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. 1991 Mar;173(5):1810–1812. doi: 10.1128/jb.173.5.1810-1812.1991

UGA can be decoded as tryptophan at low efficiency in Bacillus subtilis.

P S Lovett 1, N P Ambulos Jr 1, W Mulbry 1, N Noguchi 1, E J Rogers 1
PMCID: PMC207334  PMID: 1900283

Abstract

Replacement of cat-86 codon 7 or 144 with the UGA codon permitted the gene to confer chloramphenicol resistance in wild-type Bacillus subtilis. UAA replacements of the same codons resulted in a chloramphenicol-sensitive phenotype in wild-type B. subtilis and a chloramphenicol-resistant phenotype in suppressor-positive strains. N-terminal sequencing showed that UGA at codon 7 was decoded as tryptophan in wild-type cells, at an efficiency of about 6%.

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Selected References

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