Abstract
A neutron small-angle scattering study was performed to determine the thickness and the scattering density profile of isolated peptidoglycan sacculi of Escherichia coli W7 in aqueous suspension (D2O). The maximum thickness (7 +/- 0.5 nm) of the sacculus from the exponential-phase cells was large enough to suggest the existence of a more-than-single-layered architecture. The experimental density profile across the thickness of the sacculus did not allow an unambiguous differentiation between a single-layered architecture characterized by completely extended peptide side chains projecting from the sugar strands or, alternatively, a partially triple layered structure. To resolve this ambiguity, sacculi were labeled with deuterated wall peptides. Comparison of the two experimental profiles indicated that the sacculus is more than single layered across its surface, with about 75 to 80% of its surface single layered and 20 to 25% triple layered.
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Selected References
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- Begg K. J., Donachie W. D. Cell shape and division in Escherichia coli: experiments with shape and division mutants. J Bacteriol. 1985 Aug;163(2):615–622. doi: 10.1128/jb.163.2.615-622.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beveridge T. J., Davies J. A. Cellular responses of Bacillus subtilis and Escherichia coli to the Gram stain. J Bacteriol. 1983 Nov;156(2):846–858. doi: 10.1128/jb.156.2.846-858.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Botta G. A., Park J. T. Evidence for involvement of penicillin-binding protein 3 in murein synthesis during septation but not during cell elongation. J Bacteriol. 1981 Jan;145(1):333–340. doi: 10.1128/jb.145.1.333-340.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Braun V., Gnirke H., Henning U., Rehn K. Model for the structure of the shape-maintaining layer of the Escherichia coli cell envelope. J Bacteriol. 1973 Jun;114(3):1264–1270. doi: 10.1128/jb.114.3.1264-1270.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burge R. E., Fowler A. G., Reaveley D. A. Structure of the peptidogylcan of bacterial cell walls. I. J Mol Biol. 1977 Dec 25;117(4):927–953. doi: 10.1016/s0022-2836(77)80006-5. [DOI] [PubMed] [Google Scholar]
- Burman L. G., Park J. T. Molecular model for elongation of the murein sacculus of Escherichia coli. Proc Natl Acad Sci U S A. 1984 Mar;81(6):1844–1848. doi: 10.1073/pnas.81.6.1844. [DOI] [PMC free article] [PubMed] [Google Scholar]
- De Petris S. Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers. J Ultrastruct Res. 1967 Jul;19(1):45–83. doi: 10.1016/s0022-5320(67)80059-5. [DOI] [PubMed] [Google Scholar]
- Glauner B., Höltje J. V., Schwarz U. The composition of the murein of Escherichia coli. J Biol Chem. 1988 Jul 25;263(21):10088–10095. [PubMed] [Google Scholar]
- Goodell E. W., Higgins C. F. Uptake of cell wall peptides by Salmonella typhimurium and Escherichia coli. J Bacteriol. 1987 Aug;169(8):3861–3865. doi: 10.1128/jb.169.8.3861-3865.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goodell E. W. Recycling of murein by Escherichia coli. J Bacteriol. 1985 Jul;163(1):305–310. doi: 10.1128/jb.163.1.305-310.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Helmstetter C. E. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):507–518. doi: 10.1016/0022-2836(68)90424-5. [DOI] [PubMed] [Google Scholar]
- Hobot J. A., Carlemalm E., Villiger W., Kellenberger E. Periplasmic gel: new concept resulting from the reinvestigation of bacterial cell envelope ultrastructure by new methods. J Bacteriol. 1984 Oct;160(1):143–152. doi: 10.1128/jb.160.1.143-152.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koch A. L., Burdett I. D. The variable T model for gram-negative morphology. J Gen Microbiol. 1984 Sep;130(9):2325–2338. doi: 10.1099/00221287-130-9-2325. [DOI] [PubMed] [Google Scholar]
- Labischinski H., Barnickel G., Bradaczek H., Giesbrecht P. On the secondary and tertiary structure of murein. Low and medium-angle X-ray evidence against chitin-based conformations of bacterial peptidoglycan. Eur J Biochem. 1979 Mar 15;95(1):147–155. doi: 10.1111/j.1432-1033.1979.tb12949.x. [DOI] [PubMed] [Google Scholar]
- Labischinski H., Barnickel G., Naumann D., Keller P. Conformational and topological aspects of the three-dimensional architecture of bacterial peptidoglycan. Ann Inst Pasteur Microbiol. 1985 Jan-Feb;136A(1):45–50. doi: 10.1016/s0769-2609(85)80020-x. [DOI] [PubMed] [Google Scholar]
- Leduc M., Fréhel C., Siegel E., Van Heijenoort J. Multilayered distribution of peptidoglycan in the periplasmic space of Escherichia coli. J Gen Microbiol. 1989 May;135(5):1243–1254. doi: 10.1099/00221287-135-5-1243. [DOI] [PubMed] [Google Scholar]
- MURRAY R. G., STEED P., ELSON H. E. THE LOCATION OF THE MUCOPEPTIDE IN SECTIONS OF THE CELL WALL OF ESCHERICHIA COLI AND OTHER GRAM-NEGATIVE BACTERIA. Can J Microbiol. 1965 Jun;11:547–560. doi: 10.1139/m65-072. [DOI] [PubMed] [Google Scholar]
- Schwarz U., Asmus A., Frank H. Autolytic enzymes and cell division of Escherichia coli. J Mol Biol. 1969 May 14;41(3):419–429. doi: 10.1016/0022-2836(69)90285-x. [DOI] [PubMed] [Google Scholar]
- Spratt B. G. Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2999–3003. doi: 10.1073/pnas.72.8.2999. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Torbet J., Norton M. Y. The structure of the cell wall of Staphylococcus aureus studied with neutron scattering and magnetic birefringence. FEBS Lett. 1982 Oct 18;147(2):201–206. doi: 10.1016/0014-5793(82)81042-9. [DOI] [PubMed] [Google Scholar]
- de Jonge B. L., Wientjes F. B., Jurida I., Driehuis F., Wouters J. T., Nanninga N. Peptidoglycan synthesis during the cell cycle of Escherichia coli: composition and mode of insertion. J Bacteriol. 1989 Nov;171(11):5783–5794. doi: 10.1128/jb.171.11.5783-5794.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]