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. 1973 Oct;54(5):509–517.

The Migration of Rat Thoracic Duct Lymphocytes Through Spleen in vivo

J W B Bradfield, G V R Born
PMCID: PMC2072570  PMID: 4758381

Abstract

Rat thoracic duct lymphocytes have been labelled in vitro using [3H]5-uridine. After injection intravenously into syngeneic recipients their fate has been followed by scintillation counting and autoradiography. During the first 24 hours the distribution of radioactivity between different organs changed greatly with time. Lungs and liver contained about 50% of the injected radioactivity within 0·5 hours and lost most of this within 4 hours. In the spleen the radioactivity increased for about 4-6 hours and then decreased, whereas that in mesenteric and peripheral lymph nodes increased almost linearly for 24 hours. Free [3H]uridine and heat-killed cells failed to accumulate in lymphoid organs. When labelled lymphocytes were injected into splenectomized animals their lymph nodes accumulated more radioactivity earlier than the controls and remained more radioactive for at least 24 hours. It seems that one population of lymphocytes which accumulates in the rat spleen is released to accumulate subsequently in lymph nodes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bainbridge D. R., Gowland G. Studies on transplantation immunity. 1. The nature of the response to allogeneic 51 Cr-labeled lymphoid cells and its relationship to homograft immunity. Cell Immunol. 1971 Apr;2(2):115–127. doi: 10.1016/0008-8749(71)90031-1. [DOI] [PubMed] [Google Scholar]
  2. Cooper H. L., Kay J. E. Differential extraction of nuclear and cytoplasmic RNA from intact lymphocytes. Biochim Biophys Acta. 1969 Feb 18;174(2):503–512. doi: 10.1016/0005-2787(69)90279-2. [DOI] [PubMed] [Google Scholar]
  3. Ford W. L., Gowans J. L. The traffic of lymphocytes. Semin Hematol. 1969 Jan;6(1):67–83. [PubMed] [Google Scholar]
  4. Ford W. L. The immunological and migratory properties of the lymphocytes recirculating through the rat spleen. Br J Exp Pathol. 1969 Jun;50(3):257–269. [PMC free article] [PubMed] [Google Scholar]
  5. GESNER B. M., GINSBURG V. EFFECT OF GLYCOSIDASES ON THE FATE OF TRANSFUSED LYMPHOCYTES. Proc Natl Acad Sci U S A. 1964 Sep;52:750–755. doi: 10.1073/pnas.52.3.750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
  7. GOWANS J. L. The recirculation of lymphocytes from blood to lymph in the rat. J Physiol. 1959 Apr 23;146(1):54–69. doi: 10.1113/jphysiol.1959.sp006177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gesner B. M., Woodruff J. J., McCluskey R. T. An autoradiographic study of the effect of neuraminidase or trypsin on transfused lymphocytes. Am J Pathol. 1969 Nov;57(2):215–230. [PMC free article] [PubMed] [Google Scholar]
  9. Goldschneider I., McGregor D. D. Migration of lymphocytes and thymocytes in the rat. I. The route of migration from blood to spleen and lymph nodes. J Exp Med. 1968 Jan 1;127(1):155–168. doi: 10.1084/jem.127.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goldschneider I., McGregor D. D. Migration of lymphocytes and thymocytes in the rat. II. Circulation of lymphocytes and thymocytes from blood to lymph. Lab Invest. 1968 Apr;18(4):397–406. [PubMed] [Google Scholar]
  11. Griscelli C., Vassalli P., McCluskey R. T. The distribution of large dividing lymph node cells in syngeneic recipient rats after intravenous injection. J Exp Med. 1969 Dec 1;130(6):1427–1451. doi: 10.1084/jem.130.6.1427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hall J. G., Parry D. M., Smith M. E. The distribution and differentiation of lymph-borne immunoblasts after intravenous injection into syngeneic recipients. Cell Tissue Kinet. 1972 May;5(3):269–281. doi: 10.1111/j.1365-2184.1972.tb00365.x. [DOI] [PubMed] [Google Scholar]
  13. Howard J. C., Hunt S. V., Gowans J. L. Identification of marrow-derived and thymus-derived small lymphocytes in the lymphoid tissue and thoracic duct lymph of normal rats. J Exp Med. 1972 Feb 1;135(2):200–219. doi: 10.1084/jem.135.2.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Howard J. C. The life-span and recirculation of marrow-derived small lymphocytes from the rat thoracic duct. J Exp Med. 1972 Feb 1;135(2):185–199. doi: 10.1084/jem.135.2.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MACKANESS G. B. The action of drugs on intracellular tubercle bacilli. J Pathol Bacteriol. 1952 Jul;64(3):429–446. doi: 10.1002/path.1700640302. [DOI] [PubMed] [Google Scholar]
  16. Mitchell J. Lymphocyte circulation in the spleen. Marginal zone bridging channels and their possible role in cell traffic. Immunology. 1973 Jan;24(1):93–107. [PMC free article] [PubMed] [Google Scholar]
  17. Parrott D. M., De Sousa M. Thymus-dependent and thymus-independent populations: origin, migratory patterns and lifespan. Clin Exp Immunol. 1971 May;8(5):663–684. [PMC free article] [PubMed] [Google Scholar]
  18. Roser B., Ford W. L. Prolonged lymphocytopenia in the rat. The depletion of blood and thoracic duct lymphocyte populations following injection of -emitting colloids into the spleen or lymph nodes. Aust J Exp Biol Med Sci. 1972 Apr;50(2):165–184. [PubMed] [Google Scholar]
  19. WEISBERGER A. S., GUYTON R. A., HEINLE R. W., STORAASLI J. P. The role of the lungs in the removal of transfused lymphocytes. Blood. 1951 Oct;6(10):916–925. [PubMed] [Google Scholar]
  20. Woodruff J. J., Gesner B. M. The effect of neuraminidase on the fate of transfused lymphocytes. J Exp Med. 1969 Mar 1;129(3):551–567. doi: 10.1084/jem.129.3.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Zatz M. M., Lance E. M. The distribution of chromium 51-labelled lymphoid cells in the mouse. A survey of anatomical compartments. Cell Immunol. 1970 May;1(1):3–17. doi: 10.1016/0008-8749(70)90057-2. [DOI] [PubMed] [Google Scholar]

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