Abstract
The cytotoxicity of aflatoxins G2 and B2 to cultured human embryo liver cells and of aflatoxin B1 to cultured adult liver cells, to cells of a semicontinuous diploid liver cell line and to a diploid lung cell line have been determined.
Aflatoxins B2 and G2 were found to be considerably less toxic to human embryo liver cells than aflatoxin B1 and the LD50's were 35 and 10 μg./ml. respectively after 48 hr exposure to the toxin. Human adult liver cells in culture, cells of the liver cell line and lung cells were much less sensitive to the action of aflatoxin B1 than embryo liver cells.
These results have been compared with those previously reported for B1 and G1 and the reasons for the variation in toxicity observed are discussed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Clifford J. I., Rees K. R., Stevens M. E. The effect of the aflatoxins B1, G1, and G2 on protein and nucleic acid synthesis in rat liver. Biochem J. 1967 Apr;103(1):258–261. doi: 10.1042/bj1030258. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McLean A. E., McLean E. K. The effect of diet and 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) on microsomal hydroxylating enzymes and on sensitivity of rats to carbon tetrachloride poisoning. Biochem J. 1966 Aug;100(2):564–571. doi: 10.1042/bj1000564. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sullman S. F., Zuckerman A. J., Taylor P. F., Stubbs J. Tissue culture of adult liver biopsies. J Clin Pathol. 1968 Nov;21(6):794–794. doi: 10.1136/jcp.21.6.794-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sullman S. F., Zuckerman A. J. The effect of heliotrine, a pyrrolizidine alkaloid, on human liver cells in culture. Br J Exp Pathol. 1969 Aug;50(4):361–370. [PMC free article] [PubMed] [Google Scholar]
- Wogan G. N. Chemical nature and biological effects of the aflatoxins. Bacteriol Rev. 1966 Jun;30(2):460–470. doi: 10.1128/br.30.2.460-470.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]