholar]
  8. Klein H. L. Different types of recombination events are controlled by the RAD1 and RAD52 genes of Saccharomyces cerevisiae. Genetics. 1988 Oct;120(2):367–377. doi: 10.1093/genetics/120.2.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Naumovski L., Friedberg E. C. Molecular cloning of eucaryotic genes required for excision repair of UV-irradiated DNA: isolation and partial characterization of the RAD3 gene of Saccharomyces cerevisiae. J Bacteriol. 1982 Oct;152(1):323–331. doi: 10.1128/jb.152.1.323-331.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nicolet C. M., Friedberg E. C. Overexpression of the RAD2 gene of S. cerevisiae: identification and preliminary characterization of Rad2 protein. Yeast. 1987 Sep;3(3):149–160. doi: 10.1002/yea.320030303. [DOI] [PubMed] [Google Scholar]
  11. Park E., Guzder S. N., Koken M. H., Jaspers-Dekker I., Weeda G., Hoeijmakers J. H., Prakash S., Prakash L. RAD25 (SSL2), the yeast homolog of the human xeroderma pigmentosum group B DNA repair gene, is essential for viability. Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11416–11420. doi: 10.1073/pnas.89.23.11416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Prakash L. Defective thymine dimer excision in radiation-sensitive mutants rad10 and rad16 of Saccharomyces cerevisiae. Mol Gen Genet. 1977 Apr 29;152(3):125–128. doi: 10.1007/BF00268808. [DOI] [PubMed] [Google Scholar]
  13. Rose M. D., Broach J. R. Cloning genes by complementation in yeast. Methods Enzymol. 1991;194:195–230. doi: 10.1016/0076-6879(91)94017-7. [DOI] [PubMed] [Google Scholar]
  14. Rothstein R. Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. Methods Enzymol. 1991;194:281–301. doi: 10.1016/0076-6879(91)94022-5. [DOI] [PubMed] [Google Scholar]
  15. Schiestl R. H., Prakash S. RAD1, an excision repair gene of Saccharomyces cerevisiae, is also involved in recombination. Mol Cell Biol. 1988 Sep;8(9):3619–3626. doi: 10.1128/mcb.8.9.3619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schiestl R. H., Prakash S. RAD10, an excision repair gene of Saccharomyces cerevisiae, is involved in the RAD1 pathway of mitotic recombination. Mol Cell Biol. 1990 Jun;10(6):2485–2491. doi: 10.1128/mcb.10.6.2485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tomkinson A. E., Bardwell A. J., Bardwell L., Tappe N. J., Friedberg E. C. Yeast DNA repair and recombination proteins Rad1 and Rad10 constitute a single-stranded-DNA endonuclease. Nature. 1993 Apr 29;362(6423):860–862. doi: 10.1038/362860a0. [DOI] [PubMed] [Google Scholar]
  18. Van Houten B. Nucleotide excision repair in Escherichia coli. Microbiol Rev. 1990 Mar;54(1):18–51. doi: 10.1128/mr.54.1.18-51.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wang Z., Wu X., Friedberg E. C. Nucleotide-excision repair of DNA in cell-free extracts of the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):4907–4911. doi: 10.1073/pnas.90.11.4907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weiss W. A., Friedberg E. C. Molecular cloning and characterization of the yeast RAD10 gene and expression of RAD10 protein in E. coli. EMBO J. 1985 Jun;4(6):1575–1582. doi: 10.1002/j.1460-2075.1985.tb03819.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wilcox D. R., Prakash L. Incision and postincision steps of pyrimidine dimer removal in excision-defective mutants of Saccharomyces cerevisiae. J Bacteriol. 1981 Nov;148(2):618–623. doi: 10.1128/jb.148.2.618-623.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yang E., Friedberg E. C. Molecular cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD1 gene. Mol Cell Biol. 1984 Oct;4(10):2161–2169. doi: 10.1128/mcb.4.10.2161. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES