Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1982 Mar;75(3):479–483. doi: 10.1111/j.1476-5381.1982.tb09164.x

beta 2-Adrenoceptors regulate induction of myocardial ornithine decarboxylase in mice in vivo.

J G Copeland, D F Larson, W R Roeske, D H Russell, J R Womble
PMCID: PMC2071574  PMID: 6121595

Abstract

The pharmacological characteristics of the myocardial adrenoceptor of the mouse have been examined during embryogenesis by measuring ornithine decarboxylase (ODC, EC 4.1.1.17) induction. 2 A four fold elevation of ODC activity was observed after isoprenaline (10 mg/kg, s.c.), and enzyme activity was increased two to three fold following adrenaline (1 mg/kg, s.c.) or terbutaline given by direct injection to the foetus (10 microgram/500 mg). 3 Pretreatment with the beta-adrenoceptor antagonist, propranolol (10 mg/kg), totally blocked the increase in ODC activity. 4 Elevation of myocardial ODC activity was not inhibited by metoprolol, a relatively specific beta-adrenoceptor antagonist, at a dose of 10 mg/kg. 5 Since the increase in ODC activity was blocked by a beta-adrenoceptor antagonist (propranolol) and enzyme activity was stimulated by terbutaline, a beta 2-agonist, we conclude that beta 2-adrenoceptors are selectively coupled to the regulation of murine cardiac ODC activity following catecholamine stimulation.

