Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Nov;174(22):7227–7234. doi: 10.1128/jb.174.22.7227-7234.1992

Physical map of the Methanobacterium thermoautotrophicum Marburg chromosome.

R Stettler 1, T Leisinger 1
PMCID: PMC207416  PMID: 1429448

Abstract

A physical map of the Methanobacterium thermoautotrophicum Marburg chromosome was constructed by using pulsed-field gel electrophoresis of restriction fragments generated by NotI, PmeI, and NheI. The order of the fragments was deduced from Southern blot hybridization of NotI fragment probes to various restriction digests and from partial digests. The derived map is circular, and the genome size was estimated to be 1,623 kb. Several cloned genes were hybridized to restriction fragments to locate their positions on the map. Genes coding for proteins involved in the methanogenic pathway were located on the same segment of the circular chromosome. In addition, the genomes of a variety of thermophilic Methanobacterium strains were treated with restriction enzymes and analyzed by pulsed-field gel electrophoresis. The sums of the fragment sizes varied from 1,600 to 1,728 kb among the strains, and widely different macrorestriction patterns were observed.

Full text

PDF
7227

Images in this article

7229Image
on p.7229
7230Image
on p.7230
7232Image
on p.7232

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bokranz M., Bäumner G., Allmansberger R., Ankel-Fuchs D., Klein A. Cloning and characterization of the methyl coenzyme M reductase genes from Methanobacterium thermoautotrophicum. J Bacteriol. 1988 Feb;170(2):568–577. doi: 10.1128/jb.170.2.568-577.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bokranz M., Klein A., Meile L. Complete nucleotide sequence of plasmid pME2001 of Methanobacterium thermoautotrophicum (Marburg). Nucleic Acids Res. 1990 Jan 25;18(2):363–363. doi: 10.1093/nar/18.2.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Charlebois R. L., Schalkwyk L. C., Hofman J. D., Doolittle W. F. Detailed physical map and set of overlapping clones covering the genome of the archaebacterium Haloferax volcanii DS2. J Mol Biol. 1991 Dec 5;222(3):509–524. doi: 10.1016/0022-2836(91)90493-p. [DOI] [PubMed] [Google Scholar]
  4. Chu G., Vollrath D., Davis R. W. Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science. 1986 Dec 19;234(4783):1582–1585. doi: 10.1126/science.3538420. [DOI] [PubMed] [Google Scholar]
  5. Cohen A., Lam W. L., Charlebois R. L., Doolittle W. F., Schalkwyk L. C. Localizing genes on the map of the genome of Haloferax volcanii, one of the Archaea. Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1602–1606. doi: 10.1073/pnas.89.5.1602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DiMarco A. A., Bobik T. A., Wolfe R. S. Unusual coenzymes of methanogenesis. Annu Rev Biochem. 1990;59:355–394. doi: 10.1146/annurev.bi.59.070190.002035. [DOI] [PubMed] [Google Scholar]
  7. DiMarco A. A., Sment K. A., Konisky J., Wolfe R. S. The formylmethanofuran:tetrahydromethanopterin formyltransferase from Methanobacterium thermoautotrophicum delta H. Nucleotide sequence and functional expression of the cloned gene. J Biol Chem. 1990 Jan 5;265(1):472–476. [PubMed] [Google Scholar]
  8. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  9. Grothues D., Tümmler B. New approaches in genome analysis by pulsed-field gel electrophoresis: application to the analysis of Pseudomonas species. Mol Microbiol. 1991 Nov;5(11):2763–2776. doi: 10.1111/j.1365-2958.1991.tb01985.x. [DOI] [PubMed] [Google Scholar]
  10. Halboth S., Klein A. Methanococcus voltae harbors four gene clusters potentially encoding two [NiFe] and two [NiFeSe] hydrogenases, each of the cofactor F420-reducing or F420-non-reducing types. Mol Gen Genet. 1992 May;233(1-2):217–224. doi: 10.1007/BF00587582. [DOI] [PubMed] [Google Scholar]
