Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1993 Dec;175(23):7724–7726. doi: 10.1128/jb.175.23.7724-7726.1993

Mutational analysis of the bacteriophage P2 Ogr protein: truncation of the carboxy terminus.

K Gebhardt 1, R A King 1, G E Christie 1, B H Lindqvist 1
PMCID: PMC206935  PMID: 8244946

Abstract

The Ogr protein is a 72-residue, zinc-binding transcription factor essential for activation of late gene expression in bacteriophage P2. Analysis of C-terminal truncated proteins generated by stop codon mutagenesis shows that deletion of residues distal to position 51 had negligible effects on Ogr function. More-extensive deletion resulted in unstable products with severely reduced activity. These results, as well as the effects of other mutations in this region, support the idea that the 21 C-terminal residues are not required for transactivation.

Full text

PDF
7724

Images in this article

7725Image
on p.7725

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birkeland N. K., Christie G. E., Lindqvist B. H. Directed mutagenesis of the bacteriophage P2 ogr gene defines an essential function. Gene. 1988 Dec 20;73(2):327–335. doi: 10.1016/0378-1119(88)90497-0. [DOI] [PubMed] [Google Scholar]
  2. Birkeland N. K., Lindquist B. H. Coliphage P2 late control gene ogr. DNA sequence and product identification. J Mol Biol. 1986 Apr 5;188(3):487–490. doi: 10.1016/0022-2836(86)90170-1. [DOI] [PubMed] [Google Scholar]
  3. Christie G. E., Calendar R. Interactions between satellite bacteriophage P4 and its helpers. Annu Rev Genet. 1990;24:465–490. doi: 10.1146/annurev.ge.24.120190.002341. [DOI] [PubMed] [Google Scholar]
  4. Christie G. E., Haggård-Ljungquist E., Feiwell R., Calendar R. Regulation of bacteriophage P2 late-gene expression: the ogr gene. Proc Natl Acad Sci U S A. 1986 May;83(10):3238–3242. doi: 10.1073/pnas.83.10.3238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chung C. H., Goldberg A. L. The product of the lon (capR) gene in Escherichia coli is the ATP-dependent protease, protease La. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4931–4935. doi: 10.1073/pnas.78.8.4931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chung C. T., Niemela S. L., Miller R. H. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2172–2175. doi: 10.1073/pnas.86.7.2172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Coleman J. E. Zinc proteins: enzymes, storage proteins, transcription factors, and replication proteins. Annu Rev Biochem. 1992;61:897–946. doi: 10.1146/annurev.bi.61.070192.004341. [DOI] [PubMed] [Google Scholar]
  8. Fujiki H., Palm P., Zillig W., Calendar R., Sunshine M. Identification of a mutation within the structural gene for the a subunit of DNA-dependent RNA polymerase of E. coli. Mol Gen Genet. 1976 Apr 23;145(1):19–22. doi: 10.1007/BF00331552. [DOI] [PubMed] [Google Scholar]
  9. Grambow N. J., Birkeland N. K., Anders D. L., Christie G. E. Deletion analysis of a bacteriophage P2 late promoter. Gene. 1990 Oct 30;95(1):9–15. doi: 10.1016/0378-1119(90)90407-i. [DOI] [PubMed] [Google Scholar]
  10. Halling C., Sunshine M. G., Lane K. B., Six E. W., Calendar R. A mutation of the transactivation gene of satellite bacteriophage P4 that suppresses the rpoA109 mutation of Escherichia coli. J Bacteriol. 1990 Jul;172(7):3541–3548. doi: 10.1128/jb.172.7.3541-3548.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kalionis B., Pritchard M., Egan J. B. Control of gene expression in the P2-related temperate coliphages. IV. Concerning the late control gene and control of its transcription. J Mol Biol. 1986 Sep 20;191(2):211–220. doi: 10.1016/0022-2836(86)90258-5. [DOI] [PubMed] [Google Scholar]
  12. King R. A., Anders D. L., Christie G. E. Site-directed mutagenesis of an amino acid residue in the bacteriophage P2 ogr protein implicated in interaction with Escherichia coli RNA polymerase. Mol Microbiol. 1992 Nov;6(22):3313–3320. doi: 10.1111/j.1365-2958.1992.tb02199.x. [DOI] [PubMed] [Google Scholar]
  13. Kunkel T. A., Roberts J. D., Zakour R. A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 1987;154:367–382. doi: 10.1016/0076-6879(87)54085-x. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Lee T. C., Christie G. E. Purification and properties of the bacteriophage P2 ogr gene product. A prokaryotic zinc-binding transcriptional activator. J Biol Chem. 1990 May 5;265(13):7472–7477. [PubMed] [Google Scholar]
  16. Mitchell P. J., Tjian R. Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science. 1989 Jul 28;245(4916):371–378. doi: 10.1126/science.2667136. [DOI] [PubMed] [Google Scholar]
  17. Parsell D. A., Silber K. R., Sauer R. T. Carboxy-terminal determinants of intracellular protein degradation. Genes Dev. 1990 Feb;4(2):277–286. doi: 10.1101/gad.4.2.277. [DOI] [PubMed] [Google Scholar]
  18. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sasaki I., Bertani G. Growth abnormalities in Hfr derivatives of Escherichia coli strain C. J Gen Microbiol. 1965 Sep;40(3):365–376. doi: 10.1099/00221287-40-3-365. [DOI] [PubMed] [Google Scholar]
  20. Sun J., Inouye M., Inouye S. Association of a retroelement with a P4-like cryptic prophage (retronphage phi R73) integrated into the selenocystyl tRNA gene of Escherichia coli. J Bacteriol. 1991 Jul;173(13):4171–4181. doi: 10.1128/jb.173.13.4171-4181.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sunshine M. G., Sauer B. A bacterial mutation blocking P2 phage late gene expression. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2770–2774. doi: 10.1073/pnas.72.7.2770. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Van Bokkelen G. B., Dale E. C., Halling C., Calendar R. Mutational analysis of a bacteriophage P4 late promoter. J Bacteriol. 1991 Jan;173(1):37–45. doi: 10.1128/jb.173.1.37-45.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Vieira J., Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 1987;153:3–11. doi: 10.1016/0076-6879(87)53044-0. [DOI] [PubMed] [Google Scholar]
  24. Wiman M., Bertani G., Kelly B., Sasaki I. Genetic map of Escherichia coli strain C. Mol Gen Genet. 1970;107(1):1–31. doi: 10.1007/BF00433220. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES