Abstract
In Kohara's library derived from Escherichia coli K-12 W3110 (Y. Kohara, K. Akiyama, and K. Isono, Cell 50:495-508, 1987), multiple copies of chromosomal sequence are found at 68 and at 64 to 65 min (M. Umeda and E. Ohtsubo, J. Mol. Biol. 213:229-237, 1990). We have determined that the rnpB gene (previously mapped at 70 min [B. J. Bachmann, Microbiol. Rev. 54:130-197, 1990]) is located within these segments of repeated sequences as five separate copies, together with tdcA, B, C, and R (mapped at 68 min [Bachmann, 1990]) and six unidentified open reading frames. Since close linkage of rnpB and tdc is found in various strains of E. coli K-12, the rnpB gene should be mapped at 68 min rather than 70 min.
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- Fujita Y., Fujita T., Miwa Y., Nihashi J., Aratani Y. Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis. J Biol Chem. 1986 Oct 15;261(29):13744–13753. [PubMed] [Google Scholar]
- Kohara Y., Akiyama K., Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987 Jul 31;50(3):495–508. doi: 10.1016/0092-8674(87)90503-4. [DOI] [PubMed] [Google Scholar]
- Komine Y., Adachi T., Inokuchi H., Ozeki H. Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12. J Mol Biol. 1990 Apr 20;212(4):579–598. doi: 10.1016/0022-2836(90)90224-A. [DOI] [PubMed] [Google Scholar]
- Motamedi H., Lee Y., Schmidt F. J. Tandem promoters preceding the gene for the M1 RNA component of Escherichia coli ribonuclease P. Proc Natl Acad Sci U S A. 1984 Jul;81(13):3959–3963. doi: 10.1073/pnas.81.13.3959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nasoff M. S., Baker H. V., 2nd, Wolf R. E., Jr DNA sequence of the Escherichia coli gene, gnd, for 6-phosphogluconate dehydrogenase. Gene. 1984 Mar;27(3):253–264. doi: 10.1016/0378-1119(84)90070-2. [DOI] [PubMed] [Google Scholar]
- Reed R. E., Altman S. Repeated sequences and open reading frames in the 3' flanking region of the gene for the RNA subunit of Escherichia coli ribonuclease P. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5359–5363. doi: 10.1073/pnas.80.17.5359. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sakano H., Yamada S., Ikemura T., Shimura Y., Ozeki H. Temperature sensitive mutants of Escherichia coli for tRNA synthesis. Nucleic Acids Res. 1974 Mar;1(3):355–371. doi: 10.1093/nar/1.3.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schweizer H. P., Datta P. Genetic analysis of the tdcABC operon of Escherichia coli K-12. J Bacteriol. 1988 Nov;170(11):5360–5363. doi: 10.1128/jb.170.11.5360-5363.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schweizer H. P., Datta P. Physical map location of the tdc operon of Escherichia coli. J Bacteriol. 1990 Jun;172(6):2825–2825. doi: 10.1128/jb.172.6.2825.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schweizer H. P., Datta P. The complete nucleotide sequence of the tdc region of Escherichia coli. Nucleic Acids Res. 1989 May 25;17(10):3994–3994. doi: 10.1093/nar/17.10.3994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Umeda M., Ohtsubo E. Mapping of insertion element IS5 in the Escherichia coli K-12 chromosome. Chromosomal rearrangements mediated by IS5. J Mol Biol. 1990 May 20;213(2):229–237. doi: 10.1016/S0022-2836(05)80186-X. [DOI] [PubMed] [Google Scholar]