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. 1982 Apr;75(4):613–616. doi: 10.1111/j.1476-5381.1982.tb09181.x

Hypothalamic catecholamines and tolerance for severe cold in ethanol-treated guinea-pigs.

P Huttunen
PMCID: PMC2071534  PMID: 7066609

Abstract

1 The effect of ethanol (2g/kg) on hypothalamic catecholamines in guinea-pigs kept at room temperature (20 degrees C) and in severe cold (-20 degrees C) for 1.5 h was determined. Serum glucose, triacyl-glycerols and free fatty acids (FFAs), glycogen in liver and skeletal muscle and total lipid and triacyglycerols in the interscapular adipose tissue were also determined. 2 Ethanol increase the noradrenaline and adrenaline content of the hypothalamus at 20 degrees C and reduced ther rectal temperature by about 2 degrees C. The hypothalamic noradrenaline content of the ethanol-treated guinea-pigs exposed to cold, in which the fall in rectal temperature was about 8 degrees C, was higher than in the controls, whose rectal temperature decreased only by about 2 degrees C. 3 Cold exposure increased FFA concentration in serum and reduced skeletal muscle glycogen and serum glucose concentrations in both groups, but no significant differences were found in the carbohydrate and lipid values between the groups at -20 degrees C. 4 It is possible that the diminished cold tolerance in the ethanol-treated guinea-pigs might be due, at least in part, to the effect of ethanol on the catecholamines in the hypothalamus.

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Selected References

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  1. ANDERSEN K. L., HELLSTROM B., LORENTZEN F. V. COMBINED EFFECT OF COLD AND ALCOHOL ON HEAT BALANCE IN MAN. J Appl Physiol. 1963 Sep;18:975–982. doi: 10.1152/jappl.1963.18.5.975. [DOI] [PubMed] [Google Scholar]
  2. Bacopoulos N. G., Bhatnagar R. K., Van Orden L. S. The effects of subhypnotic doses of ethanol on regional catecholamine turnover. J Pharmacol Exp Ther. 1978 Jan;204(1):1–10. [PubMed] [Google Scholar]
  3. Corrodi H., Fuxe K., Hökfelt T. The effect of ethanol on the activity of central catecholamine neurones in rat brain. J Pharm Pharmacol. 1966 Dec;18(12):821–823. doi: 10.1111/j.2042-7158.1966.tb07817.x. [DOI] [PubMed] [Google Scholar]
  4. FELDBERG W., MYERS R. D. A NEW CONCEPT OF TEMPERATURE REGULATION BY AMINES IN THE HYPOTHALAMUS. Nature. 1963 Dec 28;200:1325–1325. doi: 10.1038/2001325a0. [DOI] [PubMed] [Google Scholar]
  5. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  6. GURSEY D., OLSON R. E. Depression of serotonin and norepinephrine levels in brain stem of rabbit by ethanol. Proc Soc Exp Biol Med. 1960 Jun;104:280–281. doi: 10.3181/00379727-104-25807. [DOI] [PubMed] [Google Scholar]
  7. HAGGENDAL J., LINDQVIST M. In effectiveness of ethanol on noradrenaline, dopamine or 5-hydroxytryptamine levels in brain. Acta Pharmacol Toxicol (Copenh) 1961;18:278–280. doi: 10.1111/j.1600-0773.1961.tb01668.x. [DOI] [PubMed] [Google Scholar]
  8. Himms-Hagen J. Lipid metabolism during cold-exposure and during cold-acclimation. Lipids. 1972 May;7(5):310–323. doi: 10.1007/BF02532649. [DOI] [PubMed] [Google Scholar]
  9. Huttunen P., Hirvonen J. The effect of ethanol on the ability of guinea-pigs to survive severe cold. Forensic Sci. 1977 May-Jun;9(3):185–193. doi: 10.1016/0300-9432(77)90090-5. [DOI] [PubMed] [Google Scholar]
  10. Huttunen P., Penttinen J., Hirvonen J. The effect of ethanol and cold-adaptation on the survival of guinea pigs in severe cold. Z Rechtsmed. 1980;85(4):289–294. doi: 10.1007/BF00201292. [DOI] [PubMed] [Google Scholar]
  11. Laurell S., Tibbling G. Colorimetric micro-determination of free fatty acids in plasma. Clin Chim Acta. 1967 Apr;16(1):57–62. doi: 10.1016/0009-8981(67)90269-0. [DOI] [PubMed] [Google Scholar]
  12. Lo S., Russell J. C., Taylor A. W. Determination of glycogen in small tissue samples. J Appl Physiol. 1970 Feb;28(2):234–236. doi: 10.1152/jappl.1970.28.2.234. [DOI] [PubMed] [Google Scholar]
  13. Lomax P., Bajorek J. G., Chesarek W. A., Chaffee R. R. Ethanol-induced hypothermia in the rat. Pharmacology. 1980;21(4):288–294. doi: 10.1159/000137443. [DOI] [PubMed] [Google Scholar]
  14. Marzo A., Ghirardi P., Sardini D., Meroni G. Simplified measurement of monoglycerides, diglycerides, triglycerides, and free fatty acids in biological samples. Clin Chem. 1971 Mar;17(3):145–147. [PubMed] [Google Scholar]
  15. Pohorecky L. A. Effects of ethanol on central and peripheral noradrenergic neurons. J Pharmacol Exp Ther. 1974 May;189(2):380–391. [PubMed] [Google Scholar]
  16. Thadani P. V., Kulig B. M., Brown F. C., Beard J. D. Acute and chronic ethanol-induced alterations in brain norepinephrine metabolites in the rat. Biochem Pharmacol. 1976 Jan;25(1):93–94. doi: 10.1016/0006-2952(76)90177-5. [DOI] [PubMed] [Google Scholar]
  17. Thadani P. V., Truitt E. B., Jr Effect of acute ethanol or acetaldehyde administration on the uptake, release, metabolism and turnover rate of norepinephrine in rat brain. Biochem Pharmacol. 1977 Jun 15;26(12):1147–1150. doi: 10.1016/0006-2952(77)90059-4. [DOI] [PubMed] [Google Scholar]
  18. Yamanaka Y., Kono S. Brain catecholamine turnover in alcoholic mice. Hiroshima J Med Sci. 1974 Sep;23(2-3):105=13–105=13. [PubMed] [Google Scholar]
  19. Zeisberger E., Brück K. Alteration of shivering threshold in cold- and warm-adapted guinea pigs following intrahypothalamic injections of noradrenaline and of an adrenergic alpha-receptor blocking agent. Pflugers Arch. 1976 Mar 30;362(2):113–119. doi: 10.1007/BF00583636. [DOI] [PubMed] [Google Scholar]
  20. Zeisberger E., Brück K. Central effects of noradrenaline on the control of body temperature in the guinea-pig. Pflugers Arch. 1971;322(2):152–166. doi: 10.1007/BF00592295. [DOI] [PubMed] [Google Scholar]

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