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. 1981 Jul;73(3):791–796. doi: 10.1111/j.1476-5381.1981.tb16817.x

The effects of 4-aminopyridine on the isolated vas deferens and its effects on the inhibitory properties of adenosine, morphine, noradrenaline and gamma-aminobutyric acid.

T W Stone
PMCID: PMC2071713  PMID: 7248668

Abstract

1 Adenosine, adenosine 5'-triphosphate (ATP), morphine, noradrenaline, gamma-aminobutyric acid (GABA) phentolamine and amyl nitrite were used to inhibit electrically-evoked contractions of the isolated superfused vas deferens of the mouse. 2 The inhibitory effects of adenosine ATP, morphine, noradrenaline and GABA, which are thought to be due to presynaptic action, were reduced by perfusion with media containing 4-aminopyridine (4AP) or tetraethylammonium (TEA) ions. The inhibitory effects of phentolamine and amyl nitrite were unaffected by 4AP or TEA. 3 Quinidine, which like 4AP and TEA produced some increase to twitch height, did not reduce responses to the various agonists, indicating that an increased muscle contraction was not itself responsible for the reduced responses. 4 It is concluded that antagonism between 4AP and adenosine is not a specific interaction, as had been suggested, but probably reflects an interaction with Ca2+ requiring processes in the presynaptic terminal.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett M. R., Lavidis N. A. An electrophysiological analysis of the effects of morphine on the calcium dependence of neuromuscular transmission in the mouse vas deferens. Br J Pharmacol. 1980 Jun;69(2):185–191. doi: 10.1111/j.1476-5381.1980.tb07889.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bowery N. G., Doble A., Hill D. R., Hudson A. L., Shaw J. S., Turnbull M. J. Baclofen: a selective agonist for a novel type of GABA receptor proceedings. Br J Pharmacol. 1979 Nov;67(3):444P–445P. [PMC free article] [PubMed] [Google Scholar]
  3. Clanachan A. S., Johns A., Paton D. M. Presynaptic inhibitory actions of adenine nucleotides and adenosine on neurotransmission in the rat vas deferens. Neuroscience. 1977;2(4):597–602. doi: 10.1016/0306-4522(77)90056-2. [DOI] [PubMed] [Google Scholar]
  4. Drew G. M. The effect of different calcium concentrations on the inhibitory effect of presynaptic alpha-adrenoceptors in the rat vas deferens. Br J Pharmacol. 1978 Jul;63(3):417–419. doi: 10.1111/j.1476-5381.1978.tb07792.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Edstrom J. P., Phillis J. W. The effects of AMP on the potential of rat cerebral cortical neurones. Can J Physiol Pharmacol. 1976 Oct;54(5):787–790. doi: 10.1139/y76-110. [DOI] [PubMed] [Google Scholar]
  6. Henderson G., Hughes J., Kosterlitz H. W. A new example of a morphine-sensitive neuro-effector junction: adrenergic transmission in the mouse vas deferens. Br J Pharmacol. 1972 Dec;46(4):764–766. doi: 10.1111/j.1476-5381.1972.tb06901.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Henderson G., Hughes J. The effects of morphine on the release of noradrenaline from the mouse vas deferens. Br J Pharmacol. 1976 Aug;57(4):551–557. doi: 10.1111/j.1476-5381.1976.tb10384.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hermann A., Gorman A. L. External and internal effects of tetraethylammonium on voltage-dependent and Ca-dependent K+ currents components in molluscan pacemaker neurons. Neurosci Lett. 1979 Apr;12(1):87–92. doi: 10.1016/0304-3940(79)91485-x. [DOI] [PubMed] [Google Scholar]
  9. Horn A. S., Lambert J. J., Marshall I. G. A comparison of the facilitatory actions of 4-aminopyridine methiodide and 4-aminopyridine on neuromuscular transmission. Br J Pharmacol. 1979 Jan;65(1):53–62. doi: 10.1111/j.1476-5381.1979.tb17333.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Illes P., Thesleff S. 4-Aminopyridine and evoked transmitter release from motor nerve endings. Br J Pharmacol. 1978 Dec;64(4):623–629. doi: 10.1111/j.1476-5381.1978.tb17325.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jenkins D. A., Marshall I., Nasmyth P. A. An inhibitory role for noradrenaline in the mouse vas deferens. Br J Pharmacol. 1977 Dec;61(4):649–655. doi: 10.1111/j.1476-5381.1977.tb07558.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jones M. E., Spriggs T. L. Noradrenaline and motor transmission in the vas deferens of the mouse. Br J Pharmacol. 1975 Mar;53(3):323–331. [PMC free article] [PubMed] [Google Scholar]
  13. Llinás R., Walton K., Bohr V. Synaptic transmission in squid giant synapse after potassium conductance blockage with external 3- and 4-aminopyridine. Biophys J. 1976 Jan;16(1):83–86. doi: 10.1016/S0006-3495(76)85664-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marshall I., Nasmyth P. A., Nicholl C. G., Shepperson N. B. alpha-Adrenoceptors in the mouse vas deferens and their effects on its response to electrical stimulation. Br J Pharmacol. 1978 Jan;62(1):147–151. doi: 10.1111/j.1476-5381.1978.tb07018.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Meves H., Pichon Y. The effect of internal and external 4-aminopyridine on the potassium currents in intracellularly perfused squid giant axons. J Physiol. 1977 Jun;268(2):511–532. doi: 10.1113/jphysiol.1977.sp011869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Perkins M. N., Stone T. W. 4-Aminopyridine blockade of neuronal depressant responses to adenosine triphosphate. Br J Pharmacol. 1980 Nov;70(3):425–428. doi: 10.1111/j.1476-5381.1980.tb08720.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Phillis J. W., Kostopoulos G. K., Limacher J. J. Depression of corticospinal cells by various purines and pyrimidines. Can J Physiol Pharmacol. 1974 Dec;52(6):1226–1229. doi: 10.1139/y74-162. [DOI] [PubMed] [Google Scholar]
  18. Stone T. W. Physiological roles for adenosine and adenosine 5'-triphosphate in the nervous system. Neuroscience. 1981;6(4):523–555. doi: 10.1016/0306-4522(81)90145-7. [DOI] [PubMed] [Google Scholar]
  19. Stone T. W., Taylor D. A. Microiontophoretic studies of the effects of cylic nucleotides on excitability of neurones in the rat cerebral cortex. J Physiol. 1977 Apr;266(3):523–543. doi: 10.1113/jphysiol.1977.sp011780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Thesleff S. Aminopyridines and synaptic transmission. Neuroscience. 1980;5(8):1413–1419. doi: 10.1016/0306-4522(80)90002-0. [DOI] [PubMed] [Google Scholar]
  21. Thompson S. H. Three pharmacologically distinct potassium channels in molluscan neurones. J Physiol. 1977 Feb;265(2):465–488. doi: 10.1113/jphysiol.1977.sp011725. [DOI] [PMC free article] [PubMed] [Google Scholar]

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