Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1991 Jan;173(2):457–462. doi: 10.1128/jb.173.2.457-462.1991

Phosphatidylglyceroylalkylamine, a novel phosphoglycolipid precursor in Deinococcus radiodurans.

Y Huang 1, R Anderson 1
PMCID: PMC207033  PMID: 1987140

Abstract

We report here the structure of a previously uncharacterized phospholipid in the radiation-resistant bacterium Deinococcus radiodurans. This phospholipid, designated lipid 4, was shown by chemical analysis, HF hydrolysis, and nuclear magnetic resonance spectroscopy to be phosphatidylglyceroylalkylamine. Lipid 4 thus contains the unusual lipid constituents glyceric acid and alkylamines, which have previously been identified in two complex phosphoglycolipids from this organism. By [32P]phosphate pulse-chase labeling techniques, lipid 4 was shown to be the precursor of the complex phosphoglycolipids alpha-galactosyl- and alpha-N-acetylglucosaminylphosphatidylglyceroylalkylamine. While phosphatidylglyceroylalkylamine is rapidly biosynthesized from Pi, its subsequent glycosylation occurs much more slowly. Therefore, we conclude that the final glycosylation step is the rate-limiting event in the biosynthesis of the complex phosphoglycolipids alpha-galactosyl- and alpha-N-acetylglucosaminyl-phosphatidylglyceroylalkylamine.

Full text

PDF
457

Images in this article

458Image
on p.458
459Image
on p.459
460Image
on p.460
461Image
on p.461

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson R., Hansen K. Structure of a novel phosphoglycolipid from Deinococcus radiodurans. J Biol Chem. 1985 Oct 5;260(22):12219–12223. [PubMed] [Google Scholar]
  2. Baumeister W., Kübler O. Topographic study of the cell surface of micrococcus radiodurans. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5525–5528. doi: 10.1073/pnas.75.11.5525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carbonneau M. A., Melin A. M., Perromat A., Clerc M. The action of free radicals on Deinococcus radiodurans carotenoids. Arch Biochem Biophys. 1989 Nov 15;275(1):244–251. doi: 10.1016/0003-9861(89)90370-6. [DOI] [PubMed] [Google Scholar]
  4. De Rosa M., Gambacorta A., Gliozzi A. Structure, biosynthesis, and physicochemical properties of archaebacterial lipids. Microbiol Rev. 1986 Mar;50(1):70–80. doi: 10.1128/mr.50.1.70-80.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  6. Fischer W., Ishizuka I., Landgraf H. R., Herrmann J. Glycerophosphoryl diglucosyl diglyceride, a new phosphoglycolipid from Streptococci. Biochim Biophys Acta. 1973 Mar 8;296(3):527–545. doi: 10.1016/0005-2760(73)90113-6. [DOI] [PubMed] [Google Scholar]
  7. Huang Y., Anderson R. Structure of a novel glucosamine-containing phosphoglycolipid from Deinococcus radiodurans. J Biol Chem. 1989 Nov 5;264(31):18667–18672. [PubMed] [Google Scholar]
  8. Kates M. The phytanyl ether-linked polar lipids and isoprenoid neutral lipids of extremely halophilic bacteria. Prog Chem Fats Other Lipids. 1978;15(4):301–342. doi: 10.1016/0079-6832(77)90011-8. [DOI] [PubMed] [Google Scholar]
  9. Lancy P., Jr, Murray R. G. The envelope of Micrococcus radiodurans: isolation, purification, and preliminary analysis of the wall layers. Can J Microbiol. 1978 Feb;24(2):162–176. doi: 10.1139/m78-029. [DOI] [PubMed] [Google Scholar]
  10. MERRICK T. P., BRUCE A. K. RADIATION RESPONSE OF POTASSIUM EFFLUX IN MICROCOCCUS RADIODURANS AND SARCINA LUTEA. Radiat Res. 1965 Apr;24:612–618. [PubMed] [Google Scholar]
  11. MOSELEY B. E., SCHEIN A. H. RADIATION RESISTANCE AND DEOXYRIBONUCLEIC ACID BASE COMPOSITION OF MICROCOCCUS RADIODURANS. Nature. 1964 Sep 19;203:1298–1299. doi: 10.1038/2031298a0. [DOI] [PubMed] [Google Scholar]
  12. Murray K., Mundy A. R., Blackford H. N., Stephenson T. P. Transvesical phenolisation of the pelvic plexuses: a simple technique for the treatment of refractory detrusor instability and hyperreflexia. Urol Int. 1986;41(3):202–206. doi: 10.1159/000281198. [DOI] [PubMed] [Google Scholar]
  13. Rebeyrotte N., Rebeyrotte P., Maviel M. J., Montaudon D. Lipides et lipopolyosides de Micrococcus radiodurans. Ann Microbiol (Paris) 1979 Nov-Dec;130B(4):407–414. [PubMed] [Google Scholar]
  14. Rouser G., Fkeischer S., Yamamoto A. Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids. 1970 May;5(5):494–496. doi: 10.1007/BF02531316. [DOI] [PubMed] [Google Scholar]
  15. SETLOW J. K., DUGGAN D. E. THE RESISTANCE OF MICROCOCCUS RADIODURANS TO ULTRAVIOLET RADIATION. I. ULTRAVIOLET-INDUCED LESIONS IN THE CELL'S DNA. Biochim Biophys Acta. 1964 Aug 12;87:664–668. doi: 10.1016/0926-6550(64)90284-1. [DOI] [PubMed] [Google Scholar]
  16. Sanders S. W., Maxcy R. B. Isolation of radiation-resistant bacteria without exposure to irradiation. Appl Environ Microbiol. 1979 Sep;38(3):436–439. doi: 10.1128/aem.38.3.436-439.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schein A. H. The deoxyribonucleic acid of Micrococcus radiodurans. Biochem J. 1966 Dec;101(3):647–650. doi: 10.1042/bj1010647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schleifer K. H., Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev. 1972 Dec;36(4):407–477. doi: 10.1128/br.36.4.407-477.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sleytr U. B., Kocur M., Glauert A. M., Thornley M. J. A study by freeze-etching of the fine structure of Micrococcus radiodurans. Arch Mikrobiol. 1973 Dec 4;94(1):77–87. doi: 10.1007/BF00414079. [DOI] [PubMed] [Google Scholar]
  20. Thompson B. G., Anderson R., Murray R. G. Unusual polar lipids of Micrococcus radiodurans strain Sark. Can J Microbiol. 1980 Dec;26(12):1408–1411. doi: 10.1139/m80-234. [DOI] [PubMed] [Google Scholar]
  21. Thornley M. J., Horne R. W., Glauert A. M. The fine structure of Micrococcus radiodurans. Arch Mikrobiol. 1965 Jul 20;51(3):267–289. doi: 10.1007/BF00408143. [DOI] [PubMed] [Google Scholar]
  22. WORK E. AMINO ACIDS OF WALLS OF MICROCOCCUS RADIODURANS. Nature. 1964 Mar 14;201:1107–1109. doi: 10.1038/2011107a0. [DOI] [PubMed] [Google Scholar]
  23. Work E., Griffiths H. Morphology and chemistry of cell walls of Micrococcus radiodurans. J Bacteriol. 1968 Feb;95(2):641–657. doi: 10.1128/jb.95.2.641-657.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES