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. 1981 Sep;74(1):23–28. doi: 10.1111/j.1476-5381.1981.tb09951.x

Excitatory effect of a new polypeptide (anthopleurin-B) from sea anemone on the guinea-pig vas deferens.

T R Norton, Y Ohizumi, S Shibata
PMCID: PMC2071889  PMID: 7272600

Abstract

1 Anthopleurin-B (AP-B, greater than 3 x 10(-9) M), a newly isolated polypeptide from sea anemone (Anthopleura xanthogrammica), caused powerful rhythmic contractions in the guinea-pig isolated vas deferens. The other polypeptides anthopleurin-A from A. xanthogrammica and anthopleurin-C from A. elegantissima, elicited similar effects but in higher concentrations ( less than 5 x 10(-8) M). Toxin II (10(-6) M) isolated from the sea anemone, Anemonia sulcata, had no effect. 2 The rhythmic contractions induced by AP-B were inhibited by phentolamine, bretylium, guanethidine, reserpine, 6-hydroxydopamine, tetrodotoxin (TTX) and verapamil. Mecamylamine, atropine, methysergide, chlorpheniramine, and indomethacin had no effect. 3 AP-B (10(-8) M approximately 10(-5) M) caused a dose-dependent increase in the amount of endogenous noradrenaline (NA) released from the vas deferens. AP-B (10-5M) increased the amount of NA released to approximately 310 times (12 micrograms/g tissue) that of untreated tissues. 4 The AP-B-induced release of NA was inhibited or abolished by TTX, verapamil, or incubation in Ca-free medium. 5 These results suggest that the AP-B-induced rhythmic contraction of the vas deferens is mediated through the release of NA from adrenergic nerve endings; AP-B is one of the most potent substances in stimulating NA release from the vas deferens.

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Selected References

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  1. Ambache N., Zar M. A. Evidence against adrenergic motor transmission in the guinea-pig vas deferens. J Physiol. 1971 Jul;216(2):359–389. doi: 10.1113/jphysiol.1971.sp009530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BHARGAVA K. P., KAR K., PARMAR S. S. INDEPENDENT CHOLINERGIC AND ADRENERGIC MECHANISMS IN THE GUINEA-PIG ISOLATED NERVE-VAS DEFERENS PREPARATION. Br J Pharmacol Chemother. 1965 Jun;24:641–650. doi: 10.1111/j.1476-5381.1965.tb01620.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BIRMINGHAM A. T., WILSON A. B. PREGANGLIONIC AND POSTGANGLIONIC STIMULATION OF THE GUINEA-PIG ISOLATED VAS DEFERENS PREPARATION. Br J Pharmacol Chemother. 1963 Dec;21:569–580. doi: 10.1111/j.1476-5381.1963.tb02024.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bergman C., Dubois J. M., Rojas E., Rathmayer W. Decreased rate of sodium conductance inactivation in the node of Ranvier induced by a polypeptide toxin from sea anemone. Biochim Biophys Acta. 1976 Nov 11;455(1):173–184. doi: 10.1016/0005-2736(76)90162-0. [DOI] [PubMed] [Google Scholar]
  5. Blair R. W., Peterson D. F., Bishop V. S. The effects of anthopleurin-A on cardiac dynamics in conscious dogs. J Pharmacol Exp Ther. 1978 Nov;207(2):271–276. [PubMed] [Google Scholar]
  6. Béress L., Béress R., Wunderer G. Isolation and characterisation of three polypeptides with neurotoxic activity from Anemonia sulcata. FEBS Lett. 1975 Feb 15;50(3):311–314. doi: 10.1016/0014-5793(75)80517-5. [DOI] [PubMed] [Google Scholar]
  7. Endo Y., Ogura Y. Separation of biogenic amines in rat brain on a phosphorylated-cellulose column. Eur J Pharmacol. 1973 Mar;21(3):293–298. doi: 10.1016/0014-2999(73)90129-5. [DOI] [PubMed] [Google Scholar]
  8. HAEGGENDAL J. AN IMPROVED METHOD FOR FLUORIMETRIC DETERMINATION OF SMALL AMOUNTS OF ADRENALINE AND NORADRENALINE IN PLASMA AND TISSUES. Acta Physiol Scand. 1963 Nov;59:242–254. doi: 10.1111/j.1748-1716.1963.tb02739.x. [DOI] [PubMed] [Google Scholar]
  9. Haeusler G. Differential effect of verapamil on excitation-contraction coupling in smooth muscle and on excitation-secretion coupling in adrenergic nerve terminals. J Pharmacol Exp Ther. 1972 Mar;180(3):672–682. [PubMed] [Google Scholar]
  10. Iversen L. L., Langer S. Z. Effects of phenoxybenzamine on the uptake and metabolism of noradrenaline in the rat heart and vas deferens. Br J Pharmacol. 1969 Nov;37(3):627–637. doi: 10.1111/j.1476-5381.1969.tb08501.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jenkins D. A., Marshall I., Nasmyth P. A. An inhibitory role for noradrenaline in the mouse vas deferens. Br J Pharmacol. 1977 Dec;61(4):649–655. doi: 10.1111/j.1476-5381.1977.tb07558.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jones M. E., Spriggs T. L. Noradrenaline and motor transmission in the vas deferens of the mouse. Br J Pharmacol. 1975 Mar;53(3):323–331. [PMC free article] [PubMed] [Google Scholar]
  13. Kudo Y., Shibata S. The potent excitatory effect of a novel polypeptide, anthopleurin-B, isolated from a sea anemone (Anthopleura xanthogrammica) on the frog spinal cord. J Pharmacol Exp Ther. 1980 Aug;214(2):443–448. [PubMed] [Google Scholar]
  14. Low P. A., Wu C. H., Narahashi T. The effect of anthopleurin-A on crayfish giant axon. J Pharmacol Exp Ther. 1979 Sep;210(3):417–421. [PubMed] [Google Scholar]
  15. McGrath J. C. Adrenergic and 'non-adrenergic' components in the contractile response of the vas deferens to a single indirect stimulus. J Physiol. 1978 Oct;283:23–39. doi: 10.1113/jphysiol.1978.sp012486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nachshen D. A., Blaustein M. P. The effects of some organic "calcium antagonists" on calcium influx in presynaptic nerve terminals. Mol Pharmacol. 1979 Sep;16(2):576–586. [PubMed] [Google Scholar]
  17. Norton T. R., Shibata S., Kashiwagi M., Bentley J. Isolation and characterization of the cardiotonic polypeptide anthopleurin-A from the sea anemone Anthopleura xanthogrammica. J Pharm Sci. 1976 Sep;65(9):1368–1374. doi: 10.1002/jps.2600650927. [DOI] [PubMed] [Google Scholar]
  18. Scriabine A., Van Arman C. G., Morgan G., Morris A. A., Bennett C. D., Bohidar N. R. Cardiotonic effects of anthopleurin-A, a polypeptide from a sea anemone. J Cardiovasc Pharmacol. 1979 Sep-Oct;1(5):571–583. doi: 10.1097/00005344-197909000-00009. [DOI] [PubMed] [Google Scholar]
  19. Shibata S., Dunn D. F., Kuchii M., Kashiwagi M., Norton T. R. Cardiac stimulant action of extracts of coelenterates on rat atria. J Pharm Sci. 1974 Aug;63(8):1332–1333. doi: 10.1002/jps.2600630845. [DOI] [PubMed] [Google Scholar]
  20. Shibata S., Izumi T., Seriguchi D. G., Norton T. R. Further studies on the positive inotropic effect of the polypeptide anthopleurin-A from a sea anemone. J Pharmacol Exp Ther. 1978 Jun;205(3):683–692. [PubMed] [Google Scholar]
  21. Shibata S., Norton T. R., Izumi T., Matsuo T., Katsuki S. A polypeptide (AP-A) from sea anemone (Anthopleura xanthogrammica) with potent positive inotropic action. J Pharmacol Exp Ther. 1976 Nov;199(2):298–309. [PubMed] [Google Scholar]
  22. Shimizu T., Iwamura N., Toyama J., Yamada K., Shibata S. Effect of cardiotonic polypeptide anthopleurin-A on canine Purkinje and ventricular muscle fibers. Eur J Pharmacol. 1979 Jun;56(1-2):7–13. doi: 10.1016/0014-2999(79)90426-6. [DOI] [PubMed] [Google Scholar]
  23. Tanaka M., Hainu M., Yasunobu K. T., Norton T. R. Amino acid sequence of the Anthopleura xanthogrammica heart stimulant, anthopleurin A. Biochemistry. 1977 Jan 25;16(2):204–208. doi: 10.1021/bi00621a007. [DOI] [PubMed] [Google Scholar]
  24. Van Der Kloot W., Kita H. The effects of the "calcium-antagonist" verapamil on muscle action potentials in the frog and crayfish and on neuromuscular transmission in the crayfish. Comp Biochem Physiol C. 1975 Jan 1;50(1):121–125. [PubMed] [Google Scholar]
  25. Wakade A. R., Krusz J. Effect of reserpine, phenoxybenzamine and cocaine on neuromuscular transmission in the vas deferens of the guinea pig. J Pharmacol Exp Ther. 1972 May;181(2):310–317. [PubMed] [Google Scholar]
  26. Wunderer G., Machleidt W., Wachter E. Toxin II from Anemonia sulcata-the first sequence of a coelenterate toxin. Hoppe Seylers Z Physiol Chem. 1976 Feb;357(2):239–240. [PubMed] [Google Scholar]
  27. von Euler U. S., Hedqvist P. Evidence for an alpha- and beta2-receptor mediated inhibition of the twitch response in the guinea pig vas deferens by noradrenaline. Acta Physiol Scand. 1975 Apr;93(4):572–573. doi: 10.1111/j.1748-1716.1975.tb05853.x. [DOI] [PubMed] [Google Scholar]

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