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. 1969 Oct;50(5):507–515.

An Oestrogen-linked Nephrocalcinosis in Rats

C P Geary, F B Cousins
PMCID: PMC2072145  PMID: 5348008

Abstract

A non-familial nephrocalcinosis, unrelated to hypercalcaemia, and occurring in female rats only, is described. Oestrogens were found to be essential since ovariectomised rats did not develop the condition. Replacement therapy with oestrogens following gonadectomy in animals of either sex resulted in kidney calcification. There is also evidence that an unidentified dietary factor is concerned in the initiation of the nephrocalcinosis. Histological appearance of mineral deposits coincided with increments in renal calcium and phosphorus. Grossly, calcification commenced at the cortico-medullary junction, spreading to involve the medulla. Electron microscopy revealed the presence of both intratubular and intracellular lamellated deposits in the nephron. These deposits were isolated by density centrifugation and chemically characterised.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEKEMEIER H., LEISER H. [Local changes in experimental calcium deposits in the kidneys under the influence of estrogens]. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1961;241:146–148. [PubMed] [Google Scholar]
  2. BOAS N. F. Method for the determination of hexosamines in tissues. J Biol Chem. 1953 Oct;204(2):553–563. [PubMed] [Google Scholar]
  3. BOYCE W. H., GARVEY F. K. The amount and nature of the organic matrix in urinary calculi: a review. J Urol. 1956 Sep;76(3):213–227. doi: 10.1016/S0022-5347(17)66686-2. [DOI] [PubMed] [Google Scholar]
  4. Bekemeier H. Nephrocalcitopotrope Wirkung von Athinylnortestosteron und Progesteron. Naturwissenschaften. 1965 Jul;52(13):397–397. [PubMed] [Google Scholar]
  5. Cousins F. B., Geary C. P. A sex-determined renal calcification in rats. Nature. 1966 Aug 27;211(5052):980–981. doi: 10.1038/211980b0. [DOI] [PubMed] [Google Scholar]
  6. GROB H. S., KUPPERMANN H. S., GORDON A. S. CALCIFICATION OF THE OVARIAN FOLLICLE IN RATS. Proc Soc Exp Biol Med. 1964 Jul;116:766–768. doi: 10.3181/00379727-116-29368. [DOI] [PubMed] [Google Scholar]
  7. HEPPEL L. A., HILMOE R. J. Purification of yeast inorganic pyrophosphatase. J Biol Chem. 1951 Sep;192(1):87–94. [PubMed] [Google Scholar]
  8. Kraus P. A simple modified micromethod for the determination of citrate. Clin Chim Acta. 1965 Oct;12(4):462–465. doi: 10.1016/0009-8981(65)90137-3. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. LUDWIG T. G., MALTHUS R. S., HEALY W. B. An association between soil conditions and dental caries in rats. Nature. 1962 May 5;194:456–458. doi: 10.1038/194456a0. [DOI] [PubMed] [Google Scholar]
  11. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lifshitz F., Harrison H. C., Bull E. C., Harrison H. E. Citrate metabolism and the mechanism of renal calcification induced by magnesium depletion. Metabolism. 1967 Apr;16(4):345–357. doi: 10.1016/0026-0495(67)90046-7. [DOI] [PubMed] [Google Scholar]
  13. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SCHNEEBERGER E. E., MORRISON A. B. THE NEPHROPATHY OF EXPERIMENTAL MAGNESIUM DEFICIENCY: LIGHT AND ELECTRON MICROSCOPIC INVESTIGATIONS. Lab Invest. 1965 Jun;14:674–686. [PubMed] [Google Scholar]
  15. STERNBERG W. H., FARBER E., DUNLAP C. E. Histochemical localization of specific oxidative enzymes. II. Localization of diphosphopyridine nucleotide and triphosphopyridine nucleotide diaphorases and the succindehydrogenase system in the kidney. J Histochem Cytochem. 1956 May;4(3):266–283. doi: 10.1177/4.3.266. [DOI] [PubMed] [Google Scholar]
  16. WELT L. G. EXPERIMENTAL MAGNESIUM DEPLETION. Yale J Biol Med. 1964 Apr;36:325–349. [PMC free article] [PubMed] [Google Scholar]
  17. WHANG R., WELT L. G. Observations in experimental magnesium depletion. J Clin Invest. 1963 Mar;42:305–313. doi: 10.1172/JCI104717. [DOI] [PMC free article] [PubMed] [Google Scholar]

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