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. 1991 Jan;173(1):301–307. doi: 10.1128/jb.173.1.301-307.1991

Sequence domains required for the activity of avirulence genes avrB and avrC from Pseudomonas syringae pv. glycinea.

S J Tamaki 1, D Y Kobayashi 1, N T Keen 1
PMCID: PMC207187  PMID: 1987121

Abstract

avrB and avrC from Pseudomonas syringae pv. glycinea share significant amino acid homology but interact with different soybean resistance genes to elicit the hypersensitive defense reaction. Recombinant genes constructed between avrB and avrC revealed that the central regions were required for avirulence gene activity but the 5' and 3' termini were interchangeable. Recombinants involving the central regions did not yield any detectable avirulence gene activity, and no new avirulence phenotypes were observed from any of the chimeric genes. These results suggest that the protein products of avrB and avrC possess catalytic properties that are required for the avirulence phenotypes.

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Selected References

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  1. Huynh T. V., Dahlbeck D., Staskawicz B. J. Bacterial blight of soybean: regulation of a pathogen gene determining host cultivar specificity. Science. 1989 Sep 22;245(4924):1374–1377. doi: 10.1126/science.2781284. [DOI] [PubMed] [Google Scholar]
  2. KING E. O., WARD M. K., RANEY D. E. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954 Aug;44(2):301–307. [PubMed] [Google Scholar]
  3. Keen N. T., Tamaki S., Kobayashi D., Trollinger D. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene. 1988 Oct 15;70(1):191–197. doi: 10.1016/0378-1119(88)90117-5. [DOI] [PubMed] [Google Scholar]
  4. Knorr D. A., Dawson W. O. A point mutation in the tobacco mosaic virus capsid protein gene induces hypersensitivity in Nicotiana sylvestris. Proc Natl Acad Sci U S A. 1988 Jan;85(1):170–174. doi: 10.1073/pnas.85.1.170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kobayashi D. Y., Tamaki S. J., Keen N. T. Cloned avirulence genes from the tomato pathogen Pseudomonas syringae pv. tomato confer cultivar specificity on soybean. Proc Natl Acad Sci U S A. 1989 Jan;86(1):157–161. doi: 10.1073/pnas.86.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kobayashi D. Y., Tamaki S. J., Trollinger D. J., Gold S., Keen N. T. A gene from Pseudomonas syringae pv. glycinea with homology to avirulence gene D from P. s. pv. tomato but devoid of the avirulence phenotype. Mol Plant Microbe Interact. 1990 Mar-Apr;3(2):103–111. doi: 10.1094/mpmi-3-103. [DOI] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Spaink H. P., Weinman J., Djordjevic M. A., Wijffelman C. A., Okker R. J., Lugtenberg B. J. Genetic analysis and cellular localization of the Rhizobium host specificity-determining NodE protein. EMBO J. 1989 Oct;8(10):2811–2818. doi: 10.1002/j.1460-2075.1989.tb08427.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Staskawicz B. J., Dahlbeck D., Keen N. T. Cloned avirulence gene of Pseudomonas syringae pv. glycinea determines race-specific incompatibility on Glycine max (L.) Merr. Proc Natl Acad Sci U S A. 1984 Oct;81(19):6024–6028. doi: 10.1073/pnas.81.19.6024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tamaki S., Dahlbeck D., Staskawicz B., Keen N. T. Characterization and expression of two avirulence genes cloned from Pseudomonas syringae pv. glycinea. J Bacteriol. 1988 Oct;170(10):4846–4854. doi: 10.1128/jb.170.10.4846-4854.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vieira J., Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 1987;153:3–11. doi: 10.1016/0076-6879(87)53044-0. [DOI] [PubMed] [Google Scholar]

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