Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1991 Feb;173(4):1382–1387. doi: 10.1128/jb.173.4.1382-1387.1991

Analysis of Mycoplasma hyorhinis DNA in the presence of host cells without growing the mycoplasma axenically.

W I Schaeffer 1, L D Olson 1, M F Barile 1, F W Sun 1
PMCID: PMC207274  PMID: 1671671

Abstract

Mycoplasma hyorhinis coisolates with the mitochondria of the cells in which it is carried as an infection. Since both mitochondria and mycoplasmas synthesize DNA by using the prokaryotic DNA polymerase gamma, the use of aphidicolin, which inhibits eukaryotic DNA polymerase alpha, allows for selective synthesis of mycoplasmal and mitochondrial DNA. The restriction patterns of mitochondria and mycoplasmas can easily be differentiated from each other in mixtures of both DNAs. Thus, it is possible to study the molecular biology of this noncultivable mycoplasma in situ rather than after growth in artificial media, with its potential genetic consequences during adjustment to axenic growth.

Full text

PDF
1382

Images in this article

1384Image
on p.1384
1384Image
on p.1384
1385Image
on p.1385
1385Image
on p.1385
1385Image
on p.1385

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bogenhagen D., Clayton D. A. The number of mitochondrial deoxyribonucleic acid genomes in mouse L and human HeLa cells. Quantitative isolation of mitochondrial deoxyribonucleic acid. J Biol Chem. 1974 Dec 25;249(24):7991–7995. [PubMed] [Google Scholar]
  2. Bustamante E., Soper J. W., Pedersen P. L. A high-yield preparative method for isolation of rat liver mitochondria. Anal Biochem. 1977 Jun;80(2):401–408. doi: 10.1016/0003-2697(77)90661-3. [DOI] [PubMed] [Google Scholar]
  3. Chandler D. K., Razin S., Stephens E. B., Harasawa R., Barile M. F. Genomic and phenotypic analyses of Mycoplasma pneumoniae strains. Infect Immun. 1982 Nov;38(2):604–609. doi: 10.1128/iai.38.2.604-609.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Darai G., Zöller L., Matz B., Delius H., Speck P. T., Flügel R. M. Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy. J Bacteriol. 1982 May;150(2):788–794. doi: 10.1128/jb.150.2.788-794.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hay R. J., Macy M. L., Chen T. R. Mycoplasma infection of cultured cells. Nature. 1989 Jun 8;339(6224):487–488. doi: 10.1038/339487a0. [DOI] [PubMed] [Google Scholar]
  6. Lo S. C., Shih J. W., Newton P. B., 3rd, Wong D. M., Hayes M. M., Benish J. R., Wear D. J., Wang R. Y. Virus-like infectious agent (VLIA) is a novel pathogenic mycoplasma: Mycoplasma incognitus. Am J Trop Med Hyg. 1989 Nov;41(5):586–600. doi: 10.4269/ajtmh.1989.41.586. [DOI] [PubMed] [Google Scholar]
  7. McGarrity G. J., Vanaman V., Sarama J. Cytogenetic effects of mycoplasmal infection of cell cultures: a review. In Vitro. 1984 Jan;20(1):1–18. doi: 10.1007/BF02633326. [DOI] [PubMed] [Google Scholar]
  8. Razin S., Barile M. F., Harasawa R., Amikam D., Glaser G. Characterization of the mycoplasma genome. Yale J Biol Med. 1983 Sep-Dec;56(5-6):357–366. [PMC free article] [PubMed] [Google Scholar]
  9. Razin S. Molecular biology and genetics of mycoplasmas (Mollicutes). Microbiol Rev. 1985 Dec;49(4):419–455. doi: 10.1128/mr.49.4.419-455.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Saillard C., Carle P., Bové J. M., Bébéar C., Lo S. C., Shih J. W., Wang R. Y., Rose D. L., Tully J. G. Genetic and serologic relatedness between Mycoplasma fermentans strains and a mycoplasma recently identified in tissues of AIDS and non-AIDS patients. Res Virol. 1990 May-Jun;141(3):385–395. doi: 10.1016/0923-2516(90)90010-g. [DOI] [PubMed] [Google Scholar]
  11. Schaeffer W. I. The long-term culture of a diploid rat hepatocyte cell strain. Ann N Y Acad Sci. 1980;349:165–182. doi: 10.1111/j.1749-6632.1980.tb29524.x. [DOI] [PubMed] [Google Scholar]
  12. Stephens E. B., Aulakh G. S., Rose D. L., Tully J. G., Barile M. F. Intraspecies genetic relatedness among strains of Acholeplasma laidlawii and of Acholeplasma axanthum by nucleic acid hybridization. J Gen Microbiol. 1983 Jun;129(6):1929–1934. doi: 10.1099/00221287-129-6-1929. [DOI] [PubMed] [Google Scholar]
  13. Tzagoloff A., Myers A. M. Genetics of mitochondrial biogenesis. Annu Rev Biochem. 1986;55:249–285. doi: 10.1146/annurev.bi.55.070186.001341. [DOI] [PubMed] [Google Scholar]
  14. Zimmermann W., Chen S. M., Bolden A., Weissbach A. Mitochondrial DNA replication does not involve DNA polymerase alpha. J Biol Chem. 1980 Dec 25;255(24):11847–11852. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES