Abstract
Limited treatment with papain has been used to liberate isolated epithelial cells from the epithelial mucosa of the rabbit small intestine. Such cell populations retain their brush borders, have a Qo2 of -10 μl O2/mg protein/hour and incorporate d[14C]glucosamine into three high molecular weight components having certain electrophoretic properties characteristic of glycoproteins. This system should be valuable for studying glycoprotein metabolism in epithelial cells without interference from sub-epithelial tissues.
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