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. 1993 Oct;175(20):6717–6720. doi: 10.1128/jb.175.20.6717-6720.1993

Identification of an intracellular pyrimidine-specific endoribonuclease from Bacillus subtilis.

S Mathur 1, V J Cannistraro 1, D Kennell 1
PMCID: PMC206785  PMID: 8407848

Abstract

Two intracellular RNases which were easily separated by fractionation on strong anion- or cation-exchange resins were identified from Bacillus subtilis. One cleaved any phosphodiester bond, while the second cleaved only pyrimidine-N bonds. The enzyme with pyrimidine-N specificity was approximately 15 kDa, had a pH optimum of approximately 6.2, degraded C-C bonds approximately 10 times faster than U-U bonds, and was completely inactive against single-stranded DNA. The enzyme is called RNase C and may be the first reported broad-specificity endoribonuclease from B. subtilis.

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Selected References

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