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. 1991 Jan;173(1):412–416. doi: 10.1128/jb.173.1.412-416.1991

Temperature sensitivity caused by missense suppressor supH and amber suppressor supP in Escherichia coli.

S Thorbjarnardóttir 1, A Björnsson 1, L Amundadóttir 1, G Eggertsson 1
PMCID: PMC207204  PMID: 1987132

Abstract

The temperature-sensitive missense suppressor supH and amber suppressor supP in Escherichia coli are mutations of the serU and leuX genes, respectively. The supH tRNA, tRNA(SerCAA), is expected to recognize UUG codons, which are normally read by tRNA(LeuCAA) and tRNA(LeuUAA), coded for by the leuX gene and the leuZ gene, respectively. We show that supP and supH are incompatible and that strains carrying both supP and a restrictive rpsL allele are temperature sensitive. It is suggested that the temperature sensitivity of both supH and supP strains is caused by deficient reading of UUG codons by tRNA(LeuUAA).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aota S., Gojobori T., Ishibashi F., Maruyama T., Ikemura T. Codon usage tabulated from the GenBank Genetic Sequence Data. Nucleic Acids Res. 1988;16 (Suppl):r315–r402. doi: 10.1093/nar/16.suppl.r315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 8. Microbiol Rev. 1990 Jun;54(2):130–197. doi: 10.1128/mr.54.2.130-197.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Biswas D. K., Gorini L. Restriction, de-restriction and mistranslation in missense suppression. Ribosomal discrimination of transfer RNA's. J Mol Biol. 1972 Feb 28;64(1):119–134. doi: 10.1016/0022-2836(72)90324-5. [DOI] [PubMed] [Google Scholar]
  4. Blank H. U., Söll D. Purification of five leucine transfer ribonucleic acid species from Escherichia coli and their acylation by heterologous leucyl-transfer ribonucleic acid synthetase. J Biol Chem. 1971 Aug 25;246(16):4947–4950. [PubMed] [Google Scholar]
  5. Crick F. H. Codon--anticodon pairing: the wobble hypothesis. J Mol Biol. 1966 Aug;19(2):548–555. doi: 10.1016/s0022-2836(66)80022-0. [DOI] [PubMed] [Google Scholar]
  6. Drapeau G. R., Chausseau J. P., Gariépy F. Unusual properties of a new division mutant of Escherichia coli. Can J Microbiol. 1983 Jun;29(6):694–699. doi: 10.1139/m83-113. [DOI] [PubMed] [Google Scholar]
  7. Eggertsson G. Suppressors causing temperature sensitivity of growth in Escherichia coli. Genetics. 1968 Oct;60(2):269–280. doi: 10.1093/genetics/60.2.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eggertsson G., Söll D. Transfer ribonucleic acid-mediated suppression of termination codons in Escherichia coli. Microbiol Rev. 1988 Sep;52(3):354–374. doi: 10.1128/mr.52.3.354-374.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goldman E., Holmes W. M., Hatfield G. W. Specificity of codon recognition by Escherichia coli tRNALeu isoaccepting species determined by protein synthesis in vitro directed by phage RNA. J Mol Biol. 1979 Apr 25;129(4):567–585. doi: 10.1016/0022-2836(79)90469-8. [DOI] [PubMed] [Google Scholar]
  10. Gopinathan K. P., Garen A. A leucyl-transfer RNA specified by the amber suppressor gene Su6+. J Mol Biol. 1970 Feb 14;47(3):393–401. doi: 10.1016/0022-2836(70)90310-4. [DOI] [PubMed] [Google Scholar]
  11. Gorini L. Informational suppression. Annu Rev Genet. 1970;4:107–134. doi: 10.1146/annurev.ge.04.120170.000543. [DOI] [PubMed] [Google Scholar]
  12. Kleina L. G., Masson J. M., Normanly J., Abelson J., Miller J. H. Construction of Escherichia coli amber suppressor tRNA genes. II. Synthesis of additional tRNA genes and improvement of suppressor efficiency. J Mol Biol. 1990 Jun 20;213(4):705–717. doi: 10.1016/S0022-2836(05)80257-8. [DOI] [PubMed] [Google Scholar]
  13. Komine Y., Adachi T., Inokuchi H., Ozeki H. Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12. J Mol Biol. 1990 Apr 20;212(4):579–598. doi: 10.1016/0022-2836(90)90224-A. [DOI] [PubMed] [Google Scholar]
  14. Leclerc G., Sirard C., Drapeau G. R. The Escherichia coli cell division mutation ftsM1 is in serU. J Bacteriol. 1989 Apr;171(4):2090–2095. doi: 10.1128/jb.171.4.2090-2095.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Murray N. E., Brammar W. J., Murray K. Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet. 1977 Jan 7;150(1):53–61. doi: 10.1007/BF02425325. [DOI] [PubMed] [Google Scholar]
  16. Normanly J., Masson J. M., Kleina L. G., Abelson J., Miller J. H. Construction of two Escherichia coli amber suppressor genes: tRNAPheCUA and tRNACysCUA. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6548–6552. doi: 10.1073/pnas.83.17.6548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Petrullo L. A., Gallagher P. J., Elseviers D. The role of 2-methylthio-N6-isopentenyladenosine in readthrough and suppression of nonsense codons in Escherichia coli. Mol Gen Genet. 1983;190(2):289–294. doi: 10.1007/BF00330653. [DOI] [PubMed] [Google Scholar]
  18. Rosset R., Gorini L. A ribosomal ambiguity mutation. J Mol Biol. 1969 Jan 14;39(1):95–112. doi: 10.1016/0022-2836(69)90336-2. [DOI] [PubMed] [Google Scholar]
  19. Steege D. A. A nucleotide change in the anticodon of an Escherichia coli serine transfer RNA results in supD-amber suppression. Nucleic Acids Res. 1983 Jun 11;11(11):3823–3832. doi: 10.1093/nar/11.11.3823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Strigini P., Gorini L. Ribosomal mutations affecting efficiency of amber suppression. J Mol Biol. 1970 Feb 14;47(3):517–530. doi: 10.1016/0022-2836(70)90319-0. [DOI] [PubMed] [Google Scholar]
  21. Thorbjarnardóttir S., Dingermann T., Rafnar T., Andrésson O. S., Söll D., Eggertsson G. Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene. J Bacteriol. 1985 Jan;161(1):219–222. doi: 10.1128/jb.161.1.219-222.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Thorbjarnardóttir S., Uemura H., Dingermann T., Rafnar T., Thorsteinsdóttir S., Söll D., Eggertsson G. Escherichia coli supH suppressor: temperature-sensitive missense suppression caused by an anticodon change in tRNASer2. J Bacteriol. 1985 Jan;161(1):207–211. doi: 10.1128/jb.161.1.207-211.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yoshimura M., Inokuchi H., Ozeki H. Identification of transfer RNA suppressors in Escherichia coli. IV. Amber suppressor Su+6 a double mutant of a new species of leucine tRNA. J Mol Biol. 1984 Aug 25;177(4):627–644. doi: 10.1016/0022-2836(84)90041-x. [DOI] [PubMed] [Google Scholar]

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