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. 1993 Dec;175(24):8043–8048. doi: 10.1128/jb.175.24.8043-8048.1993

A hydroxylase-like gene product contributes to synthesis of a polyketide spore pigment in Streptomyces halstedii.

G Blanco 1, A Pereda 1, P Brian 1, C Méndez 1, K F Chater 1, J A Salas 1
PMCID: PMC206987  PMID: 8253693

Abstract

A gene, schC, adjacent to the sch gene cluster encoding the biosynthesis of a polyketide spore pigment in Streptomyces halstedii was sequenced. Its deduced product resembled flavin adenine nucleotide-containing hydroxylases involved in the biosynthesis of polycyclic aromatic polyketide antibiotics and in catabolic pathways of aromatic compounds. When schC was disrupted, the normally green spores of S. halstedii became lilac. An schC-like gene was located in an equivalent position next to a large gene cluster (whiE) known to determine spore pigment in Streptomyces coelicolor A3(2).

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  1. Blanco G., Brian P., Pereda A., Méndez C., Salas J. A., Chater K. F. Hybridization and DNA sequence analyses suggest an early evolutionary divergence of related biosynthetic gene sets encoding polyketide antibiotics and spore pigments in Streptomyces spp. Gene. 1993 Aug 16;130(1):107–116. doi: 10.1016/0378-1119(93)90352-4. [DOI] [PubMed] [Google Scholar]
  2. Blanco G., Pereda A., Méndez C., Salas J. A. Cloning and disruption of a fragment of Streptomyces halstedii DNA involved in the biosynthesis of a spore pigment. Gene. 1992 Mar 1;112(1):59–65. doi: 10.1016/0378-1119(92)90303-7. [DOI] [PubMed] [Google Scholar]
  3. Caballero J. L., Martinez E., Malpartida F., Hopwood D. A. Organisation and functions of the actVA region of the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor. Mol Gen Genet. 1991 Dec;230(3):401–412. doi: 10.1007/BF00280297. [DOI] [PubMed] [Google Scholar]
  4. Davis N. K., Chater K. F. Spore colour in Streptomyces coelicolor A3(2) involves the developmentally regulated synthesis of a compound biosynthetically related to polyketide antibiotics. Mol Microbiol. 1990 Oct;4(10):1679–1691. doi: 10.1111/j.1365-2958.1990.tb00545.x. [DOI] [PubMed] [Google Scholar]
  5. Decker H., Motamedi H., Hutchinson C. R. Nucleotide sequences and heterologous expression of tcmG and tcmP, biosynthetic genes for tetracenomycin C in Streptomyces glaucescens. J Bacteriol. 1993 Jun;175(12):3876–3886. doi: 10.1128/jb.175.12.3876-3886.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eggink G., Engel H., Vriend G., Terpstra P., Witholt B. Rubredoxin reductase of Pseudomonas oleovorans. Structural relationship to other flavoprotein oxidoreductases based on one NAD and two FAD fingerprints. J Mol Biol. 1990 Mar 5;212(1):135–142. doi: 10.1016/0022-2836(90)90310-I. [DOI] [PubMed] [Google Scholar]
  8. Guilfoile P. G., Hutchinson C. R. A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin. Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8553–8557. doi: 10.1073/pnas.88.19.8553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Haydock S. F., Dowson J. A., Dhillon N., Roberts G. A., Cortes J., Leadlay P. F. Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases. Mol Gen Genet. 1991 Nov;230(1-2):120–128. doi: 10.1007/BF00290659. [DOI] [PubMed] [Google Scholar]
  10. Hohn B., Collins J. A small cosmid for efficient cloning of large DNA fragments. Gene. 1980 Nov;11(3-4):291–298. doi: 10.1016/0378-1119(80)90069-4. [DOI] [PubMed] [Google Scholar]
  11. Hopwood D. A., Sherman D. H. Molecular genetics of polyketides and its comparison to fatty acid biosynthesis. Annu Rev Genet. 1990;24:37–66. doi: 10.1146/annurev.ge.24.120190.000345. [DOI] [PubMed] [Google Scholar]
  12. Horinouchi S., Beppu T. Construction and application of a promoter-probe plasmid that allows chromogenic identification in Streptomyces lividans. J Bacteriol. 1985 Apr;162(1):406–412. doi: 10.1128/jb.162.1.406-412.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kälin M., Neujahr H. Y., Weissmahr R. N., Sejlitz T., Jöhl R., Fiechter A., Reiser J. Phenol hydroxylase from Trichosporon cutaneum: gene cloning, sequence analysis, and functional expression in Escherichia coli. J Bacteriol. 1992 Nov;174(22):7112–7120. doi: 10.1128/jb.174.22.7112-7120.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nebert D. W., Gonzalez F. J. P450 genes: structure, evolution, and regulation. Annu Rev Biochem. 1987;56:945–993. doi: 10.1146/annurev.bi.56.070187.004501. [DOI] [PubMed] [Google Scholar]
  15. Nurk A., Kasak L., Kivisaar M. Sequence of the gene (pheA) encoding phenol monooxygenase from Pseudomonas sp. EST1001: expression in Escherichia coli and Pseudomonas putida. Gene. 1991 Jun 15;102(1):13–18. doi: 10.1016/0378-1119(91)90531-f. [DOI] [PubMed] [Google Scholar]
  16. Omer C. A., Lenstra R., Litle P. J., Dean C., Tepperman J. M., Leto K. J., Romesser J. A., O'Keefe D. P. Genes for two herbicide-inducible cytochromes P-450 from Streptomyces griseolus. J Bacteriol. 1990 Jun;172(6):3335–3345. doi: 10.1128/jb.172.6.3335-3345.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Perkins E. J., Gordon M. P., Caceres O., Lurquin P. F. Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4. J Bacteriol. 1990 May;172(5):2351–2359. doi: 10.1128/jb.172.5.2351-2359.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Poulos T. L., Finzel B. C., Howard A. J. High-resolution crystal structure of cytochrome P450cam. J Mol Biol. 1987 Jun 5;195(3):687–700. doi: 10.1016/0022-2836(87)90190-2. [DOI] [PubMed] [Google Scholar]
  19. Russel M., Model P. Sequence of thioredoxin reductase from Escherichia coli. Relationship to other flavoprotein disulfide oxidoreductases. J Biol Chem. 1988 Jun 25;263(18):9015–9019. [PubMed] [Google Scholar]
  20. Stassi D., Donadio S., Staver M. J., Katz L. Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis. J Bacteriol. 1993 Jan;175(1):182–189. doi: 10.1128/jb.175.1.182-189.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Weber J. M., Leung J. O., Swanson S. J., Idler K. B., McAlpine J. B. An erythromycin derivative produced by targeted gene disruption in Saccharopolyspora erythraea. Science. 1991 Apr 5;252(5002):114–117. doi: 10.1126/science.2011746. [DOI] [PubMed] [Google Scholar]
  22. Weijer W. J., Hofsteenge J., Vereijken J. M., Jekel P. A., Beintema J. J. Primary structure of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Biochim Biophys Acta. 1982 Jun 4;704(2):385–388. doi: 10.1016/0167-4838(82)90170-4. [DOI] [PubMed] [Google Scholar]
  23. Wierenga R. K., Terpstra P., Hol W. G. Prediction of the occurrence of the ADP-binding beta alpha beta-fold in proteins, using an amino acid sequence fingerprint. J Mol Biol. 1986 Jan 5;187(1):101–107. doi: 10.1016/0022-2836(86)90409-2. [DOI] [PubMed] [Google Scholar]
  24. Wright F., Bibb M. J. Codon usage in the G+C-rich Streptomyces genome. Gene. 1992 Apr 1;113(1):55–65. doi: 10.1016/0378-1119(92)90669-g. [DOI] [PubMed] [Google Scholar]

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