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. 1993 Oct;175(19):6354–6357. doi: 10.1128/jb.175.19.6354-6357.1993

Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene.

M M Vickerman 1, D G Heath 1, D B Clewell 1
PMCID: PMC206735  PMID: 8407809

Abstract

Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.

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Selected References

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