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. 1992 Nov;174(21):7044–7047. doi: 10.1128/jb.174.21.7044-7047.1992

Regulation of the Staphylococcus aureus plasmid pI258 mercury resistance operon.

L Chu 1, D Mukhopadhyay 1, H Yu 1, K S Kim 1, T K Misra 1
PMCID: PMC207386  PMID: 1400255

Abstract

Experiments involving fusion between the Staphylococcus aureus plasmid pI258-encoded mer operon and the reporter gene beta-lactamase, mutational analysis, and trans-complementation studies have shown that the merR gene of pI258, which shows DNA sequence similarity with known merR genes from other bacteria, regulates the expression of the mer operon in vivo. The merR gene product is a trans-acting protein that activates mer operon transcription in the presence of the inducers Hg2+ and Cd2+. A glutathione-S-transferase-MerR fusion protein specifically bound and protected a 27-nucleotide operator sequence from DNase I digestion. This operator sequence is highly homologous with mer operator sequences of other known systems.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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