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. 1993 Nov;175(21):7081–7085. doi: 10.1128/jb.175.21.7081-7085.1993

Two overlapping genes encoding membrane proteins required for bacteriophage N4 adsorption.

D R Kiino 1, M S Singer 1, L B Rothman-Denes 1
PMCID: PMC206836  PMID: 8226649

Abstract

We present the nucleotide sequences of two genes whose products are required for bacteriophage N4 adsorption. The nfrA gene encodes a 122-kDa outer membrane protein which presumably serves as the phage receptor. The nfrB gene encodes an 85-kDa inner membrane protein and may be a component of the receptor.

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Selected References

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  1. Boulanger P., Letellier L. Characterization of ion channels involved in the penetration of phage T4 DNA into Escherichia coli cells. J Biol Chem. 1988 Jul 15;263(20):9767–9775. [PubMed] [Google Scholar]
  2. Boulanger P., Letellier L. Ion channels are likely to be involved in the two steps of phage T5 DNA penetration into Escherichia coli cells. J Biol Chem. 1992 Feb 15;267(5):3168–3172. [PubMed] [Google Scholar]
  3. Chou P. Y., Fasman G. D. Conformational parameters for amino acids in helical, beta-sheet, and random coil regions calculated from proteins. Biochemistry. 1974 Jan 15;13(2):211–222. doi: 10.1021/bi00699a001. [DOI] [PubMed] [Google Scholar]
  4. Chou P. Y., Fasman G. D. Prediction of protein conformation. Biochemistry. 1974 Jan 15;13(2):222–245. doi: 10.1021/bi00699a002. [DOI] [PubMed] [Google Scholar]
  5. Cozens B., Reithmeier R. A. Size and shape of rabbit skeletal muscle calsequestrin. J Biol Chem. 1984 May 25;259(10):6248–6252. [PubMed] [Google Scholar]
  6. Garnier J., Osguthorpe D. J., Robson B. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J Mol Biol. 1978 Mar 25;120(1):97–120. doi: 10.1016/0022-2836(78)90297-8. [DOI] [PubMed] [Google Scholar]
  7. Glaser-Wuttke G., Keppner J., Rasched I. Pore-forming properties of the adsorption protein of filamentous phage fd. Biochim Biophys Acta. 1989 Nov 3;985(3):239–247. doi: 10.1016/0005-2736(89)90408-2. [DOI] [PubMed] [Google Scholar]
  8. Graceffa P., Jancsó A., Mabuchi K. Modification of acidic residues normalizes sodium dodecyl sulfate-polyacrylamide gel electrophoresis of caldesmon and other proteins that migrate anomalously. Arch Biochem Biophys. 1992 Aug 15;297(1):46–51. doi: 10.1016/0003-9861(92)90639-e. [DOI] [PubMed] [Google Scholar]
  9. Guihard G., Boulanger P., Letellier L. Involvement of phage T5 tail proteins and contact sites between the outer and inner membrane of Escherichia coli in phage T5 DNA injection. J Biol Chem. 1992 Feb 15;267(5):3173–3178. [PubMed] [Google Scholar]
  10. Kiino D. R., Licudine R., Wilt K., Yang D. H., Rothman-Denes L. B. A cytoplasmic protein, NfrC, is required for bacteriophage N4 adsorption. J Bacteriol. 1993 Nov;175(21):7074–7080. doi: 10.1128/jb.175.21.7074-7080.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kiino D. R., Rothman-Denes L. B. Genetic analysis of bacteriophage N4 adsorption. J Bacteriol. 1989 Sep;171(9):4595–4602. doi: 10.1128/jb.171.9.4595-4602.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  13. Levengood-Freyermuth S. K., Click E. M., Webster R. E. Role of the carboxyl-terminal domain of TolA in protein import and integrity of the outer membrane. J Bacteriol. 1993 Jan;175(1):222–228. doi: 10.1128/jb.175.1.222-228.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pugsley A. P. The complete general secretory pathway in gram-negative bacteria. Microbiol Rev. 1993 Mar;57(1):50–108. doi: 10.1128/mr.57.1.50-108.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Robson B., Suzuki E. Conformational properties of amino acid residues in globular proteins. J Mol Biol. 1976 Nov 5;107(3):327–356. doi: 10.1016/s0022-2836(76)80008-3. [DOI] [PubMed] [Google Scholar]
  16. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shine J., Dalgarno L. Determinant of cistron specificity in bacterial ribosomes. Nature. 1975 Mar 6;254(5495):34–38. doi: 10.1038/254034a0. [DOI] [PubMed] [Google Scholar]
  18. Vogel H., Jähnig F. Models for the structure of outer-membrane proteins of Escherichia coli derived from raman spectroscopy and prediction methods. J Mol Biol. 1986 Jul 20;190(2):191–199. doi: 10.1016/0022-2836(86)90292-5. [DOI] [PubMed] [Google Scholar]
  19. Webster R. E. The tol gene products and the import of macromolecules into Escherichia coli. Mol Microbiol. 1991 May;5(5):1005–1011. doi: 10.1111/j.1365-2958.1991.tb01873.x. [DOI] [PubMed] [Google Scholar]
  20. Zagursky R. J., Berman M. L. Cloning vectors that yield high levels of single-stranded DNA for rapid DNA sequencing. Gene. 1984 Feb;27(2):183–191. doi: 10.1016/0378-1119(84)90139-2. [DOI] [PubMed] [Google Scholar]

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