Abstract
Organ cultures of mouse and human embryo alimentary tract have been prepared and the mucosal epithelium shown to survive in a modified form for up to 12 days.
Cultures of mouse tissue supported the growth of EMC and Sendai viruses, and those of human tissue the growth of poliovirus 1, coxsackievirus B3, echovirus 11, Sendai virus and adenovirus 5. Good growth was obtained in human tissue inoculated up to 4 days after preparation. Growth in roller tube cultures was similar to that in cultures set up in Petri dishes. Addition of folic acid and vitamin A had no effect on growth.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- FELL H. B., MELLANBY E. Metaplasia produced in cultures of chick ectoderm by high vitamin A. J Physiol. 1953 Mar;119(4):470–488. doi: 10.1113/jphysiol.1953.sp004860. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fogel A., Plotkin S. A. Genetic changes in attenuated poliovirus strains cultivated on human intestine in vitro. Am J Epidemiol. 1968 Mar;87(2):385–395. doi: 10.1093/oxfordjournals.aje.a120829. [DOI] [PubMed] [Google Scholar]
- HUANG J. S., BANG F. B. THE SUSCEPTIBILITY OF CHICK EMBRYO SKIN ORGAN CULTURES TO INFLUENZA VIRUS FOLLOWING EXCESS VITAMIN A. J Exp Med. 1964 Aug 1;120:129–148. doi: 10.1084/jem.120.2.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoorn B., Tyrrell D. A. Organ cultures in virology. Prog Med Virol. 1969;11:408–450. [PubMed] [Google Scholar]
- MARTIN E. M., MALEC J., SVED S., WORK T. S. Studies on protein and nucleic acid metabolism in virus-infected mammalian cells. 1. Encephalomyocarditis virus in Krebs II mouse-ascites-tumour cells. Biochem J. 1961 Sep;80:585–597. doi: 10.1042/bj0800585. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tyrrell D. A., Blamire C. J. Improvements in a method of growing respiratory viruses in organ cultures. Br J Exp Pathol. 1967 Apr;48(2):217–227. [PMC free article] [PubMed] [Google Scholar]