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. 1974 Oct;55(5):478–486.

Human Placental Heteroantigens Demonstrable by Immunodiffusion

J M Gaugas, C Wright, P Curzen
PMCID: PMC2072679  PMID: 4281315

Abstract

Heteroantisera were raised against human placental homogenate (PH), or microsomal fraction (MF) which assumingly contained much trophoblast plasma membrane. Anti-PH serum included a readily demonstrable antibody against a fairly soluble mucopolysaccharide (PMA) embodied in the placenta. Anti-MF serum contained antibodies against 4 demonstrable antigens (designated IPA-a, IPA-b, IPA-c and IPA-H) in solubilized MF. Solubilized MF was fractionated in order to partially isolate and characterize the antigens which were apparently glycoproteins (molecular weight range of 800,000-30,000), Whereas solubilization achieved in a Triton X-100 solution followed by sonication was virtually complete (yield 95-100%), papain enzymatic digestion (yield 40-50%) released proportionately more of the lower molecular weight components (160,000-30,000) which incorporated IPA-c and IPA-H antigens. Antigen IPA-H bound to concanavalin A and so could be partially purified by affinity chromatography. Antigens IPA-a, IPA-b and IPA-H were shared by kidney MF, but IPA-c and PMA might be tissue specific.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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