  11. Howard P. J., Harsono K. D., Luchansky J. B. Differentiation of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, and Listeria seeligeri by pulsed-field gel electrophoresis. Appl Environ Microbiol. 1992 Feb;58(2):709–712. doi: 10.1128/aem.58.2.709-712.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jenal U., Rechsteiner T., Tan P. Y., Bühlmann E., Meile L., Leisinger T. Isoleucyl-tRNA synthetase of Methanobacterium thermoautotrophicum Marburg. Cloning of the gene, nucleotide sequence, and localization of a base change conferring resistance to pseudomonic acid. J Biol Chem. 1991 Jun 5;266(16):10570–10577. [PubMed] [Google Scholar]
  13. Jones W. J., Nagle D. P., Jr, Whitman W. B. Methanogens and the diversity of archaebacteria. Microbiol Rev. 1987 Mar;51(1):135–177. doi: 10.1128/mr.51.1.135-177.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kauc L., Mitchell M., Goodgal S. H. Size and physical map of the chromosome of Haemophilus influenzae. J Bacteriol. 1989 May;171(5):2474–2479. doi: 10.1128/jb.171.5.2474-2479.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kiener A., König H., Winter J., Leisinger T. Purification and use of Methanobacterium wolfei pseudomurein endopeptidase for lysis of Methanobacterium thermoautotrophicum. J Bacteriol. 1987 Mar;169(3):1010–1016. doi: 10.1128/jb.169.3.1010-1016.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Klein A., Schnorr M. Genome complexity of methanogenic bacteria. J Bacteriol. 1984 May;158(2):628–631. doi: 10.1128/jb.158.2.628-631.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. López-García P., Abad J. P., Smith C., Amils R. Genomic organization of the halophilic archaeon Haloferax mediterranei: physical map of the chromosome. Nucleic Acids Res. 1992 May 25;20(10):2459–2464. doi: 10.1093/nar/20.10.2459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Meile L., Kiener A., Leisinger T. A plasmid in the archaebacterium Methanobacterium thermoautotrophicum. Mol Gen Genet. 1983;191(3):480–484. doi: 10.1007/BF00425766. [DOI] [PubMed] [Google Scholar]
  19. Meile L., Stettler R., Banholzer R., Kotik M., Leisinger T. Tryptophan gene cluster of Methanobacterium thermoautotrophicum Marburg: molecular cloning and nucleotide sequence of a putative trpEGCFBAD operon. J Bacteriol. 1991 Aug;173(16):5017–5023. doi: 10.1128/jb.173.16.5017-5023.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mitchell R. M., Loeblich L. A., Klotz L. C., Loeblich A. R., 3rd DNA organization of Methanobacterium thermoautotrophicum. Science. 1979 Jun 8;204(4397):1082–1084. doi: 10.1126/science.377486. [DOI] [PubMed] [Google Scholar]
  21. Noll K. M. Chromosome map of the thermophilic archaebacterium Thermococcus celer. J Bacteriol. 1989 Dec;171(12):6720–6725. doi: 10.1128/jb.171.12.6720-6725.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Nuijten P. J., Bartels C., Bleumink-Pluym N. M., Gaastra W., van der Zeijst B. A. Size and physical map of the Campylobacter jejuni chromosome. Nucleic Acids Res. 1990 Nov 11;18(21):6211–6214. doi: 10.1093/nar/18.21.6211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Reeve J. N., Beckler G. S. Conservation of primary structure in prokaryotic hydrogenases. FEMS Microbiol Rev. 1990 Dec;7(3-4):419–424. doi: 10.1111/j.1574-6968.1990.tb04947.x. [DOI] [PubMed] [Google Scholar]
  24. Rospert S., Linder D., Ellermann J., Thauer R. K. Two genetically distinct methyl-coenzyme M reductases in Methanobacterium thermoautotrophicum strain Marburg and delta H. Eur J Biochem. 1990 Dec 27;194(3):871–877. doi: 10.1111/j.1432-1033.1990.tb19481.x. [DOI] [PubMed] [Google Scholar]
  25. Sitzmann J., Klein A. Physical and genetic map of the Methanococcus voltae chromosome. Mol Microbiol. 1991 Feb;5(2):505–513. doi: 10.1111/j.1365-2958.1991.tb02134.x. [DOI] [PubMed] [Google Scholar]
  26. Smith C. L., Econome J. G., Schutt A., Klco S., Cantor C. R. A physical map of the Escherichia coli K12 genome. Science. 1987 Jun 12;236(4807):1448–1453. doi: 10.1126/science.3296194. [DOI] [PubMed] [Google Scholar]
  27. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES