Skip to main content
PMC home page
British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1974 Feb;55(1):20–25.

The Maintenance of Liver Cells in vitro for Virological and Other Studies

D Rubenstein, M R Baker, E J Stott, A S Tavill
PMCID: PMC2072660  PMID: 4365222

Abstract

Cells from the livers of rats and monkeys were dispersed by enzyme treatment and maintained for weeks in roller tube cultures. Rat liver cells were shown to synthesize urea and monkey cells apparently synthesized RNA and protein—rat cells supported the growth of Sendai virus and monkey cells supported yellow fever virus. Organ cultures of human embryo liver supported the growth of adenovirus type 5 and herpes simplex virus. The Australia antigen serum hepatitis agent failed to grow in human or monkey liver cultures.

Full text

PDF
20

Images in this article

22-1Figs. 1-2
on p.22-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almeida J. D., Rubenstein D., Stott E. J. New antigen-antibody system in Australia-antigen-positive hepatitis. Lancet. 1971 Dec 4;2(7736):1225–1227. doi: 10.1016/s0140-6736(71)90543-5. [DOI] [PubMed] [Google Scholar]
  2. Campbell A. K., Hales C. N. Maintenance of viable cells in an organ culture of mature rat liver. Exp Cell Res. 1971 Sep;68(1):33–42. doi: 10.1016/0014-4827(71)90583-0. [DOI] [PubMed] [Google Scholar]
  3. East A. G., Louis L. N., Hoffenberg R. Albumin synthesis by isolated rat liver cells. Exp Cell Res. 1973 Jan;76(1):41–46. doi: 10.1016/0014-4827(73)90416-3. [DOI] [PubMed] [Google Scholar]
  4. Howard R. B., Pesch L. A. Respiratory activity of intact, isolated parenchymal cells from rat liver. J Biol Chem. 1968 Jun 10;243(11):3105–3109. [PubMed] [Google Scholar]
  5. Krebs H. A. Urea formation in mammalian liver. Biochem J. 1942 Dec;36(10-12):758–767. doi: 10.1042/bj0360758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Räihä N. C., Suihkonen J. Factors influencing the development of urea-synthesizing enzymes in rat liver. Biochem J. 1968 May;107(6):793–797. doi: 10.1042/bj1070793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Tavill A. S., Craigie A., Rosenoer W. M. The measurement of the synthetic rate of albumin in man. Clin Sci. 1968 Feb;34(1):1–28. [PubMed] [Google Scholar]
  8. Tavill A. S., Nadkarni D., Metcalfe J., Black E., Hoffenberg R., Carson E. R. (14C) carbonate kinetics and albumin synthesis by the isolated perfused rat liver. Clin Sci. 1973 Jul;45(1):3P–3P. doi: 10.1042/cs045003p. [DOI] [PubMed] [Google Scholar]
  9. Tyrrell D. A., Coid C. R. Sendai virus infection of rats as a convenient model of acute respiratory infection. Vet Rec. 1970 Feb 7;86(6):164–165. doi: 10.1136/vr.86.6.164. [DOI] [PubMed] [Google Scholar]
  10. Visek W. J. Effects of urea hydrolysis on cell life-span and metabolism. Fed Proc. 1972 May-Jun;31(3):1178–1193. [PubMed] [Google Scholar]
  11. Zuckerman A. J., Baines P. M., Almeida J. D. Australia antigen as a marker of propagation of the serum hepatitis virus in liver cultures. Nature. 1972 Mar 10;236(5341):78–81. doi: 10.1038/236078a0. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Experimental Pathology are provided here courtesy of Wiley

RESOURCES