Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1991 Feb;173(3):1021–1026. doi: 10.1128/jb.173.3.1021-1026.1991

The Escherichia coli mraY gene encoding UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase.

M Ikeda 1, M Wachi 1, H K Jung 1, F Ishino 1, M Matsuhashi 1
PMCID: PMC207220  PMID: 1846850

Abstract

Amplification of the mraY gene, previously called open reading frame Y (ORF-Y, 1,080 bp), at 2 min in the chromosome map of Escherichia coli enhanced the activity of UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase (EC 2.7.8.13). This enzyme catalyzes the formation of undecaprenyl-pyrophosphoryl-N-acetylmuramoyl-pentapeptide from UDP-N-acetylmuramoyl-pentapeptide and undecaprenyl-phosphate, the first step in the lipid cycle reactions in biosynthesis of bacterial cell wall peptidoglycans. The enhanced enzyme activity was sensitive to tunicamycin, and the amino tunicamycin-sensitive N-acetylglucosamine-1-phosphate transferase of Saccharomyces cerevisiae. Very probably mraY is the structural gene for the above enzyme.

Full text

PDF
1021

Images in this article

1022Image
on p.1022

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON J. S., MATSUHASHI M., HASKIN M. A., STROMINGER J. L. LIPID-PHOSPHOACETYLMURAMYL-PENTAPEPTIDE AND LIPID-PHOSPHODISACCHARIDE-PENTAPEPTIDE: PRESUMED MEMBRANE TRANSPORT INTERMEDIATES IN CELL WALL SYNTHESIS. Proc Natl Acad Sci U S A. 1965 Apr;53:881–889. doi: 10.1073/pnas.53.4.881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DeVries J. K., Zubay G. DNA-directed peptide synthesis. II. The synthesis of the alpha-fragment of the enzyme beta-galactosidase. Proc Natl Acad Sci U S A. 1967 Apr;57(4):1010–1012. doi: 10.1073/pnas.57.4.1010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hartog K. O., Bishop B. Genomic sequence coding for tunicamycin resistance in yeast. Nucleic Acids Res. 1987 Apr 24;15(8):3627–3627. doi: 10.1093/nar/15.8.3627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ikeda M., Sato T., Wachi M., Jung H. K., Ishino F., Kobayashi Y., Matsuhashi M. Structural similarity among Escherichia coli FtsW and RodA proteins and Bacillus subtilis SpoVE protein, which function in cell division, cell elongation, and spore formation, respectively. J Bacteriol. 1989 Nov;171(11):6375–6378. doi: 10.1128/jb.171.11.6375-6378.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ikeda M., Wachi M., Ishino F., Matsuhashi M. Nucleotide sequence involving murD and an open reading frame ORF-Y spacing murF and ftsW in Escherichia coli. Nucleic Acids Res. 1990 Feb 25;18(4):1058–1058. doi: 10.1093/nar/18.4.1058. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ikeda M., Wachi M., Jung H. K., Ishino F., Matsuhashi M. Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli. Nucleic Acids Res. 1990 Jul 11;18(13):4014–4014. doi: 10.1093/nar/18.13.4014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ishino F., Jung H. K., Ikeda M., Doi M., Wachi M., Matsuhashi M. New mutations fts-36, lts-33, and ftsW clustered in the mra region of the Escherichia coli chromosome induce thermosensitive cell growth and division. J Bacteriol. 1989 Oct;171(10):5523–5530. doi: 10.1128/jb.171.10.5523-5530.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  9. Lipman D. J., Pearson W. R. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. doi: 10.1126/science.2983426. [DOI] [PubMed] [Google Scholar]
  10. Matsuhashi M., Wachi M., Ishino F. Machinery for cell growth and division: penicillin-binding proteins and other proteins. Res Microbiol. 1990 Jan;141(1):89–103. doi: 10.1016/0923-2508(90)90101-u. [DOI] [PubMed] [Google Scholar]
  11. Mengin-Lecreulx D., Texier L., van Heijenoort J. Nucleotide sequence of the cell-envelope murG gene of Escherichia coli. Nucleic Acids Res. 1990 May 11;18(9):2810–2810. doi: 10.1093/nar/18.9.2810. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mengin-Lecreulx D., van Heijenoort J. Nucleotide sequence of the murD gene encoding the UDP-MurNAc-L-Ala-D-Glu synthetase of Escherichia coli. Nucleic Acids Res. 1990 Jan 11;18(1):183–183. doi: 10.1093/nar/18.1.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Miyakawa T., Matsuzawa H., Matsuhashi M., Sugino Y. Cell wall peptidoglycan mutants of Escherichia coli K-12: existence of two clusters of genes, mra and mrb, for cell wall peptidoglycan biosynthesis. J Bacteriol. 1972 Nov;112(2):950–958. doi: 10.1128/jb.112.2.950-958.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nakamura M., Maruyama I. N., Soma M., Kato J., Suzuki H., Horota Y. On the process of cellular division in Escherichia coli: nucleotide sequence of the gene for penicillin-binding protein 3. Mol Gen Genet. 1983;191(1):1–9. doi: 10.1007/BF00330881. [DOI] [PubMed] [Google Scholar]
  15. Parquet C., Flouret B., Mengin-Lecreulx D., van Heijenoort J. Nucleotide sequence of the murF gene encoding the UDP-MurNAc-pentapeptide synthetase of Escherichia coli. Nucleic Acids Res. 1989 Jul 11;17(13):5379–5379. doi: 10.1093/nar/17.13.5379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sancar A., Hack A. M., Rupp W. D. Simple method for identification of plasmid-coded proteins. J Bacteriol. 1979 Jan;137(1):692–693. doi: 10.1128/jb.137.1.692-693.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tao J. S., Ishiguro E. E. Nucleotide sequence of the murE gene of Escherichia coli. Can J Microbiol. 1989 Nov;35(11):1051–1054. doi: 10.1139/m89-175. [DOI] [PubMed] [Google Scholar]
  18. Vieira J., Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 1987;153:3–11. doi: 10.1016/0076-6879(87)53044-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES