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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1973 Aug;54(4):359–367.

The Effect of Scald Injury upon the Distribution of Glucose Between Red Cells and Plasma and Upon the Turnover of Glucose in Red Cells in the Rat

D F Heath
PMCID: PMC2072447  PMID: 4726090

Abstract

The acute effects of burn injury on the transport and turnover of glucose in red cells in vivo have been studied in the rat 1·5-5 hours after injury. Post-absorptive rats received a dorsal full thickness burn to 20% of the body surface, which reduced the red cell volume from 2·46 to 2·12 ml/100 g body weight and the plasma volume from 3·72 to 2·49 ml/100 g (without affecting Fcells) and increased the plasma glucose concentration (Cp) from 1·6 to 2·9 mg/ml.

In controls the glucose concentration in red cells (Cr) was 0·48 mg/ml, independent of the value of Cp. The Cr/Cp ratio was about 0·3, i.e., there was a big concentration gradient across the cell membrane. After injury Cr rose, but from 1·5 to 5 hours after injury only at less than 1 μg/(ml red cells)/min. The Cr/Cp ratio was close to 0·3, as in controls. The slowness of the changes in Cr and the maintenance of a high glucose concentration gradient across the red cell membrane indicated that the transport of glucose was normal in most of the 86% of the cells remaining in the circulation. Injury increased the rate of turnover of glucose, estimated from experiments with [U-14C] glucose, from 32 to 42 μg/(ml red cells)/min. This moderate increase was thought to be in the rate of glycolysis and to be a normal consequence of the increase in Cr after injury.

It was concluded that most of the red cells remaining in the circulation after a burn injury in the rat transported and metabolized glucose normally for up to 5 hours.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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