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. 1982 Feb;75(2):409–414. doi: 10.1111/j.1476-5381.1982.tb08802.x

Changes in body temperature and oxygen consumption rate of conscious mice produced by intrahypothalamic and intracerebroventricular injections of delta 9-tetrahydrocannabinol.

A G Fitton, R G Pertwee
PMCID: PMC2071615  PMID: 6313110

Abstract

delta 9-Tetrahydrocannabinol (delta 9-THC) was injected into the preoptic area of the anterior hypothalamus or into the third or fourth cerebral ventricle of the conscious mouse through a chronically implanted cannula and the effects on body temperature and oxygen consumption rate were measured. At an ambient temperature of 22 degrees C, injections of delta 9-THC into the fourth ventricle (5 and 10 microgram) produced dose-dependent falls in rectal temperature. Hypothermia was also observed after injections of the drug into the hypothalamus (5 and 10 microgram) or into the third ventricle (10 microgram). The hypothermia produced by delta 9-THC was associated with a fall in oxygen consumption rate. Falls in rectal temperature and in oxygen consumption rate were significantly greater after injection of delta 9-THC than after injection of the drug vehicle, Tween 80. The falls in rectal temperature and oxygen consumption rate produced by injection of delta 9-THC into the fourth ventricle were abolished by elevation of the ambient temperature from 22 to 32 degrees C. A pretreatment that consisted of subcutaneous injections of delta 9-THC (20 mg/kg) given once daily for three days produced tolerance to the hypothermic effect of the drug when injected on day 4 either into the fourth ventricle (10 microgram) or into a lateral tail vein (2.0 mg/kg). The results suggest that delta 9-THC acts centrally to alter thermoregulation in mice not only when it is injected directly into the hypothalamus or cerebral ventricles but also when it is given intravenously.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Christensen H. D., Freudenthal R. I., Gidley J. T., Rosenfeld R., Boegli G., Testino L., Brine D. R., Pitt C. G., Wall M. E. Activity of delta8- and delta9-tetrahydrocannabinol and related compounds in the mouse. Science. 1971 Apr 9;172(3979):165–167. doi: 10.1126/science.172.3979.165. [DOI] [PubMed] [Google Scholar]
  2. Dascombe M. J., Milton A. S. The effects of cyclic adenosine 3',5'-monophosphate and other adenine nucleotides on body temperature. J Physiol. 1975 Aug;250(1):143–160. doi: 10.1113/jphysiol.1975.sp011046. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gill E. W., Jones G. Brain levels of 1 -tetrahydrocannabinol and its metabolites in mice--correlation with behaviour, and the effect of the metabolic inhibitors SKF 525A and piperonyl butoxide. Biochem Pharmacol. 1972 Aug 15;21(16):2237–2248. doi: 10.1016/0006-2952(72)90039-1. [DOI] [PubMed] [Google Scholar]
  4. Gill E. W., Jones G., Lawrence D. K. Contribution of the metabolite 7-hydroxy-delta1-tetrahydrocannabinol towards the pharmacological activity of delta 1-tetrahydrocannabinol in mice. Biochem Pharmacol. 1973 Jan 15;22(2):175–184. doi: 10.1016/0006-2952(73)90271-2. [DOI] [PubMed] [Google Scholar]
  5. Gill E. W., Lawrence D. K. The distribution of delta1-tetrahydrocannabinol and 7-hydroxy-delta-tetrahydrocannabinol in the mouse brain after intraventricular injection. J Pharm Pharmacol. 1973 Dec;25(12):948–952. doi: 10.1111/j.2042-7158.1973.tb09984.x. [DOI] [PubMed] [Google Scholar]
  6. Gill E. W., Paton W. D., Pertwee R. G. Preliminary experiments on the chemistry and pharmacology of cannabis. Nature. 1970 Oct 10;228(5267):134–136. doi: 10.1038/228134a0. [DOI] [PubMed] [Google Scholar]
  7. Lawrence D. K., Pertwee R. G. Brain levels of delta1-tetrahydrocannabinol and its metabolites in mice tolerant to the hypothermic effect of delta1-tetrahydrocannabinol. Br J Pharmacol. 1973 Oct;49(2):373–375. doi: 10.1111/j.1476-5381.1973.tb08388.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nyemitei-Addo I., Pertwee R. G., Tavendale R. Stereotaxic implantation of cannulae for subsequent drug administration into the third ventricles of conscious mice [proceedings]. Br J Pharmacol. 1980 Jan;68(1):189P–189P. [PMC free article] [PubMed] [Google Scholar]
  9. Pertwee R. G., Tavendale R. Effects of delta9-tetrahydrocannabinol on the rates of oxygen consumption of mice. Br J Pharmacol. 1977 Aug;60(4):559–568. doi: 10.1111/j.1476-5381.1977.tb07535.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pertwee R. G. Tolerance to the effect of delta1-tetrahydrocannabinol on corticosterone levels in mouse plasma produced by repeated administration of cannabis extract or delta1-tetrahydrocannabinol. Br J Pharmacol. 1974 Jul;51(3):391–397. doi: 10.1111/j.1476-5381.1974.tb10674.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schmeling W. T., Hosko M. J. Hypothermia induced by delta9-tetrahydrocannabinol in rats with electrolytic lesions of preoptic region. Pharmacol Biochem Behav. 1976 Jul;5(1):79–83. doi: 10.1016/0091-3057(76)90291-4. [DOI] [PubMed] [Google Scholar]
  12. Schmeling W. T., Hosko M. J. Hypothermic effects of intraventricular and intravenous administration of cannabinoids in intact and brainstem transected cats. Neuropharmacology. 1980 Jun;19(6):567–573. doi: 10.1016/0028-3908(80)90028-3. [DOI] [PubMed] [Google Scholar]

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