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. 1993 Nov;175(22):7500–7504. doi: 10.1128/jb.175.22.7500-7504.1993

Site-directed mutagenesis reveals the importance of conserved charged residues for the transport activity of the PheP permease of Escherichia coli.

J Pi 1, P J Wookey 1, A J Pittard 1
PMCID: PMC206900  PMID: 8226700

Abstract

Site-directed mutagenesis has been used to identify a number of charged residues essential for the transport activity of the PheP protein. These residues are highly conserved in the cluster of amino acid transporters. However, some other conserved residues and a number of aromatic residues have been shown not to be essential for transport activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES G. F. UPTAKE OF AMINO ACIDS BY SALMONELLA TYPHIMURIUM. Arch Biochem Biophys. 1964 Jan;104:1–18. doi: 10.1016/s0003-9861(64)80028-x. [DOI] [PubMed] [Google Scholar]
  2. Brown K. D. Formation of aromatic amino acid pools in Escherichia coli K-12. J Bacteriol. 1970 Oct;104(1):177–188. doi: 10.1128/jb.104.1.177-188.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Daniels D. L., Plunkett G., 3rd, Burland V., Blattner F. R. Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes. Science. 1992 Aug 7;257(5071):771–778. doi: 10.1126/science.1379743. [DOI] [PubMed] [Google Scholar]
  4. Deeley M. C., Yanofsky C. Nucleotide sequence of the structural gene for tryptophanase of Escherichia coli K-12. J Bacteriol. 1981 Sep;147(3):787–796. doi: 10.1128/jb.147.3.787-796.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dunten R. L., Sahin-Tóth M., Kaback H. R. Role of the charge pair aspartic acid-237-lysine-358 in the lactose permease of Escherichia coli. Biochemistry. 1993 Mar 30;32(12):3139–3145. doi: 10.1021/bi00063a028. [DOI] [PubMed] [Google Scholar]
  6. Hoffmann W. Molecular characterization of the CAN1 locus in Saccharomyces cerevisiae. A transmembrane protein without N-terminal hydrophobic signal sequence. J Biol Chem. 1985 Sep 25;260(21):11831–11837. [PubMed] [Google Scholar]
  7. Honoré N., Cole S. T. Nucleotide sequence of the aroP gene encoding the general aromatic amino acid transport protein of Escherichia coli K-12: homology with yeast transport proteins. Nucleic Acids Res. 1990 Feb 11;18(3):653–653. doi: 10.1093/nar/18.3.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jauniaux J. C., Grenson M. GAP1, the general amino acid permease gene of Saccharomyces cerevisiae. Nucleotide sequence, protein similarity with the other bakers yeast amino acid permeases, and nitrogen catabolite repression. Eur J Biochem. 1990 May 31;190(1):39–44. doi: 10.1111/j.1432-1033.1990.tb15542.x. [DOI] [PubMed] [Google Scholar]
  9. King S. C., Hansen C. L., Wilson T. H. The interaction between aspartic acid 237 and lysine 358 in the lactose carrier of Escherichia coli. Biochim Biophys Acta. 1991 Feb 25;1062(2):177–186. doi: 10.1016/0005-2736(91)90390-t. [DOI] [PubMed] [Google Scholar]
  10. Lee J. I., Hwang P. P., Hansen C., Wilson T. H. Possible salt bridges between transmembrane alpha-helices of the lactose carrier of Escherichia coli. J Biol Chem. 1992 Oct 15;267(29):20758–20764. [PubMed] [Google Scholar]
  11. Pi J., Wookey P. J., Pittard A. J. Cloning and sequencing of the pheP gene, which encodes the phenylalanine-specific transport system of Escherichia coli. J Bacteriol. 1991 Jun;173(12):3622–3629. doi: 10.1128/jb.173.12.3622-3629.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Reizer J., Finley K., Kakuda D., MacLeod C. L., Reizer A., Saier M. H., Jr Mammalian integral membrane receptors are homologous to facilitators and antiporters of yeast, fungi, and eubacteria. Protein Sci. 1993 Jan;2(1):20–30. doi: 10.1002/pro.5560020103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sahin-Tóth M., Dunten R. L., Gonzalez A., Kaback H. R. Functional interactions between putative intramembrane charged residues in the lactose permease of Escherichia coli. Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10547–10551. doi: 10.1073/pnas.89.21.10547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sarsero J. P., Wookey P. J., Gollnick P., Yanofsky C., Pittard A. J. A new family of integral membrane proteins involved in transport of aromatic amino acids in Escherichia coli. J Bacteriol. 1991 May;173(10):3231–3234. doi: 10.1128/jb.173.10.3231-3234.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Steffes C., Ellis J., Wu J., Rosen B. P. The lysP gene encodes the lysine-specific permease. J Bacteriol. 1992 May;174(10):3242–3249. doi: 10.1128/jb.174.10.3242-3249.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tanaka J., Fink G. R. The histidine permease gene (HIP1) of Saccharomyces cerevisiae. Gene. 1985;38(1-3):205–214. doi: 10.1016/0378-1119(85)90219-7. [DOI] [PubMed] [Google Scholar]
  18. Vandenbol M., Jauniaux J. C., Grenson M. Nucleotide sequence of the Saccharomyces cerevisiae PUT4 proline-permease-encoding gene: similarities between CAN1, HIP1 and PUT4 permeases. Gene. 1989 Nov 15;83(1):153–159. doi: 10.1016/0378-1119(89)90413-7. [DOI] [PubMed] [Google Scholar]
  19. Whipp M. J., Halsall D. M., Pittard A. J. Isolation and characterization of an Escherichia coli K-12 mutant defective in tyrosine- and phenylalanine-specific transport systems. J Bacteriol. 1980 Jul;143(1):1–7. doi: 10.1128/jb.143.1.1-7.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wookey P. J., Pittard A. J. DNA sequence of the gene (tyrP) encoding the tyrosine-specific transport system of Escherichia coli. J Bacteriol. 1988 Oct;170(10):4946–4949. doi: 10.1128/jb.170.10.4946-4949.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wookey P. J., Pittard J., Forrest S. M., Davidson B. E. Cloning of the tyrP gene and further characterization of the tyrosine-specific transport system in Escherichia coli K-12. J Bacteriol. 1984 Oct;160(1):169–174. doi: 10.1128/jb.160.1.169-174.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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