Full text

PDF
479

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ablad B., Borg K. O., Carlsson E., EK L., Johnson G., Malmfors T., Regårdh C. G. A survey of the pharmacological properties of metoprolol in animals and man. Acta Pharmacol Toxicol (Copenh) 1975;36(Suppl 5):7–23. doi: 10.1111/j.1600-0773.1975.tb03318.x. [DOI] [PubMed] [Google Scholar]
  2. Bareis D. L., Slotkin T. A. Responses of heart ornithine decarboxylase and adrenal catecholamines to methadone and sympathetic stimulants in developing and adults rats. J Pharmacol Exp Ther. 1978 Apr;205(1):164–174. [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Byus C. V., Chubb J. M., Huxtable R. J., Russell D. H. Increase in type I adenosine 3',5'-monophosphate-dependent protein kinase during isoproterenol-induced cardiac hypertrophy. Biochem Biophys Res Commun. 1976 Dec 6;73(3):694–702. doi: 10.1016/0006-291x(76)90866-4. [DOI] [PubMed] [Google Scholar]
  5. Conolly M. E., Kersting F., Dollery C. T. The clinical pharmacology of beta-adrenoceptor-blocking drugs. Prog Cardiovasc Dis. 1976 Nov-Dec;19(3):203–234. doi: 10.1016/0033-0620(76)90016-5. [DOI] [PubMed] [Google Scholar]
  6. Duell E. A. Identification of a beta 2-adrenergic receptor in mammalian epidermis. Biochem Pharmacol. 1980 Jan 1;29(1):97–101. doi: 10.1016/0006-2952(80)90250-6. [DOI] [PubMed] [Google Scholar]
  7. Feldman M. J., Russell D. H. Polyamine biogenesis in left ventricle of the rat heart after aortic constriction. Am J Physiol. 1972 May;222(5):1199–1203. doi: 10.1152/ajplegacy.1972.222.5.1199. [DOI] [PubMed] [Google Scholar]
  8. Fischer J. E., Horst W. D., Kopin I. J. Norepinephrine metabolism in hypertrophied rat hearts. Nature. 1965 Aug 28;207(5000):951–953. doi: 10.1038/207951a0. [DOI] [PubMed] [Google Scholar]
  9. Fitzgerald J. D. Perspectives in adrenergic beta-receptor blockade. Clin Pharmacol Ther. 1969 May-Jun;10(3):292–306. doi: 10.1002/cpt1969103292. [DOI] [PubMed] [Google Scholar]
  10. Fuller R. W., Hemrick S. K. Studies of noradrenergic influence on cardiac ornithine decarboxylase in rats. J Mol Cell Cardiol. 1978 Nov;10(11):1031–1036. doi: 10.1016/0022-2828(78)90399-1. [DOI] [PubMed] [Google Scholar]
  11. Galant S. P., Allred S. J. Demonstration of beta-2 adrenergic receptors of high coupling efficiency in human neutrophil sonicates. J Lab Clin Med. 1980 Jul;96(1):15–23. [PubMed] [Google Scholar]
  12. Haddox M. K., Womble J. R., Larson D. F., Roeske W. R., Russell D. H. Isoproterenol stimulation of ornithine decarboxylase blocked by propranolol during ontogeny of the murine heart. Mol Pharmacol. 1981 Sep;20(2):382–386. [PubMed] [Google Scholar]
  13. Hellenbrecht D., Lemmer B., Wiethold G., Grobecker H. Measurement of hydrophobicity, surface activity, local anaesthesia, and myocardial conduction velocity as quantitative parameters of the non-specific membrane affinity of nine -adrenergic blocking agents. Naunyn Schmiedebergs Arch Pharmacol. 1973;277(2):211–226. doi: 10.1007/BF00501161. [DOI] [PubMed] [Google Scholar]
  14. Johnsson G., Regårdh C. G., Sölvell L. Combined pharmacokinetic and pharmacodynammc studies in man of the adrenergic beta1-receptor antagonist metoprolol. Acta Pharmacol Toxicol (Copenh) 1975;36(Suppl 5):31–44. doi: 10.1111/j.1600-0773.1975.tb03320.x. [DOI] [PubMed] [Google Scholar]
  15. Kather H., Simon B. Effects of salbutamol and butoxamine on the human fat cell adenylate cyclase. Horm Metab Res. 1980 Dec;12(12):695–697. doi: 10.1055/s-2007-999234. [DOI] [PubMed] [Google Scholar]
  16. Keely S. L., Corbin J. D., Park C. R. Regulation of adenosine 3:5-monophosphate-dependent protein kinase. J Biol Chem. 1975 Jul 10;250(13):4832–4840. [PubMed] [Google Scholar]
  17. Keely S. L., Jr, Lincoln T. M., Corbin J. D. Interaction of acetylcholine and epinephrine on heart cyclic AMP-dependent protein kinase. Am J Physiol. 1978 Apr;234(4):H432–H438. doi: 10.1152/ajpheart.1978.234.4.H432. [DOI] [PubMed] [Google Scholar]
  18. Laks M. M., Morady F., Swan H. J. Myocardial hypertrophy produced by chronic infusion of subhypertensive doses of norepinephrine in the dog. Chest. 1973 Jul;64(1):75–78. doi: 10.1378/chest.64.1.75. [DOI] [PubMed] [Google Scholar]
  19. Lands A. M., Arnold A., McAuliff J. P., Luduena F. P., Brown T. G., Jr Differentiation of receptor systems activated by sympathomimetic amines. Nature. 1967 May 6;214(5088):597–598. doi: 10.1038/214597a0. [DOI] [PubMed] [Google Scholar]
  20. Mary P. L., Rao J. P. Type of adrenoceptors involved in induction by adrenaline of hepatic alkaline phosphatase in mice in vivo. Br J Pharmacol. 1981 Jan;72(1):8–9. doi: 10.1111/j.1476-5381.1981.tb09096.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Minneman K. P., Hegstrand L. R., Molinoff P. B. Simultaneous determination of beta-1 and beta-2-adrenergic receptors in tissues containing both receptor subtypes. Mol Pharmacol. 1979 Jul;16(1):34–46. [PubMed] [Google Scholar]
  22. Nair K. G., Cutilletta A. F., Zak R., Koide T., Rabinowitz M. Biochemical correlates of cardiac hypertrophy. I. Experimental model; changes in heart weight, RNA content, and nuclear RNA polymerase activity. Circ Res. 1968 Sep;23(3):451–462. doi: 10.1161/01.res.23.3.451. [DOI] [PubMed] [Google Scholar]
  23. Onaya T., Akasu F., Takazawa K., Hashizume K. Evidence for activation by beta2-adrenergic receptors of adenosine 3',5'-monophosphate formation in Ehrlich ascites tumor cells. Endocrinology. 1978 Oct;103(4):1122–1127. doi: 10.1210/endo-103-4-1122. [DOI] [PubMed] [Google Scholar]
  24. Osnes J., Oye I. Relationship between cyclic AMP metabolism and inotropic response of perfused rat hearts to phenylephrine and other adrenergic amines. Adv Cyclic Nucleotide Res. 1975;5:415–433. [PubMed] [Google Scholar]
  25. Rabinowitz B., Chuck L., Kligerman M., Parmley W. W. Positive inotropic effects of methoxamine: evidence for alpha-adrenergic receptors in ventricular myocardium. Am J Physiol. 1975 Sep;229(3):582–585. doi: 10.1152/ajplegacy.1975.229.3.582. [DOI] [PubMed] [Google Scholar]
  26. Russell D. H. Ornithine decarboxylase as a biological and pharmacological tool. Pharmacology. 1980;20(3):117–129. doi: 10.1159/000137355. [DOI] [PubMed] [Google Scholar]
  27. Russell D., Snyder S. H. Amine synthesis in rapidly growing tissues: ornithine decarboxylase activity in regenerating rat liver, chick embryo, and various tumors. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1420–1427. doi: 10.1073/pnas.60.4.1420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schümann H. J., Endoh S., Brodde O. E. The time course of the effects of beta- and alpha-adrenoceptor stimulation by isoprenaline and methoxamine on the contractile force and cAMP level of the isolated rabbit papillary muscle. Naunyn Schmiedebergs Arch Pharmacol. 1975;289(3):291–302. doi: 10.1007/BF00499982. [DOI] [PubMed] [Google Scholar]
  29. Schümann H. J., Motomura S., Endoh M., Brodde O. E. Comparison of the mechanisms underlying the positive inotropic actions of dopamine, adrenaline and isoprenaline on the isolated rabbit papillary muscle. Naunyn Schmiedebergs Arch Pharmacol. 1977 Apr;297(3):257–267. doi: 10.1007/BF00509270. [DOI] [PubMed] [Google Scholar]
  30. Stanton H. C., Brenner G., Mayfield E. D., Jr Studies on isoproterenol-induced cardiomegaly in rats. Am Heart J. 1969 Jan;77(1):72–80. doi: 10.1016/0002-8703(69)90131-8. [DOI] [PubMed] [Google Scholar]
  31. Veldhuis J. D., Harrison T. S., Hammond J. M. Beta-2-Adrenergic stimulation of ornithine decarboxylase activity in porcine granulosa cells in vitro. Biochim Biophys Acta. 1980 Jan 17;627(2):123–130. doi: 10.1016/0304-4165(80)90314-1. [DOI] [PubMed] [Google Scholar]
  32. Verma S. C., McNeill J. H. Biochemical and mechanical effects of phenylephrine on the heart. Eur J Pharmacol. 1976 Apr;36(2):447–450. doi: 10.1016/0014-2999(76)90100-x. [DOI] [PubMed] [Google Scholar]
  33. Warnica J. W., Antony P., Gibson K., Harris P. The effect of isoprenaline and propranolol on rat myocardial ornithine decarboxylase. Cardiovasc Res. 1975 Nov;9(6):793–796. doi: 10.1093/cvr/9.6.793. [DOI] [PubMed] [Google Scholar]
  34. Womble J. R., Haddox M. K., Russell D. H. Epinephrine elevation in plasma parallels canine cardiac hypertrophy. Life Sci. 1978 Nov 9;23(19):1951–1957. doi: 10.1016/0024-3205(78)90562-3. [DOI] [PubMed] [Google Scholar]
  35. Womble J. R., Larson D. F., Copeland J. G., Brown B. R., Haddox M. K., Russell D. H. Adrenal medulla denervation prevents stress-induced epinephrine plasma elevation and cardiac hypertrophy. Life Sci. 1980 Dec 15;27(24):2417–2420. doi: 10.1016/0024-3205(80)90513-5. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES