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. 1991 Jan;173(1):391–393. doi: 10.1128/jb.173.1.391-393.1991

A newly identified DNA replication terminus site, TerE, on the Escherichia coli chromosome.

M Hidaka 1, T Kobayashi 1, T Horiuchi 1
PMCID: PMC207198  PMID: 1824765

Abstract

To search for heretofore unidentified DNA replication termination (Ter) sites on the Escherichia coli chromosome, we screened the entire Kohara lambda bacteriophage library using as probes the four known 22-bp Ter sequences. We found a Ter site, which we named TerE, located at 23.2 min on the linkage map. TerE inhibits only counterclockwise DNA replication. Macroscopically, five Ter sites are located in a periodic arrangement on the genome.

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Selected References

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  1. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 8. Microbiol Rev. 1990 Jun;54(2):130–197. doi: 10.1128/mr.54.2.130-197.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bastia D., Germino J., Crosa J. H., Ram J. The nucleotide sequence surrounding the replication terminus of R6K. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2095–2099. doi: 10.1073/pnas.78.4.2095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brewer B. J., Fangman W. L. A replication fork barrier at the 3' end of yeast ribosomal RNA genes. Cell. 1988 Nov 18;55(4):637–643. doi: 10.1016/0092-8674(88)90222-x. [DOI] [PubMed] [Google Scholar]
  4. Carrigan C. M., Haarsma J. A., Smith M. T., Wake R. G. Sequence features of the replication terminus of the Bacillus subtilis chromosome. Nucleic Acids Res. 1987 Oct 26;15(20):8501–8509. doi: 10.1093/nar/15.20.8501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. François V., Louarn J., Louarn J. M. The terminus of the Escherichia coli chromosome is flanked by several polar replication pause sites. Mol Microbiol. 1989 Aug;3(8):995–1002. doi: 10.1111/j.1365-2958.1989.tb00250.x. [DOI] [PubMed] [Google Scholar]
  6. Germino J., Bastia D. Termination of DNA replication in vitro at a sequence-specific replication terminus. Cell. 1981 Mar;23(3):681–687. doi: 10.1016/0092-8674(81)90431-1. [DOI] [PubMed] [Google Scholar]
  7. Hernández P., Lamm S. S., Bjerknes C. A., Hof J. V. Replication termini in the rDNA of synchronized pea root cells (Pisum sativum). EMBO J. 1988 Feb;7(2):303–308. doi: 10.1002/j.1460-2075.1988.tb02813.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hidaka M., Akiyama M., Horiuchi T. A consensus sequence of three DNA replication terminus sites on the E. coli chromosome is highly homologous to the terR sites of the R6K plasmid. Cell. 1988 Nov 4;55(3):467–475. doi: 10.1016/0092-8674(88)90033-5. [DOI] [PubMed] [Google Scholar]
  9. Hidaka M., Kobayashi T., Takenaka S., Takeya H., Horiuchi T. Purification of a DNA replication terminus (ter) site-binding protein in Escherichia coli and identification of the structural gene. J Biol Chem. 1989 Dec 15;264(35):21031–21037. [PubMed] [Google Scholar]
  10. Hill T. M., Henson J. M., Kuempel P. L. The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication. Proc Natl Acad Sci U S A. 1987 Apr;84(7):1754–1758. doi: 10.1073/pnas.84.7.1754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hill T. M., Pelletier A. J., Tecklenburg M. L., Kuempel P. L. Identification of the DNA sequence from the E. coli terminus region that halts replication forks. Cell. 1988 Nov 4;55(3):459–466. doi: 10.1016/0092-8674(88)90032-3. [DOI] [PubMed] [Google Scholar]
  12. Hill T. M., Tecklenburg M. L., Pelletier A. J., Kuempel P. L. tus, the trans-acting gene required for termination of DNA replication in Escherichia coli, encodes a DNA-binding protein. Proc Natl Acad Sci U S A. 1989 Mar;86(5):1593–1597. doi: 10.1073/pnas.86.5.1593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Horiuchi T., Hidaka M. Core sequence of two separable terminus sites of the R6K plasmid that exhibit polar inhibition of replication is a 20 bp inverted repeat. Cell. 1988 Aug 12;54(4):515–523. doi: 10.1016/0092-8674(88)90073-6. [DOI] [PubMed] [Google Scholar]
  14. Khatri G. S., MacAllister T., Sista P. R., Bastia D. The replication terminator protein of E. coli is a DNA sequence-specific contra-helicase. Cell. 1989 Nov 17;59(4):667–674. doi: 10.1016/0092-8674(89)90012-3. [DOI] [PubMed] [Google Scholar]
  15. Knott V., Blake D. J., Brownlee G. G. Completion of the detailed restriction map of the E. coli genome by the isolation of overlapping cosmid clones. Nucleic Acids Res. 1989 Aug 11;17(15):5901–5912. doi: 10.1093/nar/17.15.5901. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kobayashi T., Hidaka M., Horiuchi T. Evidence of a ter specific binding protein essential for the termination reaction of DNA replication in Escherichia coli. EMBO J. 1989 Aug;8(8):2435–2441. doi: 10.1002/j.1460-2075.1989.tb08374.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kohara Y., Akiyama K., Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987 Jul 31;50(3):495–508. doi: 10.1016/0092-8674(87)90503-4. [DOI] [PubMed] [Google Scholar]
  18. Lee E. H., Kornberg A., Hidaka M., Kobayashi T., Horiuchi T. Escherichia coli replication termination protein impedes the action of helicases. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9104–9108. doi: 10.1073/pnas.86.23.9104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lewis P. J., Smith M. T., Wake R. G. A protein involved in termination of chromosome replication in Bacillus subtilis binds specifically to the terC site. J Bacteriol. 1989 Jun;171(6):3564–3567. doi: 10.1128/jb.171.6.3564-3567.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sista P. R., Mukherjee S., Patel P., Khatri G. S., Bastia D. A host-encoded DNA-binding protein promotes termination of plasmid replication at a sequence-specific replication terminus. Proc Natl Acad Sci U S A. 1989 May;86(9):3026–3030. doi: 10.1073/pnas.86.9.3026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Weiss A. S., Wake R. G. A unique DNA intermediate associated with termination of chromosome replication in Bacillus subtilis. Cell. 1984 Dec;39(3 Pt 2):683–689. doi: 10.1016/0092-8674(84)90475-6. [DOI] [PubMed] [Google Scholar]
  22. de Massy B., Béjar S., Louarn J., Louarn J. M., Bouché J. P. Inhibition of replication forks exiting the terminus region of the Escherichia coli chromosome occurs at two loci separated by 5 min. Proc Natl Acad Sci U S A. 1987 Apr;84(7):1759–1763. doi: 10.1073/pnas.84.7.1759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. de Massy B., Fayet O., Kogoma T. Multiple origin usage for DNA replication in sdrA(rnh) mutants of Escherichia coli K-12. Initiation in the absence of oriC. J Mol Biol. 1984 Sep 15;178(2):227–236. doi: 10.1016/0022-2836(84)90141-4. [DOI] [PubMed] [Google Scholar]

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