Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1993 Oct;175(20):6721–6724. doi: 10.1128/jb.175.20.6721-6724.1993

Effects of ribosome-inactivating proteins on Escherichia coli and Agrobacterium tumefaciens translation systems.

T Girbés 1, L Barbieri 1, M Ferreras 1, F J Arias 1, M A Rojo 1, R Iglesias 1, C Alegre 1, C Escarmis 1, F Stirpe 1
PMCID: PMC206786  PMID: 8407849

Abstract

The effects of 30 type 1 and of 2 (ricin and volkensin) type 2 ribosome-inactivating proteins (RIPs) on Escherichia coli and Agrobacterium tumefaciens cell-free translation systems were compared with the effects on a rabbit reticulocyte translation system. The depurinating activity of RIPs on E. coli ribosomes was also evaluated. Only six type 1 RIPs inhibited endogenous mRNA-directed translational activity of E. coli lysates, with submicromolar 50% inhibitory concentrations. Four RIPs had similar activities on poly(U)-directed phenylalanine polymerization by E. coli ribosomes, and three RIPs inhibited poly(U)-directed polyphenylalanine synthesis by A. tumefaciens ribosomes, with submicromolar 50% inhibitory concentrations.

Full text

PDF
6721

Images in this article

6723Image
on p.6723

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barbieri L., Ferreras J. M., Barraco A., Ricci P., Stirpe F. Some ribosome-inactivating proteins depurinate ribosomal RNA at multiple sites. Biochem J. 1992 Aug 15;286(Pt 1):1–4. doi: 10.1042/bj2860001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cammarano P., Teichner A., Londei P., Acca M., Nicolaus B., Sanz J. L., Amils R. Insensitivity of archaebacterial ribosomes to protein synthesis inhibitors. Evolutionary implications. EMBO J. 1985 Mar;4(3):811–816. doi: 10.1002/j.1460-2075.1985.tb03702.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Endo Y., Chan Y. L., Lin A., Tsurugi K., Wool I. G. The cytotoxins alpha-sarcin and ricin retain their specificity when tested on a synthetic oligoribonucleotide (35-mer) that mimics a region of 28 S ribosomal ribonucleic acid. J Biol Chem. 1988 Jun 15;263(17):7917–7920. [PubMed] [Google Scholar]
  4. Endo Y., Mitsui K., Motizuki M., Tsurugi K. The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes. The site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins. J Biol Chem. 1987 Apr 25;262(12):5908–5912. [PubMed] [Google Scholar]
  5. Endo Y., Tsurugi K. The RNA N-glycosidase activity of ricin A-chain. The characteristics of the enzymatic activity of ricin A-chain with ribosomes and with rRNA. J Biol Chem. 1988 Jun 25;263(18):8735–8739. [PubMed] [Google Scholar]
  6. Escarmís C., Toja M., Medina M., Domingo E. Modifications of the 5' untranslated region of foot-and-mouth disease virus after prolonged persistence in cell culture. Virus Res. 1992 Nov;26(2):113–125. doi: 10.1016/0168-1702(92)90151-x. [DOI] [PubMed] [Google Scholar]
  7. Evensen G., Mathiesen A., Sundan A. Direct molecular cloning and expression of two distinct abrin A-chains. J Biol Chem. 1991 Apr 15;266(11):6848–6852. [PubMed] [Google Scholar]
  8. Girbés T., Cabrer B., Modolell J. Preparation and assay of purified Escherichia coli polysomes devoid of free ribosomal subunits and endogenous GTPase activities. Methods Enzymol. 1979;59:353–362. doi: 10.1016/0076-6879(79)59096-x. [DOI] [PubMed] [Google Scholar]
  9. Greco M., Montanaro L., Novello F., Saccone C., Sperti S., Stirpe P. Inhibition of protein synthesis by ricin: experiments with rat liver mitochondria and nuclei and with ribosomes from Escherichia coli. Biochem J. 1974 Sep;142(3):695–697. doi: 10.1042/bj1420695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Habuka N., Akiyama K., Tsuge H., Miyano M., Matsumoto T., Noma M. Expression and secretion of Mirabilis antiviral protein in Escherichia coli and its inhibition of in vitro eukaryotic and prokaryotic protein synthesis. J Biol Chem. 1990 Jul 5;265(19):10988–10992. [PubMed] [Google Scholar]
  11. Habuka N., Miyano M., Kataoka J., Noma M. Escherichia coli ribosome is inactivated by Mirabilis antiviral protein which cleaves the N-glycosidic bond at A2660 of 23 S ribosomal RNA. J Mol Biol. 1991 Oct 5;221(3):737–743. doi: 10.1016/0022-2836(91)80168-t. [DOI] [PubMed] [Google Scholar]
  12. Habuka N., Murakami Y., Noma M., Kudo T., Horikoshi K. Amino acid sequence of Mirabilis antiviral protein, total synthesis of its gene and expression in Escherichia coli. J Biol Chem. 1989 Apr 25;264(12):6629–6637. [PubMed] [Google Scholar]
  13. Hartley M. R., Legname G., Osborn R., Chen Z., Lord J. M. Single-chain ribosome inactivating proteins from plants depurinate Escherichia coli 23S ribosomal RNA. FEBS Lett. 1991 Sep 23;290(1-2):65–68. doi: 10.1016/0014-5793(91)81227-y. [DOI] [PubMed] [Google Scholar]
  14. Iglesias R., Escarmis C., Alegre C., Ferreras J. M., Girbés T. Fusidic acid-dependent ribosomal complexes protect Escherichia coli ribosomes from the action of the type 1 ribosome-inactivating protein crotin 2. FEBS Lett. 1993 Mar 1;318(2):189–192. doi: 10.1016/0014-5793(93)80019-q. [DOI] [PubMed] [Google Scholar]
  15. Lugnier A. A., Küntzel H., Dirheimer G. Inhibition of Neurospora crassa and yeast mitochondrial protein synthesis by ricin, a toxic protein inactive on E. coli protein synthesis. FEBS Lett. 1976 Jul 15;66(2):202–205. doi: 10.1016/0014-5793(76)80504-2. [DOI] [PubMed] [Google Scholar]
  16. McCann W. P., Hall L. M., Siler W., Barton N., Whitley R. J. High-pressure liquid chromatographic methods for determining arabinosyladenine-5'-monophosphate, arabinosyladenine, and arabinosylhypoxanthine in plasma and urine. Antimicrob Agents Chemother. 1985 Aug;28(2):265–273. doi: 10.1128/aac.28.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moazed D., Robertson J. M., Noller H. F. Interaction of elongation factors EF-G and EF-Tu with a conserved loop in 23S RNA. Nature. 1988 Jul 28;334(6180):362–364. doi: 10.1038/334362a0. [DOI] [PubMed] [Google Scholar]
  18. Modolell J., Davis B. D. Rapid inhibition of polypeptide chain extension by streptomycin. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1279–1286. doi: 10.1073/pnas.61.4.1279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nakatsu K., Tasker R. A., Bukowskyj M. Shortening of liquid chromatography columns for reduced retention time. Anal Chem. 1983 Jul;55(8):1455–1456. doi: 10.1021/ac00259a069. [DOI] [PubMed] [Google Scholar]
  20. Nicolson G. L., Blaustein J., Etzler M. E. Characterization of two plant lectins from Ricinus communis and their quantitative interaction with a murine lymphoma. Biochemistry. 1974 Jan 1;13(1):196–204. doi: 10.1021/bi00698a029. [DOI] [PubMed] [Google Scholar]
  21. O'Hare M., Roberts L. M., Thorpe P. E., Watson G. J., Prior B., Lord J. M. Expression of ricin A chain in Escherichia coli. FEBS Lett. 1987 May 25;216(1):73–78. doi: 10.1016/0014-5793(87)80759-7. [DOI] [PubMed] [Google Scholar]
  22. Prestle J., Hornung E., Schönfelder M., Mundry K. W. Mechanism and site of action of a ribosome-inactivating protein type 1 from Dianthus barbatus which inactivates Escherichia coli ribosomes. FEBS Lett. 1992 Feb 10;297(3):250–252. doi: 10.1016/0014-5793(92)80549-v. [DOI] [PubMed] [Google Scholar]
  23. Sallustio S., Stanley P. Isolation of Chinese hamster ovary ribosomal mutants differentially resistant to ricin, abrin, and modeccin. J Biol Chem. 1990 Jan 5;265(1):582–588. [PubMed] [Google Scholar]
  24. Saxena S. K., Ackerman E. J. Microinjected oligonucleotides complementary to the alpha-sarcin loop of 28 S RNA abolish protein synthesis in Xenopus oocytes. J Biol Chem. 1990 Feb 25;265(6):3263–3269. [PubMed] [Google Scholar]
  25. Shaw P. C., Yung M. H., Zhu R. H., Ho W. K., Ng T. B., Yeung H. W. Cloning of trichosanthin cDNA and its expression in Escherichia coli. Gene. 1991 Jan 15;97(2):267–272. doi: 10.1016/0378-1119(91)90061-f. [DOI] [PubMed] [Google Scholar]
  26. Sperti S., Brigotti M., Zamboni M., Carnicelli D., Montanaro L. Requirements for the inactivation of ribosomes by gelonin. Biochem J. 1991 Jul 1;277(Pt 1):281–284. doi: 10.1042/bj2770281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Stirpe F., Bailey S., Miller S. P., Bodley J. W. Modification of ribosomal RNA by ribosome-inactivating proteins from plants. Nucleic Acids Res. 1988 Feb 25;16(4):1349–1357. doi: 10.1093/nar/16.4.1349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Stirpe F., Barbieri L., Battelli M. G., Soria M., Lappi D. A. Ribosome-inactivating proteins from plants: present status and future prospects. Biotechnology (N Y) 1992 Apr;10(4):405–412. doi: 10.1038/nbt0492-405. [DOI] [PubMed] [Google Scholar]
  29. Stirpe F., Gasperi-Campani A., Barbieri L., Falasca A., Abbondanza A., Stevens W. A. Ribosome-inactivating proteins from the seeds of Saponaria officinalis L. (soapwort), of Agrostemma githago L. (corn cockle) and of Asparagus officinalis L. (asparagus), and from the latex of Hura crepitans L. (sandbox tree). Biochem J. 1983 Dec 15;216(3):617–625. doi: 10.1042/bj2160617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Stirpe F., Hughes R. C. Specificity of ribosome-inactivating proteins with RNA N-glycosidase activity. Biochem J. 1989 Sep 15;262(3):1001–1002. doi: 10.1042/bj2621001b. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Zamboni M., Brigotti M., Rambelli F., Montanaro L., Sperti S. High-pressure-liquid-chromatographic and fluorimetric methods for the determination of adenine released from ribosomes by ricin and gelonin. Biochem J. 1989 May 1;259(3):639–643. doi: 10.1042/bj2590639. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES