Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1991 Mar;173(5):1821–1823. doi: 10.1128/jb.173.5.1821-1823.1991

The ferredoxin-thioredoxin reductase variable subunit gene from Anacystis nidulans.

M Szekeres 1, M Droux 1, B B Buchanan 1
PMCID: PMC207337  PMID: 1705544

Abstract

The ferredoxin-thioredoxin reductase variable subunit gene of Anacystis nidulans was cloned, and its nucleotide sequence was determined. A single-copy 219-bp open reading frame encoded a protein of 73 amino acid residues, with a calculated Mr of 8,400. The monocistronic transcripts were represented in a 400-base and a less abundant 300-base mRNA form.

Full text

PDF
1821

Images in this article

1822Image
on p.1822
1822Image
on p.1822

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aota S., Gojobori T., Ishibashi F., Maruyama T., Ikemura T. Codon usage tabulated from the GenBank Genetic Sequence Data. Nucleic Acids Res. 1988;16 (Suppl):r315–r402. doi: 10.1093/nar/16.suppl.r315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Borbely G., Simoncsits A. 3'-Terminal conserved loops of 16S rRNAs from the cyanobacterium Synechococcus AN PCC 6301 and maize chloroplast differ only in two bases. Biochem Biophys Res Commun. 1981 Aug 14;101(3):846–852. doi: 10.1016/0006-291x(81)91827-1. [DOI] [PubMed] [Google Scholar]
  3. Crawford N. A., Droux M., Kosower N. S., Buchanan B. B. Evidence for function of the ferredoxin/thioredoxin system in the reductive activation of target enzymes of isolated intact chloroplasts. Arch Biochem Biophys. 1989 May 15;271(1):223–239. doi: 10.1016/0003-9861(89)90273-7. [DOI] [PubMed] [Google Scholar]
  4. Droux M., Jacquot J. P., Miginac-Maslow M., Gadal P., Huet J. C., Crawford N. A., Yee B. C., Buchanan B. B. Ferredoxin-thioredoxin reductase, an iron-sulfur enzyme linking light to enzyme regulation in oxygenic photosynthesis: purification and properties of the enzyme from C3, C4, and cyanobacterial species. Arch Biochem Biophys. 1987 Feb 1;252(2):426–439. doi: 10.1016/0003-9861(87)90049-x. [DOI] [PubMed] [Google Scholar]
  5. Droux M., Miginiac-Maslow M., Jacquot J. P., Gadal P., Crawford N. A., Kosower N. S., Buchanan B. B. Ferredoxin-thioredoxin reductase: a catalytically active dithiol group links photoreduced ferredoxin to thioredoxin functional in photosynthetic enzyme regulation. Arch Biochem Biophys. 1987 Jul;256(1):372–380. doi: 10.1016/0003-9861(87)90458-9. [DOI] [PubMed] [Google Scholar]
  6. Golden S. S., Brusslan J., Haselkorn R. Genetic engineering of the cyanobacterial chromosome. Methods Enzymol. 1987;153:215–231. doi: 10.1016/0076-6879(87)53055-5. [DOI] [PubMed] [Google Scholar]
  7. Hunkapiller M. W., Lujan E., Ostrander F., Hood L. E. Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis. Methods Enzymol. 1983;91:227–236. doi: 10.1016/s0076-6879(83)91019-4. [DOI] [PubMed] [Google Scholar]
  8. Knaff D. B. The regulatory role of thioredoxin in chloroplasts. Trends Biochem Sci. 1989 Nov;14(11):433–434. doi: 10.1016/0968-0004(89)90095-9. [DOI] [PubMed] [Google Scholar]
  9. McLane J. A., Pawelek J. M. Receptors for B-melanocyte-stimulating hormone exhibit positive cooperativity in synchronized melanoma cells. Biochemistry. 1988 May 17;27(10):3743–3747. doi: 10.1021/bi00410a033. [DOI] [PubMed] [Google Scholar]
  10. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Szewczyk B., Kozloff L. M. A method for the efficient blotting of strongly basic proteins from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose. Anal Biochem. 1985 Nov 1;150(2):403–407. doi: 10.1016/0003-2697(85)90528-7. [DOI] [PubMed] [Google Scholar]
  12. Tomioka N., Sugiura M. The complete nucleotide sequence of a 16S ribosomal RNA gene from a blue-green alga, Anacystis nidulans. Mol Gen Genet. 1983;191(1):46–50. doi: 10.1007/BF00330888. [DOI] [PubMed] [Google Scholar]
  13. Vieira J., Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 1987;153:3–11. doi: 10.1016/0076-6879(87)53044-0. [DOI] [PubMed] [Google Scholar]
  14. Vogeli G., Kaytes P. S. Amplification, storage, and replication of libraries. Methods Enzymol. 1987;152:407–415. doi: 10.1016/0076-6879(87)52047-x. [DOI] [PubMed] [Google Scholar]
  15. Wallace R. B., Miyada C. G. Oligonucleotide probes for the screening of recombinant DNA libraries. Methods Enzymol. 1987;152:432–442. doi: 10.1016/0076-6879(87)52050-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES