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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1974 Jun;55(3):251–259.

Some Observations on Liver Cell Proliferation in the Isolated Perfused Rat Liver

E A Lloyd, N Crozier, G Pamphlet, M Wells, S J Saunders
PMCID: PMC2072634  PMID: 4417181

Abstract

Experiments were designed to test the hypothesis that in an isolated perfused system a regenerating liver is able to stimulate a normal liver to increase DNA replication after cross-circulation for one hour. The high degree of purity of DNA extracted by the “phenol” technique is demonstrated and the suitability of this technique for rat livers emphasized. The effect of chemiluminescence on tritiated thymidine is presented.

The results of experiments presented here, together with other evidence, indicate the improbability of a stimulus being able to induce increased DNA synthesis in a normal liver after an exposure of only one hour.

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Selected References

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  1. BUCHER N. L. R., SCOTT J. F., AUB J. C. Regeneration of the liver in parabiotic rats. Cancer Res. 1951 Jun;11(6):457–465. [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bucher N. L., Schrock T. R., Moolten F. L. An experimental view of hepatic regeneration. Johns Hopkins Med J. 1969 Nov;125(5):250–257. [PubMed] [Google Scholar]
  4. FRICK G. A critical study of some commonly used methods of preparing desoxyribonucleate. Biochim Biophys Acta. 1954 Mar;13(3):374–380. doi: 10.1016/0006-3002(54)90344-3. [DOI] [PubMed] [Google Scholar]
  5. Fisher B., Szuch P., Levine M., Fisher E. R. A portal blood factor as the humoral agent in liver regeneration. Science. 1971 Feb 12;171(3971):575–577. doi: 10.1126/science.171.3971.575. [DOI] [PubMed] [Google Scholar]
  6. Hays D. M., Tedo I., Matsushima Y. Stimulation of in-vitro growth of rat liver cells with calf serum drawn following partial hepatectomy. J Surg Res. 1969 Mar;9(3):133–137. doi: 10.1016/0022-4804(69)90043-2. [DOI] [PubMed] [Google Scholar]
  7. Hems R., Ross B. D., Berry M. N., Krebs H. A. Gluconeogenesis in the perfused rat liver. Biochem J. 1966 Nov;101(2):284–292. doi: 10.1042/bj1010284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. JONES A. S., MARSH G. E. The deproteinisation of nucleoproteins. Biochim Biophys Acta. 1954 Aug;14(4):559–566. doi: 10.1016/0006-3002(54)90236-x. [DOI] [PubMed] [Google Scholar]
  9. KIRBY K. S. The preparation of deoxyribonucleic acids by the p-aminosalicylate-phenol method. Biochim Biophys Acta. 1959 Nov;36:117–124. doi: 10.1016/0006-3002(59)90075-7. [DOI] [PubMed] [Google Scholar]
  10. LEONG G. F., GRISHAM J. W., HOLE B. V., ALBRIGHT M. L. EFFECT OF PARTIAL HEPATECTOMY ON DNA SYNTHESIS AND MITOSIS IN HETEROTOPIC PARTIAL AUTOGRAFTS OF RAT LIVER. Cancer Res. 1964 Sep;24:1496–1501. [PubMed] [Google Scholar]
  11. Levi J. U., Zeppa R. Source of the humoral factor that initiates hepatic regeneration. Ann Surg. 1971 Sep;174(3):364–370. doi: 10.1097/00000658-197109000-00005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MASTER R. W. POSSIBLE SYNTHESIS OF POLYRIBONUCLEOTIDES OF KNOWN BASE-TRIPLET SEQUENCES. Nature. 1965 Apr 3;206:93–93. doi: 10.1038/206093b0. [DOI] [PubMed] [Google Scholar]
  13. Mayfield J. E., Bonner J. A partial sequence of nuclear events in regenerating rat liver (gene regulation-chromosomal RNA). Proc Natl Acad Sci U S A. 1972 Jan;69(1):7–10. doi: 10.1073/pnas.69.1.7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Moolten F. L., Bucher N. L. Regeneration of rat liver: transfer of humoral agent by cross circulation. Science. 1967 Oct 13;158(3798):272–274. doi: 10.1126/science.158.3798.272. [DOI] [PubMed] [Google Scholar]
  15. Morley C. G., Kingdon H. S. Use of 3 H-thymidine for measurement of DNA synthesis in rat liver--a warning. Anal Biochem. 1972 Jan;45(1):298–305. doi: 10.1016/0003-2697(72)90030-9. [DOI] [PubMed] [Google Scholar]
  16. PASCHKIS K. E. Growth-promoting factors in tissues: a review. Cancer Res. 1958 Oct;18(9):981–991. [PubMed] [Google Scholar]
  17. Paul D., Leffert H., Sato G., Holley R. W. Stimulation of DNA and protein synthesis in fetal-rat liver cells by serum from partially hepatectomized rats. Proc Natl Acad Sci U S A. 1972 Feb;69(2):374–377. doi: 10.1073/pnas.69.2.374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SIGEL B., ACEVEDO F. J., DUNN M. R. The effect of patial hepatectomy on autotransplanted liver tissue. Surg Gynecol Obstet. 1963 Jul;117:29–36. [PubMed] [Google Scholar]
  19. SIGEL B., DUNN M. R., BUTTERFIELD J. EFFECT OF PARTIAL HEPATECTOMY AND ECK FISTULA ON AUTOTRANSPLANTED LIVER TISSUE. EVIDENCE FOR A HUMORAL MECHANISM IN LIVER REGENERATION. Surg Forum. 1963;14:72–74. [PubMed] [Google Scholar]
  20. SMYTHE R. L., MOORE R. O. A study of possible humoral factors in liver regeneration in the rat. Surgery. 1958 Sep;44(3):561–569. [PubMed] [Google Scholar]
  21. Sakai A. Humoral factor triggering DNA synthesis after partial hepatectomy in the rat. Nature. 1970 Dec 19;228(5277):1186–1187. doi: 10.1038/2281186a0. [DOI] [PubMed] [Google Scholar]
  22. Sigel B., Baldia L. B., Brightman S. A., Dunn M. R., Price R. I. Effect of blood flow reversal in liver autotransplants upon the site of hepatocyte regeneration. J Clin Invest. 1968 Jun;47(6):1231–1237. doi: 10.1172/JCI105815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. VIROLAINEN M. MITOTIC RESPONSE IN LIVER AUTOGRAFT AFTER PARTIAL HEPATECTOMY IN THE RAT. Exp Cell Res. 1964 Feb;33:588–591. doi: 10.1016/0014-4827(64)90027-8. [DOI] [PubMed] [Google Scholar]
  24. WENNEKER A. S., SUSSMAN N. Regeneration of liver tissue following partial hepatectomy in parabiotic rats. Proc Soc Exp Biol Med. 1951 Apr;76(4):683–686. doi: 10.3181/00379727-76-18594. [DOI] [PubMed] [Google Scholar]
  25. WRBA H., RABES H., RIPOLL-GOMEZ M., RANZ H. [The metabolism-increasing effect of serum from partially hepatectomized animals in liver cultures]. Exp Cell Res. 1962 Feb;26:70–77. doi: 10.1016/0014-4827(62)90203-3. [DOI] [PubMed] [Google Scholar]

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