Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1993 Nov;175(22):7471–7473. doi: 10.1128/jb.175.22.7471-7473.1993

Resistance of lambda cI translation to antibiotics that inhibit translation initiation.

K Chin 1, C S Shean 1, M E Gottesman 1
PMCID: PMC206893  PMID: 8226693

Abstract

The lambda cI lysogenic transcript is unusual in having no leader. Expression of a cI-lacZ protein fusion was relatively resistant to kasugamycin and pactamycin, which inhibit translation initiation on transcripts with leaders. Our data imply that there are distinct differences in translation initiation between the two classes of transcripts.

Full text

PDF
7471

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cam K. M., Oberto J., Weisberg R. A. The early promoters of bacteriophage HK022: contrasts and similarities to other lambdoid phages. J Bacteriol. 1991 Jan;173(2):734–740. doi: 10.1128/jb.173.2.734-740.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cohen L. B., Herner A. E., Goldberg I. H. Inhibition by pactamycin of the initiation of protein synthesis. Binding of N-acetylphenylalanyl transfer ribonucleic acid and polyuridylic acid to ribosomes. Biochemistry. 1969 Apr;8(4):1312–1326. doi: 10.1021/bi00832a004. [DOI] [PubMed] [Google Scholar]
  3. Faxén M., Plumbridge J., Isaksson L. A. Codon choice and potential complementarity between mRNA downstream of the initiation codon and bases 1471-1480 in 16S ribosomal RNA affects expression of glnS. Nucleic Acids Res. 1991 Oct 11;19(19):5247–5251. doi: 10.1093/nar/19.19.5247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gold L. Posttranscriptional regulatory mechanisms in Escherichia coli. Annu Rev Biochem. 1988;57:199–233. doi: 10.1146/annurev.bi.57.070188.001215. [DOI] [PubMed] [Google Scholar]
  5. Helser T. L., Davies J. E., Dahlberg J. E. Mechanism of kasugamycin resistance in Escherichia coli. Nat New Biol. 1972 Jan 5;235(53):6–9. doi: 10.1038/newbio235006a0. [DOI] [PubMed] [Google Scholar]
  6. Hui A., de Boer H. A. Specialized ribosome system: preferential translation of a single mRNA species by a subpopulation of mutated ribosomes in Escherichia coli. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4762–4766. doi: 10.1073/pnas.84.14.4762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ito K., Kawakami K., Nakamura Y. Multiple control of Escherichia coli lysyl-tRNA synthetase expression involves a transcriptional repressor and a translational enhancer element. Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):302–306. doi: 10.1073/pnas.90.1.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jacob W. F., Santer M., Dahlberg A. E. A single base change in the Shine-Dalgarno region of 16S rRNA of Escherichia coli affects translation of many proteins. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4757–4761. doi: 10.1073/pnas.84.14.4757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klock G., Hillen W. Expression, purification and operator binding of the transposon Tn1721-encoded Tet repressor. J Mol Biol. 1986 Jun 20;189(4):633–641. doi: 10.1016/0022-2836(86)90493-6. [DOI] [PubMed] [Google Scholar]
  10. Lodish H. G., Housman D., Jacobsen M. Initiation of hemoglobin synthesis. Specific inhibition by antibiotics and bacteriophage ribonucleic acid. Biochemistry. 1971 Jun 8;10(12):2348–2356. doi: 10.1021/bi00788a027. [DOI] [PubMed] [Google Scholar]
  11. Moazed D., Noller H. F. Binding of tRNA to the ribosomal A and P sites protects two distinct sets of nucleotides in 16 S rRNA. J Mol Biol. 1990 Jan 5;211(1):135–145. doi: 10.1016/0022-2836(90)90016-F. [DOI] [PubMed] [Google Scholar]
  12. Nagai H., Yuzawa H., Yura T. Interplay of two cis-acting mRNA regions in translational control of sigma 32 synthesis during the heat shock response of Escherichia coli. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10515–10519. doi: 10.1073/pnas.88.23.10515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Okuyama A., Machiyama N., Kinoshita T., Tanaka N. Inhibition by kasugamycin of initiation complex formation on 30S ribosomes. Biochem Biophys Res Commun. 1971 Apr 2;43(1):196–199. doi: 10.1016/s0006-291x(71)80106-7. [DOI] [PubMed] [Google Scholar]
  14. Okuyama A., Yoshikawa M., Tanaka N. Alteration of ribosomal protein S2 in kasugamycin-resistant mutant derived from Escherichia coli AB312. Biochem Biophys Res Commun. 1974 Oct 8;60(3):1163–1169. doi: 10.1016/0006-291x(74)90434-3. [DOI] [PubMed] [Google Scholar]
  15. Ptashne M., Backman K., Humayun M. Z., Jeffrey A., Maurer R., Meyer B., Sauer R. T. Autoregulation and function of a repressor in bacteriophage lambda. Science. 1976 Oct 8;194(4261):156–161. doi: 10.1126/science.959843. [DOI] [PubMed] [Google Scholar]
  16. Reichardt L., Kaiser A. D. Control of lambda repressor synthesis. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2185–2189. doi: 10.1073/pnas.68.9.2185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shean C. S., Gottesman M. E. Translation of the prophage lambda cl transcript. Cell. 1992 Aug 7;70(3):513–522. doi: 10.1016/0092-8674(92)90175-c. [DOI] [PubMed] [Google Scholar]
  18. Sprengart M. L., Fatscher H. P., Fuchs E. The initiation of translation in E. coli: apparent base pairing between the 16srRNA and downstream sequences of the mRNA. Nucleic Acids Res. 1990 Apr 11;18(7):1719–1723. doi: 10.1093/nar/18.7.1719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Steitz J. A. Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA. Nature. 1969 Dec 6;224(5223):957–964. doi: 10.1038/224957a0. [DOI] [PubMed] [Google Scholar]
  20. Stewart-Blair M. L., Yanowitz I. S., Goldberg I. H. Inhibition of synthesis of new globin chains in reticulocyte lysates by pactamycin. Biochemistry. 1971 Nov;10(23):4198–4206. doi: 10.1021/bi00799a007. [DOI] [PubMed] [Google Scholar]
  21. Tai P. C., Wallace B. J., Davis B. D. Actions of aurintricarboxylate, kasugamycin, and pactamycin on Escherichia coli polysomes. Biochemistry. 1973 Feb;12(4):616–620. doi: 10.1021/bi00728a008. [DOI] [PubMed] [Google Scholar]
  22. Tejedor F., Amils R., Ballesta J. P. Photoaffinity labeling of the pactamycin binding site on eubacterial ribosomes. Biochemistry. 1985 Jul 2;24(14):3667–3672. doi: 10.1021/bi00335a040. [DOI] [PubMed] [Google Scholar]
  23. Walz A., Pirrotta V., Ineichen K. Lambda repressor regulates the switch between PR and Prm promoters. Nature. 1976 Aug 19;262(5570):665–669. doi: 10.1038/262665a0. [DOI] [PubMed] [Google Scholar]
  24. Woodcock J., Moazed D., Cannon M., Davies J., Noller H. F. Interaction of antibiotics with A- and P-site-specific bases in 16S ribosomal RNA. EMBO J. 1991 Oct;10(10):3099–3103. doi: 10.1002/j.1460-2075.1991.tb07863.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yoshikawa M., Okuyama A., Tanaka N. A third kasugamycin resistance locus, ksgC, affecting ribosomal protein S2 in Escherichia coli K-12. J Bacteriol. 1975 May;122(2):796–797. doi: 10.1128/jb.122.2.796-797.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES