Abstract
1 The effects of three methylene analogues of adenosine 5'-triphosphate (ATP) or 5'-diphosphate (ADP) have been studied on insulin secretion from the isolated perfused pancreas of the rat: 5'-adenylmethylene diphosphonate or beta, gamma-methylene ATP, adenosine 5'-alpha, beta-methylene triphosphate or alpha, beta-methylene ATP and adenosine 5'-alpha, beta-methylene diphosphonate or alpha, beta-methylene ADP. 2 beta, gamma-Methylene ATP did not elicit any increase of insulin release: alpha, beta-methylene ATP and alpha, beta-methylene ADP induced a biphasic stimulation of insulin secretion; this effect was dose-related between 1.65 and 165 mumol/l. Relative potency ATP/alpha, beta-methylene ATP was 1.2 and ATP/alpha, beta-methylene ADP was 0.31. 3 Our results point to the importance of the steric and electronic characteristics of the polyphosphate chain of the analogues of ATP and ADP in inducing an insulin secretory effect. They support the hypothesis of a purine receptor for ATP and ADP.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Burnstock G. Purinergic receptors. J Theor Biol. 1976 Oct 21;62(2):491–503. doi: 10.1016/0022-5193(76)90133-8. [DOI] [PubMed] [Google Scholar]
- Feldman J. M., Jackson T. B. Specificity of nucleotide-induced insulin secretion. Endocrinology. 1974 Feb;94(2):388–394. doi: 10.1210/endo-94-2-388. [DOI] [PubMed] [Google Scholar]
- Flodgaard H., Torp-Pedersen C. A calcium ion-dependent adenosine triphosphate pyrophosphohydrolase in plasma membrane from rat liver. Demonstration that the adenosine triphosphate analogues adenosine 5'-[betagamma-imido]triphosphate and adenosine 5'-[betagamma-methylene]-triphosphate are substrates for the enzyme. Biochem J. 1978 Jun 1;171(3):817–820. doi: 10.1042/bj1710817. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HALES C. N., RANDLE P. J. Immunoassay of insulin with insulin-antibody precipitate. Biochem J. 1963 Jul;88:137–146. doi: 10.1042/bj0880137. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loubatieres-Mariani M. M., Chapal J., Lignon F., Valette G. Structural specificity of nucleotides for insulin secretory action from the isolated perfused rat pancreas. Eur J Pharmacol. 1979 Nov 16;59(3-4):277–286. doi: 10.1016/0014-2999(79)90291-7. [DOI] [PubMed] [Google Scholar]
- Loubatières A., Mariani M. M., Ribes G., de Malbosc H., Chapal J. Etude expérimentale d'un nouveau sulfamide hypoglycémiant particulièrement actif, le HB 419 ou glibenclamide. Diabetologia. 1969 Feb;5(1):1–10. doi: 10.1007/BF01212212. [DOI] [PubMed] [Google Scholar]
- Loubatiéres A. L., Loubatiéres-Mariani M. M., Chapal J. Adénosine triphosphate (ATP), adénosine 3'5' monophosphate syclique (3'5' AMP c) et sécrétion d'insuline. C R Seances Soc Biol Fil. 1972;166(12):1742–1746. [PubMed] [Google Scholar]
- MOOS C., ALPERT N. R., MYERS T. C. Effects of a phosphonic acid analog of adenosine triphosphate on actomyosin systems. Arch Biochem Biophys. 1960 Jun;88:183–192. doi: 10.1016/0003-9861(60)90220-4. [DOI] [PubMed] [Google Scholar]
- Satchell D. G., Maguire M. H. Inhibitory effects of adenine nucleotide analogs on the isolated guinea-pig taenia coli. J Pharmacol Exp Ther. 1975 Dec;195(3):540–548. [PubMed] [Google Scholar]
- Yount R. G. ATP analogs. Adv Enzymol Relat Areas Mol Biol. 1975;43:1–56. doi: 10.1002/9780470122884.ch1. [DOI] [PubMed] [Google Scholar]
- Yount R. G., Babcock D., Ballantyne W., Ojala D. Adenylyl imidodiphosphate, an adenosine triphosphate analog containing a P--N--P linkage. Biochemistry. 1971 Jun 22;10(13):2484–2489. doi: 10.1021/bi00789a009. [DOI] [PubMed] [Google Scholar]
- Yount R. G., Ojala D., Babcock D. Interaction of P--N--P and P--C--P analogs of adenosine triphosphate with heavy meromyosin, myosin, and actomyosin. Biochemistry. 1971 Jun 22;10(13):2490–2496. doi: 10.1021/bi00789a010. [DOI] [PubMed] [Google Scholar]