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. 1971 Dec;52(6):684–695.

Dextran Sulphate Osteopathy in Parathyroidectomised Rats

H A Ellis, Kathleen M Peart
PMCID: PMC2072386  PMID: 5135539

Abstract

The synthetic heparin substitute dextran sulphate induces osteoporosis after prolonged administration to experimental animals. The possibility that this is brought about indirectly by hypocalcaemia and secondary hyperparathyroidism as a result of the binding of ionised calcium by dextran sulphate has been studied in rats.

Twenty-four rats were given 25 mg. per kg. per day of sodium dextran sulphate for periods up to 11 weeks. Sixteen were given the same amount of dextran sulphate but were previously parathyroidectomised. Two control groups comprised 17 intact and 15 parathyroidectomised rats, respectively. The limb bones were examined histologically and their sizes and growth rates measured from radiographs and by tetracycline labelling. The parathyroid glands from intact control and dextran sulphate treated rats were compared histologically and the serum calcium estimated in each animal.

All rats given dextran sulphate showed thinning of the metaphyseal cortical bone as a result of impaired endochondral ossification. Parathyroidectomised rats continued to grow and the lengths of their limb bones were unaffected. Parathyroidectomy had no influence on the occurrence or severity of bone changes attributed to dextran sulphate. Furthermore, serum calcium remained normal and there was no evidence of parathyroid hyperplasia in the intact dextran sulphate treated rats.

It is concluded that dextran sulphate induces osteoporosis in growing animals by interfering with endochondral ossification and does not induce hypocalcaemia and secondary hyperparathyroidism.

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688-1Figs. 2-4
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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADAMS S. S., THORPE H. M., GLYNN L. E. Effect of a laminarin sulfphate (LM 46) on bone growth. Lancet. 1958 Sep 20;2(7047):618–620. doi: 10.1016/s0140-6736(58)90339-8. [DOI] [PubMed] [Google Scholar]
  2. Ellis H. A., Lawrence J. C. Uptake in vitro of sulphur-35 (35-S) by the skin of guinea-pigs treated with dextran sulphate. J Pathol Bacteriol. 1966 Jul;92(1):217–220. doi: 10.1002/path.1700920123. [DOI] [PubMed] [Google Scholar]
  3. GILLMAN T., NAIDOO S. S. In vitro effects of heparin and calcium ions on lipaemic serum. Nature. 1957 May 4;179(4566):904–905. doi: 10.1038/179904a0. [DOI] [PubMed] [Google Scholar]
  4. GOLDHABER P. HEPARIN ENHANCEMENT OF FACTORS STIMULATING BONE RESORPTION IN TISSUE CULTURE. Science. 1965 Jan 22;147(3656):407–408. doi: 10.1126/science.147.3656.407. [DOI] [PubMed] [Google Scholar]
  5. HINT H. C., RICHTER A. W. Chronic toxicity of dextran sulphate in rabbits. Br J Pharmacol Chemother. 1958 Jun;13(2):109–112. doi: 10.1111/j.1476-5381.1958.tb00203.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JAFFE M. D., WILLIS P. W., 3rd MULTIPLE FRACTURES ASSOCIATED WITH LONG-TERM SODIUM HEPARIN THERAPY. JAMA. 1965 Jul 12;193:158–160. doi: 10.1001/jama.1965.03090020072024. [DOI] [PubMed] [Google Scholar]
  7. JOWSEY J., ROWLAND R. E., MARSHALL J. H., McLEAN F. C. The effect of parathyroidectomy on haversian remodeling of bone. Endocrinology. 1958 Dec;63(6):903–908. doi: 10.1210/endo-63-6-903. [DOI] [PubMed] [Google Scholar]
  8. Jha G. J., Deo M. G., Ramalingaswami V. Bone growth in protein deficiency. A study in rhesus monkeys. Am J Pathol. 1968 Dec;53(6):1111–1123. [PMC free article] [PubMed] [Google Scholar]
  9. KENNY A. D. Survival and serum calcium levels of rats after parathyroidectomy. Endocrinology. 1962 May;70:715–722. doi: 10.1210/endo-70-5-715. [DOI] [PubMed] [Google Scholar]
  10. Lindsay S., Nichols C. W. Medullary thyroid carcinoma and parathyroid hyperplasia in rats. Arch Pathol. 1969 Oct;88(4):402–406. [PubMed] [Google Scholar]
  11. Schuster J., Meier-Ruge W., Egli F. Zur Pathologie der Osteopathie nach Heparinbehandlung. Dtsch Med Wochenschr. 1969 Nov 7;94(45):2334–2338. doi: 10.1055/s-0028-1110442. [DOI] [PubMed] [Google Scholar]
  12. TOURTELLOTTE C. D., DZIEWIATKOWSKI D. D. A DISORDER OF ENDOCHONDRAL OSSIFICATION INDUCED BY DEXTRAN SULPHATE. J Bone Joint Surg Am. 1965 Sep;47:1185–1202. [PubMed] [Google Scholar]

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