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. 1991 Feb;173(4):1554–1560. doi: 10.1128/jb.173.4.1554-1560.1991

Characterization of the late-gene regulatory region of phage 21.

H C Guo 1, M Kainz 1, J W Roberts 1
PMCID: PMC207295  PMID: 1704887

Abstract

A segment of Escherichia coli bacteriophage 21 DNA encoding the late-gene regulator, Q21, and the late-gene leader RNA segment was sequenced; its structure is similar to those of the related phages lambda and 82. The leader RNA is about 45 nucleotides long and consists essentially entirely of sequences encoding the p-independent terminator that is the putative target of the antitermination activity of Q21. Like the corresponding regions of lambda and 82, the 21 late-gene promoter segment encodes an early transcription pause in vitro, at about nucleotide 18, during which Q21 presumably acts to modify RNA polymerase. The 21 Q gene, cloned in isolation, is active on the late-gene leader segment in trans, and its purified product is active as an antiterminator in vitro; Q21 represents a third late-gene antiterminator, in addition to those of lambda and 82. There is little evident similarity in the primary sequences of the three Q genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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