Abstract
Fusobacterium nucleatum was grown in the presence of [14C]UDP. By means of sequential precipitation and chromatographic separation of the cytoplasmic content, a peptidoglycan [14C]UDP pentapeptide containing lanthionine was isolated. This finding indicates that lanthionine is synthesized and incorporated as such during the assembly of the peptidoglycan.
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Selected References
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- Dzink J. L., Socransky S. S., Haffajee A. D. The predominant cultivable microbiota of active and inactive lesions of destructive periodontal diseases. J Clin Periodontol. 1988 May;15(5):316–323. doi: 10.1111/j.1600-051x.1988.tb01590.x. [DOI] [PubMed] [Google Scholar]
- Flouret B., Mengin-Lecreulx D., van Heijenoort J. Reverse-phase high-pressure liquid chromatography of uridine diphosphate N-acetylmuramyl peptide precursors of bacterial cell wall peptidoglycan. Anal Biochem. 1981 Jun;114(1):59–63. doi: 10.1016/0003-2697(81)90451-6. [DOI] [PubMed] [Google Scholar]
- Mengin-Lecreulx D., Flouret B., van Heijenoort J. Cytoplasmic steps of peptidoglycan synthesis in Escherichia coli. J Bacteriol. 1982 Sep;151(3):1109–1117. doi: 10.1128/jb.151.3.1109-1117.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROSENTHAL S., SHARON N. THE USE OF SEPHADEX G-25 FOR THE ISOLATION OF NUCLEOTIDE SUGAR DERIVATIVES FROM MICROCOCCUS LYSODEIKTICUS. Biochim Biophys Acta. 1964 Nov 1;83:378–380. doi: 10.1016/0926-6526(64)90025-4. [DOI] [PubMed] [Google Scholar]
- Vasstrand E. N., Hofstad T., Endresen C., Jensen H. B. Demonstration of lanthionine as a natural constituent of the peptidoglycan of Fusobacterium nucleatum. Infect Immun. 1979 Sep;25(3):775–780. doi: 10.1128/iai.25.3.775-780.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vasstrand E. N., Jensen H. B., Miron T., Hofstad T. Composition of peptidoglycans in Bacteroidaceae: determination and distribution of lanthionine. Infect Immun. 1982 Apr;36(1):114–122. doi: 10.1128/iai.36.1.114-122.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vasstrand E. N. Lysozyme digestion and chemical characterization of the peptidoglycan of Fusobacterium nucleatum Fev 1. Infect Immun. 1981 Jul;33(1):75–82. doi: 10.1128/iai.33.1.75-82.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vasstrand E., Jensen H. B., Miron T. Microbore single-column analysis of amino acids and amino sugars specific to bacterial cell wall peptidoglycans. Anal Biochem. 1980 Jun;105(1):154–158. doi: 10.1016/0003-2697(80)90438-8. [DOI] [PubMed] [Google Scholar]
- Walsh R. G., Nashef A. S., Feeney R. E. Intramolecular cross-linking of proteins by formation of lysinoalanine or lanthionine. Modification of disulfides in ovomucoids. Int J Pept Protein Res. 1979 Oct;14(4):290–299. doi: 10.1111/j.1399-3011.1979.tb01935.x. [DOI] [PubMed] [Google Scholar]