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. 1973 Feb;54(1):79–89.

Proliferation and Migration of Reticuloendothelial Cells Following Injection of a Tubercle Bacillary Lipid

K J Donald, A W Pound
PMCID: PMC2072500  PMID: 4632123

Abstract

A single intravenous injection of an adjuvant tubercle bacillary lipid into rabbits caused the number of circulating mononuclear cells in the blood to decrease from 1 to 8 hours and to increase from 24 to 48 hours after the lipid. The number of reticuloendothelial cells in spleen, liver and lung incorporating [3H]thymidine into the nuclei increased after 24 hours; the cells concerned in the lung were monocytes that formed developing granulomata. A single injection of the lipid into the footpad caused intense hyperplasia of the reticuloendothelial cells of the sinusoids of the draining lymph node. The reticuloendothelial cell hyperplasia reached a peak at 36 hours and persisted to 72 hours.

Multiple injections of the lipid caused a very great increase in number of reticuloendothelial cells in the liver and spleen. The clearance rate of colloidal carbon injected into such animals was increased 4 and 12 hours after the last injection of the lipid but there was no early accelerated phase of removal such as follows at this time after a single injection. After 48 hours the clearance rate of carbon was again normal. Study of the distribution of the carbon in the liver suggested that the accelerated rate of removal at the 4- and 12-hour intervals was due to an increased number of actively phagocytic cells and that at 48 hours some of these cells had become inactivated or otherwise lost.

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84-3Figs. 7-10
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84-1Figs. 3, 4 and 6
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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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