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. 1982 Aug;76(4):595–602. doi: 10.1111/j.1476-5381.1982.tb09259.x

Inhibition by substance P of some peripheral actions of acetylcholine in the cat

SL Clark, RW Ryall
PMCID: PMC2071813  PMID: 6179558

Abstract

1 The effect of substance P on contractions of the nictitating membrane and pressor responses to acetylcholine (ACh) and dimethylphenyl-piperazinium (DMPP) which were mediated via nocotinic receptors was studied in cats anaesthetized with chloralose.

2 Substance P (2-20 nmol) injected into the lingual artery giving estimated concentrations in arterial blood of 10-6 to 10-5 M, or intravenously giving estimated concentrations in blood of 10-8 to 10-7 M, reduced hexamethonium-sensitive but not atropine-sensitive responses.

3 The pressor effects of ACh and DMPP injected intra-arterially in atropinized and non-atropinized cats respectively were consistently attenuated by substance P given intra-arterially or intravenously.

4 The contractile effect of ACh in atropinized and of DMPP in non-atropinized cats was attenuated by substance P injected intra-arterially but only rarely when the polypeptide was injected intravenously.

5 The depressor effects of substance P per se were variable in magnitude and duration as were the inhibitory effects upon nicotinic receptors. The depressor and inhibitory effects of substance P were unrelated.

6 There was desensitization to all of these effects of substance P which probably contributed to the variation in the magnitude of the effects observed.

7 Substance P had no effect on muscarinic actions of acetyl-β-methylcholine on the nictitating membrane or blood pressure.

8 The results are discussed in relation to the ubiquity of the modulatory actions of substance P on nicotinic receptors and in relation to the possible physiological significance of the action.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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