Abstract
A 490-bp DNA segment spanning the junction between the ftsA and ftsZ genes inhibits cell division when present in high copy number. We show that this segment contains an antisense promoter and an antisense transcription terminator which define a new gene, stfZ.
Full text
PDF![7097](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a41b/206839/02b8e2d31354/jbacter00063-0373.png)
![7098](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a41b/206839/1160ab03330f/jbacter00063-0374.png)
![7099](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a41b/206839/dbbe00903d51/jbacter00063-0375.png)
![7100](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a41b/206839/e35c141b6c70/jbacter00063-0376.png)
![7101](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a41b/206839/2d2d35e95cfd/jbacter00063-0377.png)
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bouché F., Bouché J. P. Genetic evidence that DicF, a second division inhibitor encoded by the Escherichia coli dicB operon, is probably RNA. Mol Microbiol. 1989 Jul;3(7):991–994. doi: 10.1111/j.1365-2958.1989.tb00249.x. [DOI] [PubMed] [Google Scholar]
- Béjar S., Bouché J. P. A new dispensable genetic locus of the terminus region involved in control of cell division in Escherichia coli. Mol Gen Genet. 1985;201(2):146–150. doi: 10.1007/BF00425651. [DOI] [PubMed] [Google Scholar]
- Cesareni G., Helmer-Citterich M., Castagnoli L. Control of ColE1 plasmid replication by antisense RNA. Trends Genet. 1991 Jul;7(7):230–235. doi: 10.1016/0168-9525(91)90370-6. [DOI] [PubMed] [Google Scholar]
- Dai K., Lutkenhaus J. The proper ratio of FtsZ to FtsA is required for cell division to occur in Escherichia coli. J Bacteriol. 1992 Oct;174(19):6145–6151. doi: 10.1128/jb.174.19.6145-6151.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dewar S. J., Begg K. J., Donachie W. D. Inhibition of cell division initiation by an imbalance in the ratio of FtsA to FtsZ. J Bacteriol. 1992 Oct;174(19):6314–6316. doi: 10.1128/jb.174.19.6314-6316.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dewar S. J., Kagan-Zur V., Begg K. J., Donachie W. D. Transcriptional regulation of cell division genes in Escherichia coli. Mol Microbiol. 1989 Oct;3(10):1371–1377. doi: 10.1111/j.1365-2958.1989.tb00118.x. [DOI] [PubMed] [Google Scholar]
- Faubladier M., Cam K., Bouché J. P. Escherichia coli cell division inhibitor DicF-RNA of the dicB operon. Evidence for its generation in vivo by transcription termination and by RNase III and RNase E-dependent processing. J Mol Biol. 1990 Apr 5;212(3):461–471. doi: 10.1016/0022-2836(90)90325-G. [DOI] [PubMed] [Google Scholar]
- Robinson A. C., Kenan D. J., Hatfull G. F., Sullivan N. F., Spiegelberg R., Donachie W. D. DNA sequence and transcriptional organization of essential cell division genes ftsQ and ftsA of Escherichia coli: evidence for overlapping transcriptional units. J Bacteriol. 1984 Nov;160(2):546–555. doi: 10.1128/jb.160.2.546-555.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simons R. W., Kleckner N. Biological regulation by antisense RNA in prokaryotes. Annu Rev Genet. 1988;22:567–600. doi: 10.1146/annurev.ge.22.120188.003031. [DOI] [PubMed] [Google Scholar]
- Tétart F., Bouché J. P. Regulation of the expression of the cell-cycle gene ftsZ by DicF antisense RNA. Division does not require a fixed number of FtsZ molecules. Mol Microbiol. 1992 Mar;6(5):615–620. doi: 10.1111/j.1365-2958.1992.tb01508.x. [DOI] [PubMed] [Google Scholar]
- Wang H. C., Gayda R. C. High-level expression of the FtsA protein inhibits cell septation in Escherichia coli K-12. J Bacteriol. 1990 Aug;172(8):4736–4740. doi: 10.1128/jb.172.8.4736-4740.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wright J. J., Kumar A., Hayward R. S. Hypersymmetry in a transcriptional terminator of Escherichia coli confers increased efficiency as well as bidirectionality. EMBO J. 1992 May;11(5):1957–1964. doi: 10.1002/j.1460-2075.1992.tb05249.x. [DOI] [PMC free article] [PubMed] [Google Scholar]